IHAME

Agace karimo antigen ya MP-Ag ikingira ku gace k’igeragezwa n’antibody ya IgG irwanya imbeba y’ihene iri ku gace k’igenzura, gafatanye na chromatography ya membrane mbere. Agapapuro k’igenzura gatwikiriwe na colloidal gold yanditseho imbeba-anti human IgM McAb mbere. Mu gupima icyitegererezo cyagaragaje ko ari cyiza, MP-IgM iri mu gipimo ihurirana na colloidal gold yanditseho imbeba-anti human IgM McAb, kandi igakora immunocomplication. Mu gihe cy’imikorere ya immunochromatography, complex n’icyitegererezo biri imbere mu membrane ya nitrocellulose binyura mu cyerekezo cy’impapuro zifata, iyo complex yanyuze mu gace k’igerageza, yahujwe na antigen ya MP-Ag ikingira, ikora complex ya "MP-Ag ikingira antigen-MP-IgM-colloidal gold yanditseho imbeba-anti human IgM McAb", agace k’igerageza k’amabara kagaragaye ku gace k’igerageza. Icyitegererezo cya negatif ntigitanga agace k’igerageza bitewe n’uko immune complex idahagije. Uko MP-IgM yaba iri kose mu gipimo cyangwa idafite, hari umurongo utukura ugaragara ku gace k’igenzura ry’ubuziranenge, ufatwa nk’amahame y’ubwiza bw’imbere mu kigo.

Uburyo bwo gupima:

Uburyo bwo gupima WIZ-A101 reba amabwiriza ya mudasobwa ipima ubudahangarwa bw'umubiri. Uburyo bwo gupima amaso ni ubu bukurikira:

1. Shyira ku ruhande ibikoresho byose bitanga ubushyuhe n'ibipimo ku bushyuhe bw'icyumba.
2. Kura ikarita yo gupima mu gikapu cya foil, uyishyire ku meza iringaniye hanyuma uyishyireho akamenyetso.
3. Ongeramo 10μL serum cyangwa plasma cyangwa 20μL amaraso yose mu gice cy'ikarita kirimo dispette, hanyuma ongeramo 100μL (hafi igitonyanga 2-3) cy'icyitegererezo, igihe cyo gutangira.
4. Tegereza nibura iminota 10-15 hanyuma usome ibisubizo, ibisubizo biba nta gaciro bifite nyuma y'iminota 15.