IHAME

Agace karimo agapira ka McAb gakingira calcium ku gace k’igeragezwa n’agapira ka IgG gakingira ihene ku gace k’igenzura, gafatanye na membrane chromatography mbere. Agapapuro k’igenzura gatwikiriwe na colloidal gold gafite ikimenyetso cya anti cal McAb na colloidal gold gafite ikimenyetso cya IgG gafite ikimenyetso cya IgG gafite ikimenyetso cya IgAb. Mu gihe cyo gupima icyitegererezo cyanduye, cal iri mu gipimo ivanze na colloidal gold gafite ikimenyetso cya anti cal McAb, kandi igakora ubudahangarwa bw’umubiri, kuko yemerewe kwimuka ku gace k’igerageza, cal conjugate complex ifatwa na anti cal coating McAb ku gapira maze igakora "anti cal coating McAb-cal-colloidal gold gafite ikimenyetso cya anti cal McAb", agapira k’igerageza k’amabara kagaragaye ku gace k’igerageza. Ubukana bw’ibara bufitanye isano ryiza n’ibikubiye muri cal. Icyitegererezo cya negative ntigitanga agapira k’igerageza bitewe n’uko nta colloidal gold conjugate cal complex. Uko cal yaba iri kose mu gipimo cyangwa idafite, hari umurongo utukura ugaragara ku gace k’igenzura n’akarere k’igenzura ry’ubuziranenge, gafatwa nk’amahame y’ubwiza bw’imbere mu kigo.

Uburyo bwo gupima:

1. Kuramo agakoni k'ingero, kashyizwe mu gipimo cy'amase, hanyuma usubize agakoni k'ingero inyuma, ugateke neza hanyuma uzunguze neza, ongeramo igikorwa inshuro 3. Cyangwa ukoresheje agakoni k'ingero k'amase katoranijwemo 50mg, hanyuma ushyiremo umuyoboro w'ingero z'amase urimo icyitegererezo cyo gushonga, hanyuma ugateke neza.

2. Koresha isuzuma ry'imiyoboro ikoreshwa mu gupima, fata isuzuma ry'umwanda muto w'umurwayi w'impiswi, hanyuma ongeramo ibitonyanga 3 (hafi 100uL) mu muyoboro w'umwanda hanyuma uzunguze neza, ubishyire ku ruhande.

3. Kura ikarita yo gupima mu gikapu cya foil, uyishyire ku meza iringaniye hanyuma uyishyireho akamenyetso.

4. Kuraho umupfundikizo mu muyoboro w'icyitegererezo hanyuma ujugunye ibitonyanga bibiri bya mbere by'icyitegererezo cyavanzwe, ongeramo ibitonyanga 4 (hafi 100uL) by'icyitegererezo cyavanzwe kidafunze neza hanyuma buhoro buhoro ushyire mu iriba ry'icyitegererezo cy'ikarita hamwe n'igihe cyatanzwe, tangira igihe.

5. Ibisubizo bigomba gusomwa mu minota 10-15, kandi nyuma y'iminota 15 nta gaciro bifite