UMGAQO

I-strip ine-antigen ye-MP-Ag coating kwindawo yovavanyo kunye ne-antibody ye-IgG ye-mouse yebhokhwe kwindawo yokulawula, ebotshelelwe kwi-membrane chromatography kwangaphambili. I-Lable pad igqunywe ngegolide ye-colloidal ebhalwe i-mouse-anti human IgM McAb kwangaphambili. Xa kuvavanywa isampuli elungileyo, i-MP-IgM kwisampuli idibana negolide ye-colloidal ebhalwe i-mouse-anti human IgM McAb, kwaye yenze i-immune complex. Phantsi kwesenzo se-immunochromatography, i-complex kunye nesampuli engaphakathi kwe-nitrocellulose membrane zihamba kwicala lephepha elifunxayo, xa i-complex idlula kwindawo yovavanyo, idibene ne-antigen ye-MP-Ag coating, yenze i-complex ethi "MP-Ag coating antigen-MP-IgM-colloidal gold ebhalwe i-mouse-anti human IgM McAb", ibhendi yovavanyo enemibala yavela kwindawo yovavanyo. Isampuli engalunganga ayivelisi ibhendi yovavanyo ngenxa ye-immune complex engonelanga. Nokuba i-MP-IgM ikhona kwisampuli okanye ayikho, kukho umgca obomvu obonakalayo kwindawo yokulawula umgangatho, othathwa njengemigangatho yangaphakathi yeshishini yomgangatho.

Inkqubo yoVavanyo:

Inkqubo yovavanyo lwe-WIZ-A101 jonga imiyalelo ye-analyzer ye-immune ephathekayo. Inkqubo yovavanyo olubonakalayo yile ilandelayo:

1. Beka ecaleni zonke ii-reagents kunye neesampuli kubushushu begumbi.
2. Khupha ikhadi lovavanyo kwingxowa yefoyile, ulibeke etafileni elinganayo uze uliphawule.
3. Yongeza isampuli ye-serum okanye i-plasma eyi-10μL okanye isampuli yegazi elipheleleyo eyi-20μL ukuze usampulise umthombo wekhadi kunye ne-dispette enikiweyo, uze wongeze i-100μL (malunga ne-2-3 drop) yesampuli yokunyibilikisa, ixesha lokuqala.
4. Linda ubuncinane imizuzu eli-10-15 uze ufunde isiphumo, isiphumo asisebenzi emva kwemizuzu eli-15.