ISIMISO

Lo mcu une-antigen yokumboza i-MP-Ag endaweni yokuhlola kanye ne-antibody ye-IgG yegundane elilwa negundane endaweni yokulawula, eboshelwe ku-chromatography ye-membrane kusengaphambili. Iphedi yelebula imbozwe ngegolide le-colloidal elibhalwe nge-mouse-anti human IgM McAb kusengaphambili. Lapho kuhlolwa isampula enhle, i-MP-IgM esampula ihlangana ne-colloidal gold ebhalwe nge-mouse-anti human IgM McAb, bese yakha i-immune complex. Ngaphansi kwesenzo se-immunochromatography, i-complex kanye nesampula ngaphakathi kwe-membrane ye-nitrocellulose kugeleza ngasendleleni yephepha elimuncayo, lapho i-complex idlula endaweni yokuhlola, ihlanganiswe ne-antigen yokumboza i-MP-Ag, yakha i-complex ethi “MP-Ag coating antigen-MP-IgM-colloidal gold ebhalwe nge-mouse-anti human IgM McAb”, kwavela ibhendi yokuhlola enemibala endaweni yokuhlola. Isampula engemihle ayikhiqizi ibhendi yokuhlola ngenxa ye-immune complex engasebenzi kahle. Kungakhathaliseki ukuthi i-MP-IgM ikhona kusampula noma cha, kunomugqa obomvu obonakala esifundeni sokulawula ikhwalithi, okubhekwa njengezindinganiso zebhizinisi zangaphakathi zekhwalithi.

Inqubo Yokuhlola:

Inqubo yokuhlola ye-WIZ-A101 bheka imiyalelo ye-analyzer ye-immune ephathekayo. Inqubo yokuhlola okubonakalayo imi kanje:

1. Beka eceleni wonke ama-reagent namasampula endaweni yokushisa yegumbi.
2. Khipha ikhadi lokuhlola esikhwameni se-foil, ulibeke etafuleni elilinganayo bese ulimaka.
3. Engeza isampula ye-serum noma i-plasma engu-10μL noma isampula yegazi eliphelele elingu-20μL ukuze usampula umthombo wekhadi nge-dispette enikeziwe, bese ufaka isampula ye-diluent engu-100μL (cishe amaconsi angu-2-3), qala isikhathi.
4. Linda okungenani imizuzu eyi-10-15 bese ufunda umphumela, umphumela awusebenzi ngemva kwemizuzu eyi-15.