TE MATUA

Kei te rīpene he antigen paninga MP-Ag i te rohe whakamātautau me te paturopi anti-kiore IgG koati i te rohe whakahaere, e herea ana ki te chromatography membrane i mua. Ka pania te papa tapanga ki te koura colloidal kua tohua ko te kiore-anti-tangata IgM McAb i mua. I te whakamātautau i te tauira pai, ka honoa te MP-IgM i roto i te tauira ki te koura colloidal kua tohua ko te kiore-anti-tangata IgM McAb, ka hanga he matatini ārai mate. I raro i te mahi a te immunochromatography, ka rere te matatini me te tauira i roto i te membrane nitrocellulose i te ahunga o te pepa mimiti, i te wa i paahitia e te matatini te rohe whakamātautau, ka honoa ki te antigen paninga MP-Ag, ka hanga he matatini "MP-Ag paninga antigen-MP-IgM-colloidal koura kua tohua ko te kiore-anti-tangata IgM McAb", ka puta he roopu whakamātautau tae ki te rohe whakamātautau. Kāore he tauira kino e whakaputa i te roopu whakamātautau nā te ngoikore o te matatini ārai mate. Ahakoa kei roto te MP-IgM i te tauira, kāore rānei, ka puta he raina whero ki te rohe whakahaere kounga, e kiia ana ko ngā paerewa kounga ā-roto o te umanga.

Tukanga Whakamātautau:

Ko te tukanga whakamātautau WIZ-A101 e whai ana i ngā tohutohu o te taputapu tātari ārai mate kawe. Ko te tukanga whakamātautau tirohanga e whai ake nei:

1. Waiho ngā matū me ngā tauira katoa ki te pāmahana rūma.
2. Tangohia te kāri whakamātautau mai i te putea pepa konutea, whakatakotoria ki runga i te tēpu papatahi, ka tohua.
3. Tāpirihia he 10μL tauira toto, he tauira toto toto rānei, he 20μL tauira toto katoa rānei ki te puna tauira o te kāri me te dispette kua whakaratohia, kātahi ka tāpirihia he 100μL (tata ki te 2-3 pata) wai whakawera tauira, tīmatahia te wā.
4. Taria mō te iti rawa 10-15 meneti, kātahi ka pānui i te hua, kāore e whai mana te hua i muri i te 15 meneti.