TE MATUA

Kei te rīpene he paninga anti-cal McAb i te rohe whakamātautau me te paturopi anti-rāpeti koati IgG i te rohe whakahaere, e herea ana ki te chromatography membrane i mua. Ka pania te papa tapanga ki te koura colloidal kua tohua he anti-cal McAb me te paturopi rapeti kua tohua he koura colloidal IgG i mua. I te whakamātautau i te tauira pai, ka honoa te cal i roto i te tauira ki te koura colloidal kua tohua he anti-cal McAb, ka hanga he matatini ārai mate, i te mea ka tukuna kia heke i te rīpene whakamātautau, ka hopukina te matatini honohono cal e te paninga anti-cal McAb i runga i te membrane ka hanga he matatini "paninga anti-cal McAb-cal-colloidal koura kua tohua he anti-cal McAb", ka puta he roopu whakamātautau tae i te rohe whakamātautau. He hononga pai te kaha o te tae ki te ihirangi cal. Kāore he roopu whakamātautau e puta mai i te tauira kino nā te korenga o te matatini honohono koura colloidal. Ahakoa kei roto te cal i te tauira, kāore rānei, ka puta he raina whero i te rohe tohutoro me te rohe whakahaere kounga, e kiia ana ko ngā paerewa kounga ā-roto o te umanga.

Tukanga Whakamātautau:

1. Tangohia te rākau tauira kua whakaurua ki roto i te tauira paru, kātahi ka whakahokia te rākau tauira, ka whakapiripiri kia mau, ka rūrū kia pai, ka whakahokia te mahi kia 3 ngā wā. Mā te whakamahi rānei i te rākau tauira, ka kōwhiria te tauira paru kia 50mg pea, ka whakauru ki roto i te ngongo tauira paru kei roto te waimeha tauira, ka whakapiripiri kia mau.

2. Whakamahia he pipette tango tauira, tangohia te tauira paru angiangi mai i te tūroro mate korere, kātahi ka tāpirihia kia 3 ngā pata (tata ki te 100uL) ki te ngongo tauira paru, ka rūrū pai, ka waiho ki tahaki.

3. Tangohia te kāri whakamātautau mai i te putea pepa konutea, whakatakotoria ki runga i te tēpu papatahi, ka tohua.

4. Tangohia te taupoki i te ngongo tauira ka maka atu ngā pata tuatahi e rua o te tauira kua whakaranua, tāpirihia kia 4 ngā pata (tata ki te 100µL) o te tauira kua whakaranua kia poutū, ka ata ringihia ki roto i te poka tauira o te kāri me te putanga kua whakaratohia, tīmatahia te wā.

5. Me pānui te hua i roto i ngā meneti 10-15, ā, kāore e whai mana i muri i ngā meneti 15