Agakoresho ko gusuzuma indwara kageneweImisemburo itera imbaraga za tiroyideikizamini cya immunochromatographic)
Gukoreshwa mu gusuzuma indwara mu buryo bwa "in vitro" gusa
Mbere yo gukoresha iyi paki, soma witonze kandi ukurikize amabwiriza neza. Ntabwo bishoboka kwemeza ko ibisubizo by'isuzuma ari ukuri niba hari ibitagenda neza ku mabwiriza ari muri iyi paki.

IKORESHWA RYITEGANYANYE
Ikizamini cyo gusuzuma imisemburo itera indwara ya Thyroid (ikizamini cya fluorescence immunochromatographic assay) ni ikizamini cya fluorescence immunochromatographic assay cyo gupima ingano ya hormone itera indwara ya Thyroid (TSH) mu maraso y'umuntu cyangwa mu maraso, gikoreshwa cyane cyane mu gusuzuma imikorere ya pituitary-thyroid. Ikizamini cyose cyabonetse kigomba kwemezwa n'ubundi buryo. Iki kizamini kigenewe gukoreshwa n'abaganga gusa.

INSHAMAKE
Imirimo y'ingenzi ya TSH: 1, guteza imbere irekurwa ry'imisemburo ya tiroyide, 2, guteza imbere ikorwa rya T4 na T3, harimo kongera imbaraga mu ikoreshwa rya iyode, kongera imbaraga mu ikorwa rya peroxidase, guteza imbere ikorwa rya globulin ya tiroyide na iyode ya tyrosine

IHAME RY'UBURYO BWAYO
Agace k'igikoresho cyo gupima gatwikiriwe na antibody ya TSH ku gace k'igeragezwa naho antibody ya IgG ya antibody y'ihene ku gace k'igenzura. Udupapuro two gupima dutwikiriwe na antibody ya TSH yanditseho fluorescence na IgG ya rabbit mbere y'igihe. Mu gusuzuma icyitegererezo cyagaragaje ko ari cyiza, antigen ya TSH iri mu gipimo ihuzwa na antibody ya TSH yanditseho fluorescence, maze igakora uruvange rw'ubudahangarwa bw'umubiri. Mu gihe cy'imikorere ya immunochromatography, urujya n'uruza rw'impapuro zifata, iyo complex inyuze mu gace k'igeragezwa, ihujwe na antibody ya TSH, ikora complex nshya. Urwego rwa TSH rufitanye isano ryiza n'ikimenyetso cya fluorescence, kandi ingano ya TSH mu gipimo ishobora kumenyekana hakoreshejwe isuzuma ry'ubudahangarwa bw'umubiri rya fluorescence.

IBIKORESHO N'IBIKORESHO BYATANZWE

Ibice by'ipaki bya 25T
Ikarita yo gupima ku giti cyayo ipfunyitse mu gapfunyika gafite agakoresho ko gusukura 25T
.Ingero z'ibinyabutabire
.Ipaki yo gushyiramo

IBIKORESHO BIRAKENEWE ARIKO NTIBYATANGWA
Agasanduku ko gukusanya ingero, igihe

ICYITEGEREREZO CYO GUKORESHA NO KUBIKA
1. Ingero zapimwe zishobora kuba serum, heparin anticoagulant plasma cyangwa EDTA anticoagulant plasma.
2. Dukurikije uburyo busanzwe bwo gukusanya icyitegererezo. Icyitegererezo cya serum cyangwa plasma gishobora kubikwa muri firigo kuri 2-8°C mu gihe cy'iminsi 7 no kubikwa munsi ya -15°C mu gihe cy'amezi 6.
3. Ingero zose zirinda gukonjesha no gushonga.

UBURYO BWO GUKORA IKIGANIRO
Uburyo bwo gupima igikoresho reba igitabo cy'amabwiriza agenga ikoreshwa ry'imyunyungugu. Uburyo bwo gupima reagent ni ubu bukurikira

1. Shyira ku ruhande ibikoresho byose bikoreshwa mu gupima no mu gupima ingero ku bushyuhe bw'icyumba.
2. Fungura Portable Immune Analyzer (WIZ-A101), andika ijambo ry'ibanga ryinjira muri konti ukurikije uburyo bw'imikorere y'igikoresho, hanyuma winjize aho ureba.
3.Skena kode y'isuzuma kugira ngo wemeze ikintu cy'ikizamini.
4. Kura ikarita yo gupima mu gafuka k'ifishi.
5. Shyiramo ikarita y'ikizamini mu mwanya w'ikarita, skana kode ya QR, hanyuma umenye ikintu cy'ikizamini.
6. Ongeramo 20μL ya serum cyangwa plasma mu gipimo cy'icyitegererezo, hanyuma uvange neza.
7. Ongeraho umuti w'icyitegererezo wa 80μL ku iriba ry'ikarita.
8. Kanda kuri buto "ikizamini gisanzwe", nyuma y'iminota 15, igikoresho kizahita kibona ikarita y'ikizamini, gishobora gusoma ibisubizo bivuye kuri ecran y'igikoresho, no kwandika/gucapa ibisubizo by'ikizamini.
9. Reba amabwiriza ya Portable Immune Analyzer (WIZ-A101).

IBISUBIZO BY'IBIGERAGEZO N'UBUSOBANURO
.Amakuru yavuzwe haruguru ni ibyavuye mu isuzuma rya TSH reagent, kandi birasabwa ko buri laboratwari igomba gushyiraho ubwoko butandukanye bw'agaciro k'isuzuma rya TSH gakwiriye abaturage bo muri aka karere. Ibisubizo byavuzwe haruguru ni ibyo kwifashisha gusa.
Ibisubizo by'ubu buryo bireba gusa ingero z'ingero zashyizweho muri ubu buryo, kandi nta kugereranya kutaziguye n'ubundi buryo.
Izindi mpamvu zishobora gutera amakosa mu bisubizo byo kuvumbura, harimo impamvu za tekiniki, amakosa mu mikorere n'izindi mpamvu z'icyitegererezo.

UBUBIKO N'UBUTABE
1. Iyi mashini ishobora kumara amezi 18 ikoreshwa kuva ikozwe. Bika ibikoresho bitakoreshejwe kuri dogere selisiyusi 2-30. NTUBIKORESHE. Ntukabikoreshe nyuma y'itariki ntarengwa.
2. Ntugafungure agafuka gafunze kugeza igihe uzaba witeguye gukora ikizamini, kandi ikizamini gikoreshwa rimwe gusa ni byiza ko gikoreshwa mu gihe gikenewe (ubushyuhe 2-35℃, ubushuhe 40-90%) mu minota 60 vuba bishoboka.
3. Icyitegererezo cy'umuti uvangwa n'amazi gikoreshwa ako kanya nyuma yo gufungurwa.

IMIBURO N'IBYITONDERWA
.Isanduku igomba kuba ifunze kandi irinzwe ubushuhe.
Ingero zose z’ibyavuye mu bushakashatsi zigomba kwemezwa n’ubundi buryo.
Ibipimo byose bigomba gufatwa nk'ibintu bishobora kwanduza.
NTUKORESHE reagent yarengeje igihe.
NTUGAHINDURE ibyuma bihinduranya ibikoresho hagati y'ibikoresho bifite ubwoko butandukanye No.
NTUGAKORESHE gukoresha amakarita yo gupima n'ibindi bikoresho byo gukoresha mu gihe cyo kuyakoresha.
.Gukoresha nabi, icyitegererezo gikabije cyangwa gito bishobora gutuma ibisubizo bihinduka.

LKWIGANA
Nkuko bigenda ku isuzuma ryose rikoresha uturemangingo tw’imbeba, birashoboka ko uturemangingo tw’imbeba (HAMA) twangiritse mu gipimo. Ingero z’abarwayi bahawe imiti y’uturemangingo tw’imbeba kugira ngo bapimwe cyangwa bavurwe zishobora kuba zirimo HAMA. Ingero nk’izo zishobora gutera ibisubizo bitari byo by’ukuri cyangwa bitari ukuri.
Ibi bisubizo ni ibyo kwitabwaho mu buvuzi gusa, ntabwo bigomba kuba ishingiro ryonyine ryo gusuzuma no kuvura abarwayi, uburyo bwo kuvura abarwayi bugomba kwitabwaho mu buryo bwuzuye hamwe n'ibimenyetso byabo, amateka y'ubuzima bwabo, ubundi buryo bwo gusuzuma muri laboratwari, uko ubuvuzi bwaba buhagaze, ibyorezo by'indwara n'andi makuru.
Iyi reagent ikoreshwa gusa mu gupima amaraso mu maraso no mu maraso. Ishobora kutabona igisubizo nyacyo iyo ikoreshejwe mu bindi bipimo nk'amacandwe n'inkari n'ibindi.

IBIRANGA IMIKORERE

INYANDIKO Z'IBIREGO
1.Hansen JH, et al.HAMA Interference hamwe n'ibizamini by'imyuka bishingiye ku murine monoclonal antibody [J].J bya Clin Immunoassay, 1993,16:294-299.
2.Levinson SS. Imiterere y'uturemangingo tw'umubiri duto duto n'uruhare mu gutera ikibazo cy'ubudahangarwa bw'umubiri [J].J ya Clin Immunoassay, 1992,15:108-114.

Urufunguzo rw'ibimenyetso byakoreshejwe:

	Igikoresho cyo kwa muganga gisuzuma indwara muri Vitro
	Uruganda
	Bika ahantu hari ubushyuhe buri hagati ya 2 na 30°C
	Itariki izarangiriraho
	Ntukongere gukoresha
	UBWITONDE
	Reba amabwiriza yo gukoresha

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