Diagnostic Kit yeProgesterone (fluorescence immunochromatographic assay)
Diagnostic Kit yeProgesterone(fluorescence immunochromatographic assay)
Yekushandisa in vitro diagnostic chete
Ndokumbira uverenge iyi pasuru isa nekungwarira usati washandisa uye kunyatsotevera mirairo.Kuvimbika kwemhedzisiro yeassay hakugone kuvimbiswa kana paine chero kutsauka kubva kune mirairo mune ino pasuru yekuisa.
ZVIRI KUSHANDISA
Diagnostic Kit yeProgesterone (fluorescence immunochromatographic assay) inonzi fluorescence immunochromatographic assay yehuwandu hwekuonekwa kweProgesterone (PROG) muserum yevanhu kana plasma, inoshandiswa pakubatsira kuongororwa kweprogesterone zvirwere zvisingawanzobatanidzwa. .Muedzo uyu wakaitirwa kushandiswa nenyanzvi dzezvehutano chete.
SUMMARY
Progesterone ihomoni inokosha inoita basa rinokosha mukugadzirisa kwekuenda kumwedzi uye inokosha pakuchengetedza pamuviri.Kuwanda kweprogesterone mu serum kwakawedzera nekukurumidza mushure mekuvhara.Icho chiratidzo chinovimbika chezvakasikwa ovulation kana induction of ovulation.
ZVINOTAURWA ZVINOITA
Iyo membrane yechishandiso chekuyedza yakavharwa neiyo conjugate yeBSA uye PROG padunhu rekuyedza uye mbudzi anti tsuro IgG antibody padunhu rekutonga.Marker pad akaputirwa nefluorescence mark anti PROG antibody uye tsuro IgG pamberi.Paunenge uchiyedza sampuli, PROG mumuenzaniso unosanganisa nefluorescence yakanyorwa anti PROG antibody, uye gadzira immune musanganiswa.Pasi pechiito che immunochromatography, iyo yakaoma inoyerera ikananga kune pepa rinonyura, apo yakaoma yakapfuura dunhu rekuyedzwa, Iyo yakasununguka fluorescent marker ichabatanidzwa nePROG pa membrane.Kusangana kwePROG kunokanganisa kuwirirana kwechiratidzo chefluorescence, uye iyo kusangana kwePROG mumuenzaniso kunogona kuonekwa ne fluorescence immunoassay assay.
REAGENTS NEZVINHU ZVINOPIWA
25T package zvikamu:
Kadhi rekuyedza rimwe nerimwe foil rakaiswa muhomwe ine desiccant 25T
Sample diluents 25T
Package isa 1
ZVINHU ZVINODIWA ASI ZVISINA KUPIWA
Sample yekuunganidza mudziyo, timer
SAMPLE KUCHENGA UYE KUCHENGA
1.Mienzaniso yakaedzwa inogona kuva serum, heparin anticoagulant plasma kana EDTA anticoagulant plasma.
2.According to standard techniques unganidza sampuli.Serum kana plasma sampuli inogona kuchengetwa firiji pa 2-8 ℃ kwemazuva 7 uye cryopreservation pazasi -15 ° C kwemwedzi mitanhatu.
3.Yese sampuli inodzivirira kutonhora-kunyungudika kutenderera.
ASAY PROCEDURE
Ndokumbira uverenge bhuku rekushandisa chekushandisa uye pasuru isa usati waedza.
1.Kuisa parutivi zvose reagents uye samples kune tembiricha yekamuri.
2Vhura iyo Inotakurika Immune Analyzer (WIZ-A101), isa iyo account password login zvinoenderana nemashandiro echiridzwa, uye isa iyo yekuona interface.
3.Scan dentification code kuti usimbise chinhu chekuedza.
4.Bvisa kadhi rekuedza kubva mubhegi refoil.
5.Isai kadhi rekuedza mukadhi kadhi, tarisai QR code, uye sarudza chinhu chekuedza.
6.Wedzera 20μL serum kana plasma sampuli kuti sampuli diluent, uye sanganisa zvakanaka.
7.Wedzera 80μL sampuli mhinduro kuti uenzanise zvakanaka wekadhi.
8.Dzvanya bhatani re "standard test", mushure memaminitsi e10, chiridzwa chichangoona kadhi rekuedza, rinogona kuverenga migumisiro kubva pachiratidziro chechiridzwa chechiridzwa, uye nyora / kudhinda mhinduro dzekuedza.
9.Rejera kune rairo yePortable Immune Analyzer(WIZ-A101).
TEST RESULTS UYE DUDZIRIRO
| Danho | Range(ng/mL) | |
| Murume | 0.1-0.9 | |
| Mukadzi | follicular phase/ovulatory period | 0.3-1.5 |
| Luteal phase | 5.2-18.5 | |
| Kuenda kumwedzi | ＜0.8 | |
.Iyo data iri pamusoro apa mhedzisiro yePROG reagent test, uye zvinokurudzirwa kuti laboratori yega yega inofanirwa kumisa huwandu hwePROG yekuona kukosha kwakakodzera kune vanhu munharaunda ino.Mibairo iri pamusoro ndeyekutarisa chete.
.Migumisiro yeiyi nzira inoshandiswa chete kune zvinyorwa zvinotaridzirwa zvakasimbiswa nenzira iyi, uye hapana kuenzaniswa kwakananga nedzimwe nzira.
.Zvimwe zvikonzero zvinogonawo kukonzera kukanganisa mumigumisiro yekuona, kusanganisira zvikonzero zvehutano, zvikanganiso zvekushanda uye zvimwe zvinhu zvemuenzaniso.
KUCHENGA UYE KUgadzikana
1.Ikiti ndeye 18 mwedzi pasherufu-hupenyu kubva pazuva rekugadzirwa.Chengetedza makiti asina kushandiswa pa2-30°C.MUSAIZE.Usashandise kupfuura zuva rekupera.
2.Usavhura homwe yakavharwa kusvika wagadzirira kuita bvunzo, uye bvunzo-yekushandisa imwe chete inokurudzirwa kushandiswa pasi penzvimbo inodiwa (tembiricha 2-35 ℃, humidity 40-90%) mukati me60 mins nekukurumidza. sezvinobvira.
3 Sample diluent inoshandiswa pakarepo mushure mekuvhurwa.
YAMBIRO UYE CHENJERERO
.Ikiti inofanira kuvharwa uye kuchengetedzwa kubva pakunyorova.
.Yese mienzaniso yakanaka ichasimbiswa nedzimwe nzira.
.Mienzaniso yese ichabatwa sezvingango svibisa.
.USA shandisa expired reagent.
.USACHINANA mareagents pakati pemakiti ane akawanda akawanda Nha.
.USAshandise zvakare makadhi ebvunzo uye chero zvinhu zvinoraswa.
.Misoperation, yakawandisa kana sampuli shoma inogona kutungamirira kumhedzisiro yekutsauka.
LIMITATION
.Sezvinoita chero muyedzo unoshandisa masoja ekudzivirira embeva, mukana uripo wekukanganiswa nemasoja ekudzivirira mbeva (HAMA) mumuenzaniso.Mienzaniso kubva kuvarwere vakagamuchira gadziriro dzemonoclonal antibodies yekuongorora kana kurapwa inogona kunge iine HAMA.Mienzaniso yakadaro inogona kukonzera nhema dzakanaka kana nhema dzisina kunaka.
.Iyi mhedzisiro yebvunzo ndeyekuongororwa kwekiriniki chete, haifanire kushanda seyo chete hwaro hwekuongororwa kwekiriniki uye kurapwa, varwere vekiriniki manejimendi vanofanirwa kutarisisa kwakadzama kusanganiswa nezviratidzo zvayo, nhoroondo yezvekurapa, imwe ongororo yerabhoritari, mhinduro yekurapa, epidemiology uye mamwe mashoko. .
.Iri reagent rinoshandiswa chete serum uye plasma tests.Iyo inogona kusawana mhedzisiro chaiyo kana ichishandiswa kune mamwe masampuli senge mate uye weti uye nezvimwe.
PERFORMANCE CHARACTERISTICS
| Linearity | 0.5ng/mL kusvika 50ng/mL | hama kutsauka: -15% kusvika +15%. |
| Linear coefficient yekubatanidza:(r)≥0.9900 | ||
| Kururama | Chiyero chekudzorera chichave mukati me85% - 115%. | |
| Kudzokorora | CV≤15% | |
| Zvakananga(Hapana chimwe chezvinhu pane chakakanganiswa chakaedzwa chakakanganisa muyedzo) | Kupindira | Kuvhiringidza kuisa pfungwa |
| E2 | 500ng/mL | |
| T | 500ng/mL | |
| VaK | 500ng/mL | |
| E3 | 100ng/mL | |
| 17β-E2 | 100ng/mL | |
REFERENCES
1.Hansen JH, et al.HAMA Kupindira neMurine Monoclonal Antibody-Based Immunoassays[J].J yeClin Immunoassay,1993,16:294-299.
2.Levinson SS.Mamiriro eHeterophilic Antibodies uye Basa muImmunoassay Interference[J].J yeClin Immunoassay,1992,15:108-114.
Kiyi yezviratidzo zvakashandiswa:
 | In Vitro Diagnostic Medical Device |
 | Mugadziri |
 | Chengetedza pa2-30 ℃ |
 | Zuva rekupera |
 | Usashandisezve |
 | KUCHENJERA |
 | Bvunza Mirayiridzo Yekushandisa |
Xiamen Wiz Biotech CO., LTD
Kero:3-4 Floor,NO.16 Building,Bio-medical Workshop,2030 Wengjiao West Road,Haicang District,361026,Xiamen,China
Tel:+86-592-6808278
Fax:+86-592-6808279
