Igikoresho cyo gusuzuma kuri Progesterone(fluorescence immunochromatographic assay)
Kuri vitro kwisuzumisha koresha gusa
Nyamuneka soma iyi paki shyiramo witonze mbere yo gukoresha kandi ukurikize neza amabwiriza.Ibisubizo byizewe ntibishobora kwemezwa niba hari gutandukana kwamabwiriza muriyi paki yinjizamo.

UKORESHEJWE
Igikoresho cyo gusuzuma indwara ya Progesterone (fluorescence immunochromatographic assay) ni isuzuma rya fluorescence immunochromatographic assay kugirango hamenyekane umubare wa Progesterone (PROG) muri serumu yumuntu cyangwa plasma, ikoreshwa mugupima ubufasha bwindwara ya progesterone idasanzwe.Icyitegererezo cyiza cyose kigomba kwemezwa nubundi buryo .Iki kizamini kigenewe ubuvuzi bwumwuga gusa.

INCAMAKE
Progesterone ni imisemburo ikomeye igira uruhare runini mugutunganya imihango kandi ni ngombwa mugukomeza gutwita.Ubunini bwa progesterone muri serumu bwiyongereye vuba nyuma yintanga.Nibimenyetso byizewe byerekana intanga ngabo cyangwa kwinjiza intanga ngabo.

IHame RY'UBURYO
Ikibumbano cyibikoresho byipimishije gishyizwe hamwe na conjugate ya BSA na PROG mukarere kizamini hamwe na ihene irwanya urukwavu IgG antibody mukarere kayobora.Ikimenyetso cya marikeri gitwikiriwe na fluorescence ikimenyetso kirwanya antibody ya PROG ninkwavu IgG mbere.Iyo ugerageza icyitegererezo, PROG muri sample ikomatanya na fluorescence yanditseho antibody ya PROG, hanyuma igakora imvange yumubiri.Mubikorwa byubudahangarwa bw'umubiri, urujya n'uruza rugana mu cyerekezo cy'impapuro zinjira, iyo complexe yatsinze akarere k'ibizamini, Ikimenyetso cya fluorescent yubusa kizahuzwa na PROG kuri membrane. Kwibanda kwa PROG ni ihuriro ribi ryerekana ibimenyetso bya fluorescence, na kwibanda kuri PROG murugero birashobora gutahurwa na fluorescence immunoassay assay.

REAGENTS N'IBIKORWA BYUZUWE
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IBIKORWA BISABWA ARIKO NTIBITANZWE
Icyitegererezo cyo gukusanya ibikoresho, igihe

GUKORANYA URUGERO N'UBubiko
1.Ingero zapimwe zishobora kuba serumu, heparin anticoagulant plasma cyangwa plasma ya EDTA anticoagulant.
2.Kurikije tekiniki zisanzwe zegeranya icyitegererezo.Icyitegererezo cya serumu cyangwa plasma birashobora kubikwa muri firigo kuri 2-8 ℃ muminsi 7 hamwe no kubika munsi -15 ° C mumezi 6.
3.Icyitegererezo cyose wirinde gukonjesha.

GUKORA UBURYO
Nyamuneka soma igitabo gikoreshwa nigikoresho cyo gushyiramo mbere yo kugerageza.
1. Shira ku ruhande reagent zose hamwe nicyitegererezo kubushyuhe bwicyumba.
2 Fungura Portable Immune Analyser (WIZ-A101), andika ijambo ryibanga rya konte ukurikije uburyo bwo gukora igikoresho, hanyuma winjire muburyo bwo kumenya.
3.Shobora kode ya dentification kugirango wemeze ikintu cyizamini.
4.Kuramo ikarita yikizamini mu mufuka wa file.
5. Shyiramo ikarita yikizamini ahantu h'ikarita, suzuma kode ya QR, hanyuma umenye ikintu cyizamini.
6. Ongeramo 20μL serumu cyangwa plasma icyitegererezo kugirango utange urugero, hanyuma uvange neza ..
7. Ongeraho 80μL igisubizo cyicyitegererezo kugirango utange neza ikarita.
8.Kanda buto ya "test test", nyuma yiminota 10, igikoresho kizahita kimenya ikarita yikizamini, kirashobora gusoma ibisubizo bivuye mugice cyo kwerekana igikoresho, hanyuma wandike / wandike ibisubizo byikizamini.
9. Reba ku mabwiriza ya Portable Immune Analyser (WIZ-A101).

IBISUBIZO BY'IKIZAMINI NO GUSOBANURA

.Amakuru yavuzwe haruguru ni ibisubizo byikizamini cya PROG reagent, kandi hasabwa ko buri laboratoire igomba gushyiraho indangagaciro zerekana PROG zibereye abaturage muri kano karere.Ibisubizo byavuzwe haruguru nibisobanuro gusa.
.Ibisubizo byubu buryo birakoreshwa gusa murwego rwoherejwe rwashyizweho muri ubu buryo, kandi ntaho bihuriye nubundi buryo.
.Ibindi bintu birashobora kandi gutera amakosa mubisubizo byo gutahura, harimo impamvu za tekiniki, amakosa yimikorere nibindi bintu byintangarugero.

Ububiko N'UBUHAMYA
1.Ibikoresho ni amezi 18 yo kubaho-uhereye igihe byakorewe.Bika ibikoresho bidakoreshwa kuri 2-30 ° C.NTUBUNTU.Ntukoreshe kurenza itariki izarangiriraho.
2.Ntukingure umufuka ufunze kugeza igihe witeguye gukora ikizamini, kandi ikizamini kimwe cyo gukoreshwa gisabwa gukoreshwa mubisabwa (ubushyuhe 2-35 ℃, ubuhehere 40-90%) muminota 60 byihuse. bishoboka.
3Urugero rwa diluent rukoreshwa ako kanya nyuma yo gufungura.

UMUBURO N'UBWITONDERWA
.Ibikoresho bigomba gufungwa kandi bikarindwa ubushuhe.
.Ingero zose nziza zemezwa nubundi buryo.
.Ingero zose zifatwa nkibishobora guhumanya.
.Ntugakoreshe reagent yarangiye.
.NtUGASIMBURE reagent mubikoresho bifite ubufindo butandukanye Oya ..
.NtUGASUBIZE gukoresha amakarita yikizamini nibikoresho byose bikoreshwa.
.Gukoresha nabi, urugero rwinshi cyangwa ruto rushobora kuganisha ku gutandukana.

LKwigana
.Nkuko hamwe nubushakashatsi ubwo aribwo bwose bukoresha antibodi yimbeba, birashoboka ko habaho kwivanga na antibodiyite zabantu barwanya imbeba (HAMA) murugero.Ingero z'abarwayi bahawe imyiteguro ya antibodiyite ya monoclonal yo gusuzuma cyangwa kuvura irashobora kuba irimo HAMA.Ingero nkizo zishobora gutera ibinyoma byiza cyangwa ibinyoma bibi.
.Ibisubizo by'ibizamini ni ibyerekeye ivuriro gusa, ntibigomba kuba ishingiro ryonyine ryo gusuzuma no kuvura kwa muganga, imicungire y’amavuriro y’abarwayi igomba kwitabwaho byimazeyo hamwe n’ibimenyetso byayo, amateka y’ubuvuzi, ibindi bizamini bya laboratoire, igisubizo cy’ubuvuzi, epidemiologiya nandi makuru .
.Iyi reagent ikoreshwa gusa mugupima serumu na plasma.Ntishobora kubona ibisubizo nyabyo mugihe ikoreshejwe izindi ngero nka macandwe ninkari nibindi.

IBIKORWA BIKORWA

RINGARUKA
1.Hansen JH, n'abandi.HAMA Kwivanga kwa Murine Monoclonal Antibody ishingiye kuri Immunoassays [J] .J ya Clin Immunoassay, 1993,16: 294-299.
2.Levinson S.

Urufunguzo rwibimenyetso byakoreshejwe:

	Mubikoresho byubuvuzi bya Vitro
	Uruganda
	Ubike kuri 2-30 ℃
	Itariki izarangiriraho
	Ntugakoreshe
	ICYITONDERWA
	Baza Amabwiriza yo Gukoresha

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