Igikoresho cyo gusuzuma kuri testosterone(fluorescence immunochromatographic assay)
Kuri vitro kwisuzumisha koresha gusa
Nyamuneka soma iyi paki shyiramo witonze mbere yo gukoresha kandi ukurikize neza amabwiriza.Ibisubizo byizewe ntibishobora kwemezwa niba hari gutandukana kwamabwiriza muriyi paki yinjizamo.

UKORESHEJWE
Igikoresho cyo kwisuzumisha kuri testosterone (fluorescence immunochromatographic assay) ni fluorescence immunochromatographic assay kugirango igaragaze umubare wa Testosterone (T) muri serumu yumuntu cyangwa plasma, ikoreshwa cyane mugusuzuma urwego rwa testosterone.Ni reagent yo kwisuzumisha. bigomba kwemezwa nubundi buryo.Iki kizamini kigenewe ubuvuzi bwumwuga gusa.

INCAMAKE
Testosterone (T), ifite uburemere bwa 288.4 D. Muri sisitemu yo gutembera, hafi 97% kugeza 99% bya testosterone ihuza poroteyine za plasma. Muri bo, 60% ya testosterone mu maraso iba ihujwe na hormone yimibonano mpuzabitsina ihuza globuline ( SHBG), 38% byanze bikunze albumin, naho 2% ni ubuntu.Guhuza testosterone mu maraso ntabwo ikora mubinyabuzima, kandi testosterone yubusa niyo ishobora kwinjira muri selile ya rake kugirango ikore ingaruka zumubiri.

IHame RY'UBURYO
Ibice by'igikoresho cyo kwipimisha bisizwe hamwe na conjugate ya BSA na Testosterone mu karere k'ibizamini hamwe na antibody y'ihene irwanya urukwavu IgG mu karere kayobora.Ikimenyetso cya marike gitwikiriwe na fluorescence ikimenyetso kirwanya antibody ya testosterone hamwe ninkwavu IgG mbere.Mugihe cyo gupima icyitegererezo, Testosterone murugero ikomatanya na fluorescence yaranze antibody ya testosterone, hanyuma igakora imvange yumubiri.Mubikorwa bya immunochromatografiya, urujya n'uruza rugana mu cyerekezo cyimpapuro zinjira, mugihe complexe yatsinze akarere kizamini, Ikimenyetso cya fluorescent yubusa kizahuzwa na Testosterone kuri membrane.Ubushuhe bwa Testosterone ni isano ribi kubimenyetso bya fluorescence, na kwibanda kwa Testosterone murugero birashobora gutahurwa na fluorescence immunoassay assay.

REAGENTS N'IBIKORWA BYUZUWE

25T ibice：
.Ikarita yipimisha kugiti cyawe ifu yometse kuri desiccant 25T
.Umuti 25T
.B igisubizo 1
.Paki yinjizamo 1

IBIKORWA BISABWA ARIKO NTIBITANZWE
Icyitegererezo cyo gukusanya ibikoresho, igihe

GUKORANYA URUGERO N'UBubiko
1.Ingero zapimwe zishobora kuba serumu, heparin anticoagulant plasma cyangwa plasma ya EDTA anticoagulant.
2.Kurikije tekiniki zisanzwe zegeranya icyitegererezo.Icyitegererezo cya serumu cyangwa plasma birashobora kubikwa muri firigo kuri 2-8 ℃ muminsi 7 hamwe no kubika munsi -15 ° C mumezi 6.
3.Icyitegererezo cyose wirinde gukonjesha.

GUKORA UBURYO
Uburyo bwo gupima igikoresho reba igitabo gikingira immunoanalyser.Uburyo bwo kwipimisha reagent nuburyo bukurikira
1. Shira ku ruhande reagent zose hamwe nicyitegererezo kubushyuhe bwicyumba.
2.Fungura Portable Immune Analyser (WIZ-A101), andika ijambo ryibanga rya konte ukurikije uburyo bukoreshwa bwigikoresho, hanyuma winjire muburyo bwo kumenya.
3.Shobora kode ya dentification kugirango wemeze ikintu cyizamini.
4.Kuramo ikarita yikizamini mu mufuka wa file.
5. Shyiramo ikarita yikizamini ahantu h'ikarita, suzuma kode ya QR, hanyuma umenye ikintu cyizamini.
6. Ongeramo 30μL serumu cyangwa plasma icyitegererezo kubisubizo, hanyuma uvange neza.
7. Ongeraho 20μL B igisubizo kivanze hejuru, hanyuma uvange neza.
8. Kureka imvange muminota 20.
9. Ongeramo 80μL ivanze kugirango utange neza ikarita.
10.Kanda buto ya "test test", nyuma yiminota 10, igikoresho kizahita kimenya ikarita yikizamini, kirashobora gusoma ibisubizo bivuye mugice cyo kwerekana igikoresho, hanyuma wandike / wandike ibisubizo byikizamini.
11. Reba ku mabwiriza ya Portable Immune Analyser (WIZ-A101).

AGACIRO KATEGANYIJWE

Urwego rusanzwe rwa testosterone: Umugabo: 2.5-10.5ng / mL
Umugore: 0.25-1.0ng / mL
Birasabwa ko buri laboratoire ishyiraho urwego rwayo rusanzwe ruhagarariye abaturage barwaye.

IBISUBIZO BY'IKIZAMINI NO GUSOBANURA
.Amakuru yavuzwe haruguru ni intera yerekanwe yashyizweho kugirango hamenyekane amakuru yiki gitabo, kandi birasabwa ko buri laboratoire igomba gushyiraho intera yerekana akamaro k’amavuriro y’abaturage muri kano karere.
.Ubushuhe bwa Testosterone burenze urwego ruvugwa, kandi impinduka za physiologique cyangwa igisubizo cyibibazo bigomba kuvaho.Ibidasanzwe bidasanzwe, bigomba guhuza gusuzuma ibimenyetso byindwara.
.Ibisubizo byubu buryo birakoreshwa gusa murwego rwoherejwe rwashyizweho nubu buryo, kandi ibisubizo ntabwo bigereranywa nubundi buryo.
.Ibindi bintu birashobora kandi gutera amakosa mubisubizo byo gutahura, harimo impamvu za tekiniki, amakosa yimikorere nibindi bintu byintangarugero.

Ububiko N'UBUHAMYA
1.Ibikoresho ni amezi 18 yo kubaho-uhereye igihe byakorewe.Bika ibikoresho bidakoreshwa kuri 2-30 ° C.NTUBUNTU.Ntukoreshe kurenza itariki izarangiriraho.
2.Ntukingure umufuka ufunze kugeza igihe witeguye gukora ikizamini, kandi ikizamini kimwe cyo gukoreshwa gisabwa gukoreshwa mubisabwa (ubushyuhe 2-35 ℃, ubuhehere 40-90%) muminota 60 byihuse. bishoboka.
3.Urugero rwa diluent rukoreshwa ako kanya nyuma yo gufungura.

UMUBURO N'UBWITONDERWA
.Ibikoresho bigomba gufungwa kandi bikarindwa ubushuhe.
.Ingero zose nziza zemezwa nubundi buryo.
.Ingero zose zifatwa nkibishobora guhumanya.
.Ntugakoreshe reagent yarangiye.
.NtUGASIMBURE reagent mubikoresho bifite ubufindo butandukanye oya ..
.NtUGASUBIZE gukoresha amakarita yikizamini nibikoresho byose bikoreshwa.
.Gukoresha nabi, urugero rwinshi cyangwa ruto rushobora kuganisha ku gutandukana.

LKwigana
.Nkuko hamwe nubushakashatsi ubwo aribwo bwose bukoresha antibodi yimbeba, birashoboka ko habaho kwivanga na antibodiyite zabantu barwanya imbeba (HAMA) murugero.Ingero z'abarwayi bahawe imyiteguro ya antibodiyite ya monoclonal yo gusuzuma cyangwa kuvura irashobora kuba irimo HAMA.Ingero nkizo zishobora gutera ibinyoma byiza cyangwa ibinyoma bibi.
.Ibisubizo by'ibizamini ni ibyerekeye ivuriro gusa, ntibigomba kuba ishingiro ryonyine ryo gusuzuma no kuvura kwa muganga, imicungire y’amavuriro y’abarwayi igomba kwitabwaho byimazeyo hamwe n’ibimenyetso byayo, amateka y’ubuvuzi, ibindi bizamini bya laboratoire, igisubizo cy’ubuvuzi, epidemiologiya nandi makuru .
.Iyi reagent ikoreshwa gusa mugupima serumu na plasma.Ntishobora kubona ibisubizo nyabyo mugihe ikoreshejwe izindi ngero nka macandwe ninkari nibindi.

IBIKORWA BIKORWA

INGINGO
1.Hansen JH, n'abandi.HAMA Kwivanga kwa Murine Monoclonal Antibody ishingiye kuri Immunoassays [J] .J ya Clin Immunoassay, 1993,16: 294-299.
2.Levinson S.

Urufunguzo rwibimenyetso byakoreshejwe:

	Mubikoresho byubuvuzi bya Vitro
	Uruganda
	Ubike kuri 2-30 ℃
	Itariki izarangiriraho
	Ntugakoreshe
	ICYITONDERWA
	Baza Amabwiriza yo Gukoresha

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