Kete Tātaritanga mō te Progesterone(whakamātautau ārai-ira-whakaahua-whakaahua)
Mō te whakamahinga tātaritanga in vitro anake
Pānuihia tēnei tāpiringa i mua i te whakamahinga, ā, me whai pūmau i ngā tohutohu. Kāore e taea te whakamana i te pono o ngā hua whakamātautau mēnā he rerekētanga mai i ngā tohutohu kei roto i tēnei tāpiringa.

TE WHAKAMAHINGA I WHAKATAURANGIA
Ko te Kete Tātaritanga mō te Progesterone (whakamātautau immunochromatographic fluorescence) he whakamātautau immunochromatographic fluorescence mō te kimi i te Progesterone (PROG) i roto i te toto tangata, i te plasma rānei, e whakamahia ana mō te tātaritanga āwhina o ngā mate e pā ana ki te progesterone. Me whakaū te tauira pai katoa mā ētahi atu tikanga. Ko tēnei whakamātautau he mea hanga mō te whakamahinga hauora anake.

WHAKARĀPOPOTONGA
He homoni nui te progesterone e whai wāhi nui ana ki te whakahaere i te huringa paheketanga, ā, he mea nui hoki ki te pupuri i te haputanga. Ka tere te piki o te kukū o te progesterone i roto i te toto i muri i te whakaputanga o te hua manu. He tohu pono tēnei mō te whakaputanga o te hua manu maori, te whakaoho rānei i te whakaputanga o te hua manu.

TE MĀTĀPONO O TE TIKANGA
Kua pania te kiriuhi o te taputapu whakamātautau ki te hononga o te BSA me te PROG i te rohe whakamātautau me te paturopi IgG ārai rāpeti koati i te rohe whakahaere. Kua pania ngā papa tohu ki te tohu fluorescence paturopi anti PROG me te IgG rāpeti i mua. I te whakamātautau i te tauira, ka honoa te PROG i roto i te tauira ki te paturopi anti PROG kua tohua ki te fluorescence, ā, ka hanga he ranunga ārai mate. I raro i te mahi a te immunochromatography, ka rere te matatini i te ahunga o te pepa mimiti, ina paahitia e te matatini te rohe whakamātautau, ka honoa te tohu fluorescence kore utu ki te PROG i runga i te kiriuhi. He hononga kino te kukū o te PROG mō te tohu fluorescence, ā, ka taea te kite i te kukū o te PROG i roto i te tauira mā te whakamātautau immunoassay fluorescence.

NGĀ RĀWENI ME NGĀ RAUEMI I TUKUNA
Ngā wāhanga mōkihi 25T：
Kāri whakamātautau takitahi, he mea takai ki te pepa konutea me te whakamaroke 25T
Ngā wairewa tauira 25T
Tāpiringa mōkihi 1

NGĀ RAUEMI E HIAHIATIA ANA ENGARI KĀORE I HOATU
Ipu kohinga tauira, taima

TE KOHINGA TAUIRA ME TE ROKIROKI
1. Ko ngā tauira i whakamatautauria he toto toto, he toto toto heparin anticoagulant, he toto toto EDTA anticoagulant rānei.
2. E ai ki ngā tikanga paerewa, kohia he tauira. Ka taea te pupuri i te tauira toto, i te tauira plasma rānei ki roto i te pouaka whakamātao i te 2-8℃ mō ngā rā e 7, ā, ka taea te pupuri i te tauira i raro i te -15°C mō ngā marama e 6.
3. Kaua ngā tauira katoa e huri i te huka me te rewa.

TE TIKANGA WHAKAMĀTAUTAU
Pānuihia te pukapuka whakahaere taputapu me te tāpiringa ki te kete i mua i te whakamātautau.
1. Waiho ngā matū me ngā tauira katoa ki te pāmahana rūma.
2Whakatuwheratia te Pūnaha Tātari Ārai Mate Kawe (WIZ-A101), tāuruhia te kupuhipa takiuru pūkete e ai ki te tikanga whakahaere o te taputapu, ka tomo ki te atanga kimi.
3. Matawaihia te waehere whakaū niho hei whakaū i te tūemi whakamātautau.
4. Tangohia te kāri whakamātautau i te putea pepa konutea.
5. Whakauruhia te kāri whakamātautau ki roto i te mokamoka kāri, matawaihia te waehere QR, ka kimihia te tūemi whakamātautau.
6. Tāpirihia he 20μL o te tauira toto, te tauira plasma rānei ki te wai whakawera tauira, ā, whakaranua kia pai.
7. Tāpirihia he 80μL o te otinga tauira ki te puna tauira o te kāri.
8. Pāwhiritia te pātene "whakamātautau paerewa", i muri i te 10 meneti, ka kitea aunoa e te taputapu te kāri whakamātautau, ka taea te pānui i ngā hua mai i te mata whakaatu o te taputapu, ā, ka tuhia/tāia ngā hua whakamātautau.
9. Tirohia ngā tohutohu mō te Pūnaha Tātari Ārai Mate Kawe (WIZ-A101).

NGĀ HUA WHAKAMĀTAUTAU ME TE WHAKAMĀRAMATANGA

Ko ngā raraunga o runga ake nei he hua nō te whakamātautau matū PROG, ā, e kīia ana me whakatū e ia taiwhanga he whānuitanga o ngā uara kitenga PROG e tika ana mō te taupori o tēnei rohe. Hei tohutoro noa iho ngā hua o runga ake nei.
Ko ngā hua o tēnei tikanga ka pā anake ki ngā awhe tohutoro kua whakatūria i roto i tēnei tikanga, ā, kāore he whakataurite tika ki ētahi atu tikanga.
Ka taea hoki e ētahi atu āhuatanga te whakaputa hapa i ngā hua kimi, tae atu ki ngā take hangarau, ngā hapa whakahaere me ētahi atu āhuatanga tauira.

TE ROKIROKI ME TE PŪMAU
1. E 18 marama te roa o te oranga o te kete mai i te rā i hangaia ai. Penapenahia ngā kete kāore anō kia whakamahia ki te 2-30°C. KAUA E WHAKATIO. Kaua e whakamahia i tua atu i te rā paunga.
2. Kaua e huakina te putea hiri kia rite rā anō koe ki te mahi i tētahi whakamātautau, ā, e taunakihia ana kia whakamahia te whakamātautau kotahi-whakamahinga i raro i te taiao e hiahiatia ana (pāmahana 2-35℃, haumākū 40-90%) i roto i te 60 meneti i te wā tere rawa atu.
3Ka whakamahia te wai whakawera tauira i muri tonu i te whakatuwheratanga.

NGĀ WHAKATŪPATO ME NGĀ WHAKATŪPATO
Me hiri te kete, ā, me tiaki hoki i te makuku.
Me whakamana ngā tauira pai katoa mā ētahi atu tikanga.
Me whakaaro ngā tauira katoa he poke pea.
KAUA e whakamahia te matū kua pau te wā.
.KAUA e whakawhiti i ngā matū matū i waenga i ngā kete he rerekē te nama puranga.
KAUA e whakamahi anō i ngā kāri whakamātautau me ngā taputapu whakakorekore.
.Mēnā he te whakamahinga, he nui rawa, he iti rānei te tauira, ka rerekē pea ngā hua.

LWHAKATAURANGA
Pērā i ngā whakamātautau katoa e whakamahi ana i ngā paturopi kiore, tera pea ka pāngia te tauira e ngā paturopi ārai-kiore tangata (HAMA). Tērā pea kei roto i ngā tauira mai i ngā tūroro kua whiwhi i ngā whakaritenga o ngā paturopi monoclonal mō te tātaritanga, te rongoā rānei, te HAMA. Tērā pea ka puta he hua hē, he hua hē rānei.
Hei tohutoro haumanu anake tēnei hua whakamātautau, kaua e waiho hei pūtake anake mō te tātaritanga haumanu me te maimoatanga, me whai whakaaro whānui te whakahaere haumanu o ngā tūroro me ōna tohu, hītori hauora, ētahi atu whakamātautau taiwhanga, urupare maimoatanga, mate urutā me ētahi atu mōhiohio.
Ka whakamahia anake tēnei matū hei whakamātautau i te toto me te toto toto. Tērā pea kāore e tika te hua ina whakamahia mō ētahi atu tauira pēnei i te huware me te mimi me ētahi atu.

NGĀ ĀHUATANGA MAHI

RNGĀ WHAKAMĀRAMA
1.Hansen JH, et al. Te Whakararuraru HAMA ki ngā Immunoassays Murine Monoclonal Antibody-Based Immunoassays[J].J o Clin Immunoassay,1993,16:294-299.
2.Levinson SS. Te Āhua o ngā Paturopi Heterophilic me te Tūranga i roto i te Whakararuraru Immunoassay[J].J o Clin Immunoassay,1992,15:108-114.

Ngā tohu matua i whakamahia:

	Pūrere Hauora Tātaritanga In Vitro
	Kaihanga
	Penapena ki te 2-30℃
	Rā Paunga
	Kaua e Whakamahi Anō
	WHAKATŪPATO
	Tirohia ngā Tohutohu Whakamahinga

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