Cov Khoom Siv Kuaj Mob rau Tag Nrho Thyroxine (Kev ntsuam xyuas fluorescence immunochromatographic)
Rau kev siv kuaj mob hauv vitro xwb
Thov nyeem daim ntawv qhia no kom zoo ua ntej siv thiab ua raws li cov lus qhia. Kev ntseeg tau ntawm cov txiaj ntsig ntawm kev sim tsis tuaj yeem lav yog tias muaj kev hloov pauv ntawm cov lus qhia hauv daim ntawv qhia no.

YUAV TSUM SIV LI CAS
Cov Khoom Siv Kuaj Mob rau Tag Nrho Thyroxine (fluorescence immunochromatographic assay) yog qhov kev ntsuam xyuas fluorescence immunochromatographic rau qhov ntau ntawm Total Thyroxine (TT4) hauv tib neeg cov ntshav lossis cov ntshav, uas feem ntau yog siv los ntsuam xyuas cov haujlwm ntawm thyroid. Nws yog ib qho tshuaj reagent kuaj mob pabcuam. Txhua tus qauv zoo yuav tsum tau lees paub los ntawm lwm txoj kev. Qhov kev xeem no yog rau kev siv kho mob xwb.

LUS QHIA
Thyroxine (T4) yog secreted los ntawm lub thyroid caj pas thiab nws qhov hnyav molecular yog 777D. Tag nrho T4 (Tag Nrho T4, TT4) hauv cov ntshav yog 50 npaug ntawm cov ntshav T3. Ntawm lawv, 99.9% ntawm TT4 khi rau cov ntshav Thyroxine Binding Proteins (TBP), thiab dawb T4 (Free T4, FT4) yog tsawg dua 0.05%. T4 thiab T3 koom nrog kev tswj hwm lub cev metabolic function. TT4 kev ntsuas yog siv los ntsuas qhov xwm txheej ua haujlwm ntawm thyroid thiab kev kuaj mob ntawm cov kab mob. Clinically, TT4 yog ib qho cim qhia tau zoo rau kev kuaj mob thiab kev soj ntsuam kev ua haujlwm ntawm hyperthyroidism thiab hypothyroidism.

TSAB NTAWV QHIA TXOG TXHEEJ TXHEEM
Lub membrane ntawm lub cuab yeej kuaj tau coated nrog conjugate ntawm BSA thiab T4 ntawm thaj chaw kuaj thiab tshis tiv thaiv luav IgG antibody ntawm thaj chaw tswj. Lub cim ncoo tau coated los ntawm fluorescence cim anti T4 antibody thiab luav IgG ua ntej. Thaum kuaj cov qauv, TT4 hauv cov qauv sib xyaw nrog fluorescence cim anti T4 antibody, thiab tsim cov tshuaj tiv thaiv kab mob. Nyob rau hauv qhov kev ua ntawm immunochromatography, cov dej ntws hauv qhov kev taw qhia ntawm daim ntawv nqus, thaum complex dhau thaj chaw kuaj, Lub cim fluorescent dawb yuav raug ua ke nrog T4 ntawm daim nyias nyias. Qhov concentration ntawm TT4 yog qhov tsis zoo rau lub teeb liab fluorescence, thiab qhov concentration ntawm TT4 hauv cov qauv tuaj yeem pom los ntawm fluorescence immunoassay assay.

COV REAGENTS THIAB COV KHOOM SIV

Cov khoom siv pob khoom 25T：
.Test daim ntawv ib lub zuj zus ntawv ci pouched nrog ib tug desiccant 25T
.Ib qho kev daws teeb meem 25T
.B kev daws teeb meem 1
.Daim ntawv ntxig rau pob khoom 1

COV KHOOM SIV YUAV TSUM TAU TAB SIS TSIS TAU MUAB
Lub thawv sau qauv, lub sijhawm

KEV SAIB XYUAS THIAB CIA COV QAUV
1. Cov qauv kuaj tuaj yeem yog serum, heparin anticoagulant plasma lossis EDTA anticoagulant plasma.
2. Raws li cov txheej txheem txheem, sau cov qauv. Cov qauv ntshav lossis cov ntshav tuaj yeem khaws cia rau hauv lub tub yees ntawm 2-8 ℃ rau 7 hnub thiab cryopreservation hauv qab -15 ° C rau 6 lub hlis.
3. Tag nrho cov qauv tsis txhob khov thiab yaj.

TXHEEJ TXHEEM KEV NTSUAM XYUAS

Cov txheej txheem sim ntawm cov cuab yeej saib phau ntawv qhia immunoanalyzer. Cov txheej txheem sim reagent yog raws li nram no
1. Tso tag nrho cov tshuaj reagents thiab cov qauv rau qhov kub thiab txias hauv chav.
2. Qhib lub Portable Immune Analyzer (WIZ-A101), sau tus password nkag mus rau hauv tus account raws li txoj kev ua haujlwm ntawm lub cuab yeej, thiab nkag mus rau qhov interface nrhiav pom.
3. Luam theej daim code dentification kom paub meej tias yog yam khoom xeem.
3. Tshem daim ntawv xeem ntawm lub hnab ntawv ci.
4. Muab daim npav xeem tso rau hauv qhov chaw tso daim npav, luam theej daim QR code, thiab txiav txim siab seb yam khoom xeem yog dab tsi.
5. Ntxiv 20μL serum lossis plasma qauv rau A kua, thiab sib tov kom zoo.
6. Ntxiv 20μL B kua rau hauv cov sib tov saum toj no, thiab sib tov kom zoo.

Cia cov sib tov rau20feeb.
Ntxiv 80μL sib tov rau hauv qhov dej ntawm daim npav.
Nyem lub pob "kev xeem txheem", tom qab 10 feeb, lub cuab yeej yuav ntes tau daim npav xeem, nws tuaj yeem nyeem cov txiaj ntsig los ntawm qhov screen zaub ntawm lub cuab yeej, thiab sau / luam cov txiaj ntsig xeem.
Saib cov lus qhia ntawm Portable Immune Analyzer (WIZ-A101).

TUS NQI XAV TAU
TT4 ib txwm muaj: 55-140nmol/L
Nws raug pom zoo kom txhua lub chaw kuaj mob tsim nws tus kheej qhov ntau thiab tsawg ib txwm sawv cev rau nws cov neeg mob.

COV TXIAJ NTSUAM XYUAS THIAB KEV TXHAIS LUS
. Cov ntaub ntawv saum toj no yog qhov siv tau ntev uas tau tsim los rau cov ntaub ntawv nrhiav pom ntawm cov khoom siv no, thiab nws tau hais tias txhua lub chaw kuaj mob yuav tsum tsim kom muaj qhov siv tau ntev rau qhov tseem ceeb ntawm cov pej xeem hauv thaj av no.
.Qhov concentration ntawm TT4 siab dua qhov ntau thiab tsawg siv, thiab yuav tsum tsis txhob muaj kev hloov pauv ntawm lub cev lossis kev ntxhov siab. Tseeb tiag, yuav tsum muab cov tsos mob kuaj mob ua ke.
.Cov txiaj ntsig ntawm txoj kev no tsuas yog siv tau rau qhov ntau yam uas tau tsim los ntawm txoj kev no, thiab cov txiaj ntsig tsis sib piv ncaj qha nrog lwm txoj kev.
Lwm yam tseem tuaj yeem ua rau muaj qhov yuam kev hauv cov txiaj ntsig nrhiav tau, suav nrog cov laj thawj kev siv tshuab, kev ua haujlwm yuam kev thiab lwm yam piv txwv.

CIA THIAB RUAJ NTSEG
. Cov khoom siv no muaj 18 lub hlis txij li hnub tsim khoom. Khaws cov khoom siv tsis tau siv ntawm 2-30 ° C. TSIS TXHOB KHOV. Tsis txhob siv dhau hnub tas sij hawm.
.Tsis txhob qhib lub hnab kaw kom txog thaum koj npaj txhij los ua qhov kev sim, thiab qhov kev sim ib zaug siv tau raug pom zoo kom siv rau hauv qhov chaw xav tau (kub 2-35 ℃, av noo 40-90%) hauv 60 feeb sai li sai tau.
.Sample diluent siv tam sim ntawd tom qab qhib.

COV LUS CEEV FAIB THIAB COV KEV CEEB TOOM
. Cov khoom siv yuav tsum tau kaw thiab tiv thaiv kom tsis txhob muaj dej noo.
Txhua yam qauv zoo yuav tsum tau lees paub los ntawm lwm txoj kev.
Txhua yam qauv yuav tsum raug kho raws li cov kuab paug.
TSIS TXHOB siv cov tshuaj reagent uas tas sij hawm lawm.
.TSIS TXHOB pauv cov tshuaj reagents ntawm cov khoom siv nrog ntau yam tsis muaj.
TSIS TXHOB siv daim npav xeem thiab cov khoom siv pov tseg dua.
Kev ua haujlwm tsis raug, ntau dhau lossis me me tuaj yeem ua rau muaj kev hloov pauv ntawm cov txiaj ntsig.

LKEV UA YEES
Ib yam li kev sim siv cov tshuaj tua kab mob nas, qhov ua tau tias yuav muaj kev cuam tshuam los ntawm cov tshuaj tua kab mob nas tib neeg (HAMA) hauv cov qauv kuaj. Cov qauv kuaj los ntawm cov neeg mob uas tau txais kev npaj ntawm cov tshuaj monoclonal rau kev kuaj mob lossis kev kho mob yuav muaj HAMA. Cov qauv kuaj no yuav ua rau muaj qhov tshwm sim tsis zoo lossis tsis zoo.
.Qhov kev xeem no tsuas yog rau kev siv hauv kev kho mob xwb, yuav tsum tsis txhob ua lub hauv paus rau kev kuaj mob thiab kev kho mob xwb, kev tswj hwm kev kho mob ntawm cov neeg mob yuav tsum tau txiav txim siab ua ke nrog nws cov tsos mob, keeb kwm kho mob, lwm yam kev kuaj mob hauv chaw kuaj mob, kev kho mob teb, kev kis kab mob thiab lwm yam ntaub ntawv.
Cov tshuaj reagent no tsuas yog siv rau kev kuaj ntshav thiab ntshav xwb. Nws yuav tsis tau txais qhov tshwm sim tseeb thaum siv rau lwm cov qauv xws li cov qaub ncaug thiab zis thiab lwm yam.

COV CIM TXOG KEV UA HAUJ LWM

RCOV CIM XAV TAU
1.Hansen JH, thiab lwm tus.HAMA Kev Cuam Tshuam nrog Murine Monoclonal Antibody-Based Immunoassays [J].J ntawm Clin Immunoassay, 1993,16: 294-299.
2.Levinson SS.Qhov Xwm Txheej ntawm Heterophilic Antibodies thiab Lub Luag Haujlwm hauv Immunoassay Interference [J].J ntawm Clin Immunoassay, 1992,15: 108-114.

Tus yuam sij rau cov cim siv:

	Cov Khoom Siv Kho Mob Hauv Vitro Diagnostic
	Chaw Tsim Khoom
	Khaws cia ntawm 2-30 ℃
	Hnub tas sij hawm
	Tsis txhob siv dua
	CEEB TOOM
	Saib Cov Lus Qhia Txog Kev Siv

Xiamen Wiz Biotech CO., LTD
Chaw Nyob: 3-4 Pem Teb, NO.16 Tsev, Bio-medical Rhiav, 2030 Wengjiao West Road, Haicang District, 361026, Xiamen, Suav Teb
Xov tooj: + 86-592-6808278
Fax: + 86-592-6808279