Ikhithi Yokuxilonga ye-Procalcitonin
Ikhithi Yokuxilonga ye-Cardiac Troponin I ∕Isoenzyme MB ye-Creatine Kinase ∕Myoglobin
Indlela Yokusebenza: Ukuhlolwa Kwe-Fluorescence Immunochromatographic
Ulwazi lokukhiqiza
| Inombolo Yemodeli | i-cTnI/CK-MB/MYO | Ukupakisha | Izivivinyo ezingu-25/ikhithi, amakhithi angu-30/i-CTN |
| Igama | Ikhithi Yokuxilonga ye-Cardiac Troponin I ∕Isoenzyme MB ye-Creatine Kinase ∕Myoglobin | Ukuhlukaniswa kwezinsimbi | Isigaba II |
| Izici | Ukuzwela okuphezulu, Ukusebenza okulula | Isitifiketi | I-CE/ ISO13485 |
| Ukunemba | > 99% | Isikhathi sokuphelelwa yisikhathi | Iminyaka Emibili |
| Indlela Yokwenza | Ukuhlolwa kwe-Fluorescence Immunochromatographic | Isevisi ye-OEM/ODM | Kuyatholakala |
UKUSETSHENZISWA OKUHLOSIWE
Le khithi iyasebenza ekutholakaleni kwe-in vitro ngobuningi bokuhlushwa kwezimpawu zokulimala kwenhliziyo zenhliziyo
i-troponin I, i-isoenzyme MB ye-creatine kinasein kanye ne-myoglobin ku-serum/plasma/isampula yegazi lomuntu wonke, kanye
ifanele ukuxilongwa okusizayo kwe-myocardial infarction. Le khithi inikeza kuphela imiphumela yokuhlolwa kwe-cardiac troponin I,
i-isoenzyme MB ye-creatine kinasein kanye ne-myoglobin, kanye nemiphumela etholakele kufanele isetshenziswe kanye nezinye
Ulwazi lwezokwelapha ukuze luhlaziywe. Kumelwe lusetshenziswe ochwepheshe bezempilo kuphela.
Inqubo yokuhlola
| 1 | Ngaphambi kokusebenzisa i-reagent, funda ngokucophelela iphakheji bese ujwayelana nezinqubo zokusebenza. |
| 2 | Khetha imodi yokuhlola ejwayelekile ye-WIZ-A101 portable immune analyzer |
| 3 | Vula iphakethe lesikhwama se-aluminium foil eline-reagent bese ukhipha idivayisi yokuhlola. |
| 4 | Faka idivayisi yokuhlola ngokuvundlile endaweni yokuhlaziya amasosha omzimba. |
| 5 | Ekhasini lasekhaya le-interface yokusebenza ye-immune analyzer, chofoza okuthi “Standard” ukuze ufake i-interface yokuhlola. |
| 6 | Chofoza okuthi “QC Scan” ukuze uskene ikhodi ye-QR ngaphakathi kwekhithi; amapharamitha ahlobene nekhithi yokufaka kuthuluzi bese ukhetha uhlobo lwesampula. Qaphela: Inombolo ngayinye yebhetshi yekhithi kufanele iskeniwe kanye. Uma inombolo yebhetshi isiskeniwe, yeqa lesi sinyathelo. |
| 7 | Hlola ukuvumelana “kwegama lomkhiqizo”, “inombolo yeqoqo” njll. esibonakalayo sokuhlola ngolwazi oluselebula lekhithi. |
| 8 | Khipha isampula yokuxuba uma ulwazi luhambisana, engeza isampula yegazi engu-80μL/plasma/igazi lonke, bese uyixuba kahle; |
| 9 | Faka isixazululo esixutshwe kahle esingu-80µL esishiwo ngenhla emthonjeni wedivayisi yokuhlola; |
| 10 | Ngemva kokwengeza okuphelele kwesampula, chofoza okuthi “Isikhathi” bese isikhathi sokuhlola esisele sizoboniswa ngokuzenzakalelayo ku-interface. |
| 11 | I-immune analyzer izoqedela ngokuzenzakalelayo ukuhlolwa nokuhlaziywa lapho isikhathi sokuhlolwa sesifikile. |
| 12 | Ngemva kokuthi ukuhlolwa yi-immune analyzer sekuqediwe, umphumela wokuhlolwa uzoboniswa ku-interface yokuhlola noma ungabukwa "ngomlando" ekhasini lasekhaya le-interface yokusebenza. |
Qaphela: isampula ngayinye kufanele ifakwe ngepayipi elihlanzekile elilahlwayo ukuze kugwenywe ukungcola okuxubile.
Ukuphakama
Isikhathi sokuhlola: 10-15mins
Isitoreji: 2-30℃/36-86℉
Indlela Yokusebenza: Ukuhlolwa Kwe-Fluorescence Immunochromatographic
Isici:
• Ukuzwela okuphezulu
• ukufundwa kwemiphumela ngemizuzu eyi-15
• Ukusebenza okulula
• Ukuhlolwa oku-3 ngesikhathi esisodwa, okusindisa izikhathi.
• Ukunemba Okuphezulu
Ukusebenza Kwezokwelapha
Ukusebenza kahle kwalomkhiqizo kuhlolwe ngokuqoqwa kwamacala angu-150 amasampula omtholampilo.
a) Uma kwenzeka into ye-cTnI, ikhithi ehambisanayo yokuthengisa yokuhlola i-chemiluminescence isetshenziswa njenge-reference reagent,
imiphumela yokuthola iqhathanisiwe futhi ukuqhathanisa kwayo kufundwe ngokuhlelwa kabusha okuqondile, futhi
ama-coefficient okuhlobana kwalezi zivivinyo ezimbili yi-Y=0.975X+0.074 kanye ne-R=0.9854 ngokulandelana;
b) Uma kwenzeka into ye-CK-MB, ikhithi ehambisanayo yokuthengisa yokuhlolwa kwe-electrochemiluminescence isetshenziswa njengereferensi
i-reagent, imiphumela yokuthola iqhathanisiwe futhi ukuqhathanisa kwayo kufundwe ngokusebenzisa i-linear
ukuhlehla, kanye nama-coefficients okuhlobana kwalezi zivivinyo ezimbili yi-Y=0.915X+0.242 kanye ne-R=0.9885 ngokulandelana.
c) Uma kwenzeka into ye-MYO, ikhithi ethengiswayo ehambisanayo yokuhlolwa kwe-fluor immunoassays okuxazululwe ngesikhathi isetshenziswa njengereferensi
i-reagent, imiphumela yokuthola iqhathanisiwe futhi ukuqhathanisa kwayo kufundwe ngokusebenzisa i-linear
ukuhlehla, kanye nama-coefficients okuhlobana kwalezi zivivinyo ezimbili yi-y=0.989x+2.759 kanye ne-R=0.9897 ngokulandelana.
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