Pahu Hōʻailona no D-Dimer(hoʻāʻo immunochromatographic fluorescence)
No ka hoʻohana ʻana i ka diagnostic in vitro wale nō
E ʻoluʻolu e heluhelu pono i kēia mea hoʻokomo i loko o ka pūʻolo ma mua o ka hoʻohana ʻana a e hahai pono i nā kuhikuhi. ʻAʻole hiki ke hōʻoia ʻia ka hilinaʻi o nā hopena hoʻāʻo inā he mau ʻokoʻa mai nā kuhikuhi i loko o kēia mea hoʻokomo i loko o ka pūʻolo.

KA HOʻOHANA I MANAʻO ʻIA
ʻO ka pahu hōʻoia no ka D-Dimer (fluorescence immunochromatographic assay) he fluorescence immunochromatographic assay no ka ʻike ʻana i ka nui o D-Dimer (DD) i loko o ka plasma kanaka, hoʻohana ʻia no ka ʻike ʻana i ka venous thrombosis, disseminated intravascular coagulation, a me ka nānā ʻana i ka thrombolytic therapy. Pono e hōʻoia ʻia nā hāpana maikaʻi a pau e nā ʻano hana ʻē aʻe. Ua manaʻo ʻia kēia hoʻāʻo no ka hoʻohana ʻana o ka poʻe loea mālama ola kino wale nō.

HŌʻULUʻULU MANAʻO
Hōʻike ʻo DD i ka hana fibrinolytic. ʻO nā kumu no ka hoʻonui ʻia o DD: 1. ʻO ka hyperfibrinolysis lua, e like me ka hypercoagulation, disseminated intravascular coagulation, renal disease, organ transplant rejection, thrombolytic therapy, etc. 2. Aia nā hana thrombus i hoʻāla ʻia a me nā hana fibrinolysis i loko o nā kīʻaha koko; 3. Myocardial infarction, cerebral infarction, pulmonary embolism, venous thrombosis, ʻoki kino, tumor, diffuse intravascular coagulation, infection and tissue necrosis, etc.

KUMUHANA O KE KAʻINA HANA
Ua uhi ʻia ka membrane o ka mea hoʻāʻo me ka antibody anti DD ma ka ʻāpana hoʻāʻo a me ka antibody IgG kao anti rabbit ma ka ʻāpana kaohi. Ua uhi ʻia ka pad lepili e ka fluorescence i kapa ʻia ʻo anti DD antibody a me ka rabbit IgG ma mua. I ka hoʻāʻo ʻana i ka laʻana maikaʻi, hui pū ka antigen DD i loko o ka laʻana me ka fluorescence i kapa ʻia ʻo anti DD antibody, a hana i ka hui pale. Ma lalo o ka hana o ka immunochromatography, ke kahe nei ka paʻakikī i ke kuhikuhi o ka pepa absorbent, i ka wā i hala ai ka paʻakikī i ka ʻāpana hoʻāʻo, hui pū ʻia me ka antibody uhi anti DD, hana i kahi paʻakikī hou. Pili maikaʻi ka pae DD me ka hōʻailona fluorescence, a hiki ke ʻike ʻia ka nui o DD i loko o ka laʻana e ka fluorescence immunoassay assay.

Nā mea hoʻohui a me nā mea i hoʻolako ʻia

Nā ʻāpana pūʻolo 25T：
Kāleka hoʻāʻo pākahi i hoʻopaʻa ʻia me ka pepa alumini me kahi desiccant 25T
Nā mea hoʻoheheʻe laʻana 25T
Hoʻokomo pūʻolo 1

NĀ MEA I KOI ʻIA AKĀ ʻAʻOLE I HĀʻAWI ʻIA
Pahu hōʻiliʻili laʻana, uaki manawa

KA HŌʻILIʻILI A ME KA MĀLAMA ʻANA I NĀ HĀʻAWI LAʻANA
Hiki i nā hāpana i hoʻāʻo ʻia ke heparin anticoagulant plasma a i ʻole EDTA anticoagulant plasma.
Wahi a nā ʻano hana maʻamau, e hōʻiliʻili i ka laʻana. Hiki ke mālama ʻia ka laʻana serum a plasma paha i loko o ka friji ma 2-8 ℃ no 7 mau lā a me ka cryopreservation ma lalo o -15°C no 6 mau mahina.
Pale nā ​​hāpana āpau i nā pōʻaiapuni hau-hoʻoheheʻe.

KAʻINA HOʻĀʻO
E ʻoluʻolu e heluhelu i ka manual hana o ka mea hana a me ka mea hoʻokomo pūʻolo ma mua o ka hoʻāʻo ʻana.
1. E waiho i nā reagents a me nā laʻana a pau i ka mahana o ka lumi.
2. E wehe i ka Portable Immune Analyzer (WIZ-A101), e hoʻokomo i ka ʻōlelo huna moʻokāki e like me ke ʻano hana o ka mea hana, a e komo i ka ʻaoʻao ʻike.
3. E nānā i ke code dentification e hōʻoia i ka mea hoʻāʻo.
4. E lawe i waho i ke kāleka hoʻāʻo mai loko mai o ka ʻeke pepa kini.
5. E hoʻokomo i ke kāleka hoʻāʻo i loko o ke kau kāleka, e scan i ke code QR, a e hoʻoholo i ka mea hoʻāʻo.
6.E hoʻohui i ka 40μL serum a i ʻole ka plasma sample i ka diluent sample, a hui maikaʻi.
7. E hoʻohui i ka hopena laʻana 80μL i ka lua laʻana o ke kāleka.
8. Kaomi i ke pihi "hoʻāʻo maʻamau", ma hope o 15 mau minuke, e ʻike koke ka mea kani i ke kāleka hoʻāʻo, hiki iā ia ke heluhelu i nā hopena mai ka pale hōʻike o ka mea kani, a hoʻopaʻa/paʻi i nā hopena hoʻāʻo.
9. E nānā i nā kuhikuhi o ka Portable Immune Analyzer (WIZ-A101).

NĀ KUMUWAIWAI I MANAʻO ʻIA
DD <0.5mg/L
Manaʻo ʻia e hoʻokumu kēlā me kēia keʻena hoʻokolohua i kāna pae maʻamau ponoʻī e hōʻike ana i kāna heluna kanaka maʻi.

NĀ HUA HOʻĀʻO A ME KA WEHEWEHE ʻANA
ʻO ka ʻikepili ma luna nei ka hopena o ka hoʻāʻo reagent DD, a ua manaʻo ʻia e hoʻokumu kēlā me kēia keʻena hoʻokolohua i kahi laulā o nā waiwai ʻike DD kūpono no ka heluna kanaka ma kēia ʻāpana. No ka ʻike wale nō nā hopena ma luna.
Pili wale nā ​​hopena o kēia ʻano hana i nā pae kuhikuhi i hoʻokumu ʻia ma kēia ʻano hana, a ʻaʻohe hoʻohālikelike pololei me nā ʻano hana ʻē aʻe.
Hiki i nā kumu ʻē aʻe ke hana i nā hewa i nā hopena ʻike, me nā kumu loea, nā hewa hana a me nā kumu laʻana ʻē aʻe.

KA MĀLAMA ʻANA A ME KE KŪPAʻA
1. He 18 mau mahina ka lōʻihi o ka mālama ʻana o kēia pahu mai ka lā i hana ʻia ai. E mālama i nā pahu i hoʻohana ʻole ʻia ma 2-30°C. MAI HOʻOPAʻAHAU. Mai hoʻohana ma mua o ka lā palena pau.
2. Mai wehe i ka ʻeke i hoʻopaʻa ʻia a hiki i kou mākaukau e hana i kahi hoʻāʻo, a ua ʻōlelo ʻia e hoʻohana ʻia ka hoʻāʻo hoʻokahi hoʻohana ma lalo o ke kaiapuni e pono ai (mahana 2-35 ℃, haʻahaʻa 40-90%) i loko o 60 mau minuke me ka wikiwiki.
3. Hoʻohana koke ʻia ka mea hoʻoheheʻe laʻana ma hope o ka wehe ʻia ʻana.

NĀ ʻŌLELO AʻO A ME NĀ MEA E MĀLAMA AI
1. Pono e sila ʻia ka pahu a pale ʻia mai ka makū.
2. Pono e hōʻoia ʻia nā laʻana maikaʻi a pau e nā ʻano hana ʻē aʻe.
3. Pono e mālama ʻia nā hāpana a pau he mea haumia hiki ke loaʻa.
4. MAI hoʻohana i ka reagent i pau ka lā.
5. MAI hoʻololi i nā reagents ma waena o nā kits me nā helu lot like ʻole..
6. MAI hoʻohana hou i nā kāleka hoʻāʻo a me nā mea pono hoʻolei ʻia.
7.Misoperation, hāpana nui a liʻiliʻi paha ke alakaʻi i nā ʻokoʻa hopena.

LHOʻOHOLO
E like me nā hoʻāʻo āpau e hoʻohana ana i nā antibodies mouse, aia ka hiki ke hoʻopilikia ʻia e nā antibodies anti-mouse kanaka (HAMA) i loko o ka specimen. ʻO nā specimens mai nā maʻi i loaʻa nā hoʻomākaukau o nā monoclonal antibodies no ka hōʻoia ʻana a i ʻole ka lāʻau lapaʻau e loaʻa paha iā HAMA. Hiki i kēlā mau specimens ke hana i nā hopena maikaʻi ʻole a i ʻole nā ​​hopena maikaʻi ʻole.
ʻO kēia hopena hoʻāʻo no ka ʻike lapaʻau wale nō, ʻaʻole pono e lawelawe ma ke ʻano he kumu wale nō no ka ʻike lapaʻau a me ka mālama ʻana, pono e noʻonoʻo piha ʻia ka hoʻokele lapaʻau o nā maʻi me kāna mau hōʻailona, ​​​​ka mōʻaukala lapaʻau, nā hoʻokolohua keʻena ʻē aʻe, ka pane lapaʻau, ka epidemiology a me nā ʻike ʻē aʻe.
Hoʻohana wale ʻia kēia reagent no nā hoʻāʻo serum a me plasma. ʻAʻole paha e loaʻa ka hopena pololei ke hoʻohana ʻia no nā laʻana ʻē aʻe e like me ka saliva a me ka mimi a me nā mea ʻē aʻe.

NĀ ʻANO HANA

RNā Hōʻike
1.Hansen JH, et al.HAMA Ke komo ʻana me nā Immunoassays Murine Monoclonal Antibody-Based [J].J o Clin Immunoassay,1993,16:294-299.
2.Levinson SS. Ke ʻano o nā Antibodies Heterophilic a me ke kuleana i ka Immunoassay Interference[J].J o Clin Immunoassay,1992,15:108-114.

Nā kī i nā hōʻailona i hoʻohana ʻia:

	Mea Hana Lapaʻau Diagnostic In Vitro
	Mea Hana
	E mālama ma 2-30 ℃
	Ka lā pau
	Mai hoʻohana hou
	AKAHELE
	E nānā i nā kuhikuhi no ka hoʻohana ʻana

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