ʻO ka pahu Diagnostic no 25-hydroxy Vitamin D (fluorescence immunochromatographic assay)

wehewehe pōkole:


  • Ka manawa hoʻāʻo:10-15 minuke
  • Manawa kūpono:24 mahina
  • pololei:ʻOi aku ma mua o 99%
  • Hōʻike:1/25 hōʻike / pahu
  • Mahana mālama:2 ℃-30 ℃
  • Huahana Huahana

    Huahana Huahana

    ʻO ka pahu diagnostic no 25-hydroxy Vitamin D(ka ho'āʻo immunochromatographic fluorescence)
    No ka hoʻohana diagnostic in vitro wale nō

    E ʻoluʻolu e heluhelu pono i kēia pūʻolo hoʻokomo ma mua o ka hoʻohana ʻana a e hahai pono i nā ʻōlelo kuhikuhi.ʻAʻole hiki ke hōʻoiaʻiʻo ʻia ka hilinaʻi o nā hopena assay inā loaʻa kekahi mau ʻokoʻa mai nā ʻōlelo aʻo i loko o kēia pūʻolo hoʻokomo.

    KA MANAO ANA
    ʻO ka Diagnostic Kit no 25-hydroxy Vitamin D (fluorescence immunochromatographic assay) he fluorescence immunochromatographic assay no ka ʻike nui ʻana o 25-hydroxy Vitamin D (25-(OH)VD) i loko o ka serum kanaka a i ʻole ka plasma, i hoʻohana nui ʻia e loiloi i nā pae. ʻO ka huaʻa D. He mea kōkua hōʻailona hōʻailona.Hoʻohana ʻia kēia hoʻāʻo no ka ʻoihana mālama ola kino wale nō.

    HOOLAHA
    ʻO ka Vitamin D he huaʻaola a he hormone steroid pū kekahi, ʻo ia hoʻi me VD2 a me VD3, nona ke ʻano like.Hoʻololi ʻia ka Vitamin D3 a me D2 i 25 hydroxyl vitamin D (me 25-dihydroxyl vitamin D3 a me D2).25-(OH) VD i loko o ke kino kanaka, struction paʻa, kiʻekiʻe ka manaʻo.Hōʻike ka 25-(OH) VD i ka nui o ka huaora D, a me ka hiki ke hoʻololi i ka vitamina D, no laila ua manaʻo ʻia ʻo 25-(OH)VD ka hōʻailona maikaʻi loa no ka loiloi ʻana i ke kiʻekiʻe o ka vitamina D. Hoʻokumu ʻia ka Diagnostic Kit ma immunochromatography a hiki ke hāʻawi i kahi hopena i loko o 15 mau minuke.

    KUMU O KE HANA
    Hoʻopili ʻia ka membrane o ka mea hoʻāʻo me ka conjugate o BSA a me 25-(OH)VD ma ka ʻāpana hoʻāʻo a me ka anti rabbit IgG antibody ma ka ʻāpana hoʻomalu.Hoʻopili ʻia ka pahu marker e ka fluorescence mark anti 25-(OH)VD antibody a me ka rabbit IgG ma mua.I ka hoʻāʻo ʻana i ka laʻana, 25-(OH)VD i loko o ka laʻana e hui pū me ka fluorescence i kaha ʻia i ka anti 25-(OH)VD antibody, a hana i ka hui ʻole.Ma lalo o ka hana o ka immunochromatography, ka paʻakikī kahe i ke kuhikuhi o ka absorbent pepa, i ka wā paʻakikī i hala i ka ho'āʻo 'āina, E hui 'ia ka mea hoailona fluorescent manuahi me 25-(OH)VD ma ka membrane.The concentration of 25-(OH) ʻO VD ka pilina maikaʻi ʻole no ka hōʻailona fluorescence, a hiki ke ʻike ʻia ke ʻano o 25-(OH)VD i ka laʻana e ka fluorescence immunoassay assay.

    NA REAGENA a me na lako i hoolakoia

    25T pūʻolo mea
    .Kale ho'āʻo pakahi pahu pepa me ka desiccant 25T
    .He haʻina 25T
    .B hāʻina 1
    .Hoʻokomo pūʻolo 1

    MAKEMAKE IA NA MEA AKA AOLE I HAAWI MAI
    Laʻana hōʻiliʻili ipu, manawa

    KA OHI A ME KA WAIWAI
    1. ʻO nā mea hoʻohālike i ho'āʻoʻia he serum, heparin anticoagulant plasma a iʻole EDTA anticoagulant plasma.

    2.According i nā hana maʻamau e hōʻiliʻili i ka hāpana.Hiki ke mālama ʻia ka serum a i ʻole plasma sample i loko o ka friji ma 2-8 ℃ no 7 mau lā a me ka cryopreservation ma lalo o -15 ° C no 6 mau mahina.
    3.All laʻana e pale i nā pōʻaiapuni freeze-thaw.

    HANA HOIKE
    ʻO ke kaʻina hana hoʻāʻo o ka mea kani e ʻike i ka manual immunoanalyzer.Penei ke kaʻina hana ho'āʻo reagent

    1. E waiho i nā reagents a me nā laʻana i ka lumi wela.
    2. E wehe i ka Portable Immune Analyzer (WIZ-A101), e hoʻokomo i ka ʻōlelo huna huna e like me ke ʻano hana o ka mea kani, a komo i ka ʻike ʻike.
    3. E nānā i ke code dentification e hōʻoia i ka mea hoʻāʻo.
    4. E lawe i ke kāleka hōʻoia mai ka ʻeke pepa.
    5. E hoʻokomo i ke kāleka hōʻike i loko o ka pahu kāleka, e nānā i ka QR code, a hoʻoholo i ka mea hōʻike.
    6.Add 30μL serum a plasma sample i A solution, a hui pono.
    7.Add 50μL B solution i ka hui luna, a hui maikaʻi.
    8.E waiho i ka hui no 15 mau minuke.
    9.Add 80μL hui 'ia e laʻana maikaʻi o ke kāleka.
    10. Kaomi i ke pihi "ho'āʻo maʻamau", ma hope o 10 mau minuke, e ʻike koke ka mea kani i ka kāleka hōʻike, hiki iā ia ke heluhelu i nā hopena mai ka pale hōʻike o ka mea kani, a hoʻopaʻa / paʻi i nā hopena hoʻāʻo.
    11. E nānā i ke aʻo ʻana o Portable Immune Analyzer (WIZ-A101).

    NA WAIWAI I MANAOIA
    25-(OH)VD laulā maʻamau:30-100ng/mL

    Manaʻo ʻia e hoʻokumu kēlā me kēia keʻena hana i kona ʻano maʻamau e hōʻike ana i kona heluna maʻi.

    HUA HOʻOʻO A ME KA HOʻOKAʻANA
    .ʻO ka ʻikepili ma luna nei ka wā kuhikuhi i hoʻokumu ʻia no ka ʻike ʻike ʻana o kēia pahu, a ua manaʻo ʻia e hoʻokumu kēlā me kēia keʻena keʻena i kahi manawa kuhikuhi no ka nui o ke olakino pili o ka heluna kanaka ma kēia māhele.

    .ʻOi aku ka kiʻekiʻe o ka manaʻo o 25-(OH)VD ma mua o ka pae kuhikuhi, a pono e kāpae ʻia nā hoʻololi physiological a i ʻole ke koʻikoʻi.
    .Pili wale nā ​​hopena o kēia ʻano i ka pae kuhikuhi i hoʻokumu ʻia e kēia ʻano, a ʻaʻole i hoʻohālikelike pono nā hopena me nā ʻano hana ʻē aʻe.
    . Hiki i nā kumu ʻē aʻe ke hana hewa i nā hopena ʻike, me nā kumu ʻenehana, nā hewa hana a me nā kumu hoʻohālike ʻē aʻe.

    HOOLAHA A ME KA PALAPALA
    .He 18 mau mahina o ke ola o ka pahu mai ka lā i hana ʻia ai.E mālama i nā pahu i hoʻohana ʻole ʻia ma 2-30 ° C.MAI HOOMAIKAI.Mai hoʻohana ma mua o ka lā pau.

    .Mai wehe i ka pahu i hoʻopaʻa ʻia a hiki i kou mākaukau e hana i kahi hoʻāʻo, a manaʻo ʻia ka hoʻohana hoʻohana hoʻokahi e hoʻohana ʻia ma lalo o ke kaiapuni i koi ʻia (ka mahana 2-35 ℃, haʻahaʻa 40-90%) i loko o 60 mins e like me ka wikiwiki. hiki.
    .Hoʻohana koke ʻia ka diluent laʻana ma hope o ka wehe ʻana.

    OLELO HOOLAHA A ME KA MALAMA
    . Pono e hoʻopaʻa ʻia ka pahu a pale ʻia mai ka makū.

    .E hōʻoia ʻia nā hōʻailona maikaʻi a pau e nā ʻano hana ʻē aʻe.
    .E mālama ʻia nā mea hoʻohālike a pau ma ke ʻano he mea haumia.
    Mai hoʻohana i ka reagent i pau.
    Mai hoʻololi i nā reagents ma waena o nā pahu me nā ʻokoʻa ʻole.
    Mai hoʻohana hou i nā kāleka hoʻāʻo a me nā mea pono hoʻopau.
    .Misoperation, excessive or liʻiliʻi hāpana hiki ke alakai i ka hopena deviations.

    LHOOLAHA
    .E like me ka hoʻohana ʻana i nā antibodies ʻiole, hiki ke hoʻopilikia ʻia e ke kanaka anti-mouse antibodies (HAMA) i ka specimen.Hiki ke loaʻa ka HAMA i nā hōʻailona mai nā maʻi i loaʻa ka hoʻomākaukau ʻana o nā antibodies monoclonal no ka maʻi a i ʻole ka lāʻau lapaʻau.Hiki i ia mau hiʻohiʻona ke hoʻopuka i nā hopena maikaʻi ʻole a i ʻole nā ​​hopena maikaʻi ʻole.

    ʻO kēia hopena hoʻokolohua wale nō no ka hōʻike lapaʻau, ʻaʻole pono e lilo i kumu hoʻokahi no ka maʻi maʻi a me ka mālama ʻana, pono e noʻonoʻo ʻia nā mea maʻi i ka mālama ʻana i ka maʻi me kāna mau hōʻailona, ​​ka mōʻaukala olakino, nā hoʻokolohua ʻē aʻe, ka pane lapaʻau, epidemiology a me nā ʻike ʻē aʻe. .
    .Hoʻohana wale ʻia kēia reagent no nā hoʻokolohua serum a me ka plasma.ʻAʻole paha e loaʻa ka hopena pololei ke hoʻohana ʻia no nā laʻana ʻē aʻe e like me ka saliva a me ka mimi a me nā mea ʻē aʻe.

    NA HIO HANA

    Linearity 5 ng/mL i ka 120 ng/mL kaʻawale pili:-15% a +15%.
    Huina huikau laina:(r)≥0.9900
    Ka pololei Aia ka helu ho'ōla ma loko o 85% - 115%.
    Hiki hou CV≤15%
    kikoo
    (ʻAʻohe o nā mea i hoʻāʻo ʻia i hoʻopili ʻia i ka hoʻāʻo)
    Hoʻopilikia Hoʻopili hoʻopaʻapaʻa
    Hemoglobin 200μg/mL
    transferrin 100μg/mL
    ʻO ka radish peroxidase 2000μg/mL
    Huaola D3 50mg/mL
    Huaola D 50mg/mL

    RHOOLAHA

    1.Hansen JH,et al.HAMA Interference with Murine Monoclonal Antibody-Based Immunoassays[J].J of Clin Immunoassay,1993,16:294-299.
    2.Levinson SS.Ke 'ano o Heterophilic Antibodies a me ke kuleana i ka Immunoassay Interference[J].J of Clin Immunoassay,1992,15:108-114.

    Ki i nā hōʻailona i hoʻohana ʻia:

     t11-1 In Vitro Diagnostic Lapaʻau Lapaʻau
     tt-2 Mea hana
     tt-71 E mālama i ka 2-30 ℃
     tt-3 Ka lā pau
     tt-4 Mai hoohana hou
     tt-5 ALOHA
     tt-6 E kūkākūkā i nā kuhikuhi no ka hoʻohana ʻana

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