Diagnostic Kit ya 25-hydroxy Vitamini D(fluorescence immunochromatographic assay)
Kwa in vitro diagnostic ntchito kokha
Chonde werengani phukusili Ikani mosamala musanagwiritse ntchito ndikutsatira malangizowo.Kudalirika kwa zotsatira zoyeserera sikungatsimikizidwe ngati pali zolakwika zilizonse kuchokera pamalangizo omwe ali mu phukusili.

ZOYENERA KUGWIRITSA NTCHITO
Diagnostic Kit ya 25-hydroxy Vitamini D (fluorescence immunochromatographic assay) ndi fluorescence immunochromatographic assay pakuzindikira kuchuluka kwa 25-hydroxy Vitamin D (25-(OH) VD) mu seramu yamunthu kapena plasma, yomwe imagwiritsidwa ntchito makamaka kuyesa milingo. wa vitamini D. Ndi chithandizo chothandizira matenda. Zitsanzo zonse zabwino ziyenera kutsimikiziridwa ndi njira zina.Kuyezetsaku kumangogwiritsidwa ntchito ndi akatswiri azachipatala okha.

CHIDULE
Vitamini D ndi vitamini komanso ndi mahomoni a steroid, makamaka VD2 ndi VD3, omwe malangizo awo ndi ofanana kwambiri.Vitamini D3 ndi D2 amasinthidwa kukhala 25 hydroxyl vitamini D (kuphatikiza 25-dihydroxyl vitamini D3 ndi D2).25-(OH) VD m'thupi la munthu, malangizo okhazikika, okwera kwambiri.25-(OH) VD imasonyeza kuchuluka kwa vitamini D, ndi mphamvu ya kutembenuka kwa vitamini D, kotero 25-(OH) VD imatengedwa kuti ndi chizindikiro chabwino kwambiri chowunika mlingo wa vitamini D. The Diagnostic Kit imachokera immunochromatography ndipo amatha kupereka zotsatira mkati mwa mphindi 15.

MFUNDO YA NJIRA
Nembanemba ya chipangizo choyesera imakutidwa ndi conjugate ya BSA ndi 25-(OH) VD pagawo loyeserera ndi anti-anti rabbit IgG antibody pagawo lowongolera.Marker pad amakutidwa ndi fluorescence mark anti 25-(OH) VD antibody ndi kalulu IgG pasadakhale.Mukayesa zitsanzo, 25-(OH) VD mu zitsanzo phatikizani ndi fluorescence zolembedwa anti 25-(OH)VD antibody, ndi kupanga osakaniza chitetezo.Pansi pa zochita za immunochromatography, kuyenda movutikira kunjira ya pepala loyamwa, pamene zovuta zidadutsa gawo loyesa, Chizindikiro cha fulorosenti yaulere chidzaphatikizidwa ndi 25-(OH) VD pa nembanemba.Kuphatikizika kwa 25-(OH) VD ndikulumikizana koyipa kwa siginecha ya fluorescence, ndipo kuchuluka kwa 25-(OH) VD mu zitsanzo kumatha kuzindikirika ndi fluorescence immunoassay assay.

REAGENTS NDI Zipangizo ZOPEREKA

Zigawo za phukusi la 25T:
.Khadi loyesera payokha zojambulazo zopakidwa ndi desiccant 25T
.A yankho 25T
.B yankho 1
.Phukusi loyika 1

ZINTHU ZOFUNIKA KOMA ZOSAPATSIDWA
Zitsanzo zosonkhanitsira chidebe, timer

KUSONKHA ZITSANZO NDI KUSINTHA
1.Zitsanzo zoyesedwa zingakhale seramu, heparin anticoagulant plasma kapena EDTA anticoagulant plasma.
2.Malinga ndi njira zoyenera sonkhanitsani zitsanzo.Seramu kapena madzi a m'magazi amatha kusungidwa mufiriji pa 2-8 ℃ kwa 7days ndi cryopreservation pansi -15 ° C kwa miyezi 6.
3.Zitsanzo zonse zimapewa kuzizira kozizira.

NTCHITO YOSANGALALA
Njira yoyesera ya chida onani buku la immunoanalyzer.Njira yoyeserera ya reagent ili motere
1.Ikani pambali ma reagents onse ndi zitsanzo kutentha kutentha.
2.Tsegulani Portable Immune Analyzer (WIZ-A101), lowetsani mawu achinsinsi a akaunti malinga ndi njira yogwiritsira ntchito chida, ndikulowetsani mawonekedwe ozindikira.
3.Scan code dentification kuti mutsimikizire chinthu choyesera.
4.Tulutsani khadi loyesera muthumba la zojambulazo.
5.Lowetsani khadi loyesera mu kagawo ka khadi, jambulani kachidindo ka QR, ndi kuzindikira chinthu choyesera.
6.Onjezani 30μL seramu kapena chitsanzo cha plasma ku A yankho, ndikusakaniza bwino.
7.Onjezani 50μL B yankho ku osakaniza pamwamba, ndi kusakaniza bwino.
8.Siyani osakaniza kwa mphindi 15.
9.Onjezani 80μL osakaniza kuti muyese bwino khadi.
10.Dinani batani la "standard test", pakatha mphindi 10, chidacho chimangozindikira khadi yoyeserera, imatha kuwerenga zotsatira kuchokera pachiwonetsero cha chidacho, ndikulemba / kusindikiza zotsatira zoyeserera.
11. Onani malangizo a Portable Immune Analyzer(WIZ-A101).

MFUNDO ZOYENERA
25-(OH)VD yachibadwa osiyanasiyana: 30-100ng/mL
Ndikoyenera kuti labotale iliyonse ikhazikitse mitundu yakeyake yomwe ikuyimira odwala ake.

ZOTSATIRA ZA MAYESE NDI KUMASULIRA
.Deta yomwe ili pamwambayi ndi nthawi yolozera yomwe yakhazikitsidwa kuti ipeze deta ya zidazi, ndipo akulangizidwa kuti labotale iliyonse iyenera kukhazikitsa nthawi yowonetsera kufunikira kwachipatala kwa anthu m'dera lino.
.Chiwerengero cha 25-(OH) VD ndi chapamwamba kusiyana ndi chiwerengero chofotokozera, ndipo kusintha kwa thupi kapena kupsinjika maganizo kuyenera kuchotsedwa.
.Zotsatira za njirayi zimangogwiritsidwa ntchito pazidziwitso zomwe zakhazikitsidwa ndi njira iyi, ndipo zotsatira zake sizingafanane mwachindunji ndi njira zina.
.Zinthu zina zingayambitsenso zolakwika pazotsatira zodziwika, kuphatikizapo zifukwa zamakono, zolakwika zogwirira ntchito ndi zitsanzo zina.

KUSINTHA NDI KUKHALA
.Zidazi ndi miyezi 18 ya alumali kuyambira tsiku lopangidwa.Sungani zida zosagwiritsidwa ntchito pa 2-30 ° C.OSATI MASIMA.Musagwiritse ntchito kupitirira tsiku lotha ntchito.
.Musatsegule thumba losindikizidwa mpaka mutakonzeka kuyesa, ndipo kuyesa kamodzi kokha kumalangizidwa kuti agwiritsidwe ntchito pansi pa malo ofunikira (kutentha kwa 2-35 ℃, chinyezi 40-90%) mkati mwa mphindi 60 mwamsanga zotheka.
.Sample diluent amagwiritsidwa ntchito atangotsegulidwa.

CHENJEZO NDI CHENJEZO
.Chidacho chiyenera kutsekedwa ndi kutetezedwa ku chinyezi.
.Zitsanzo zonse zabwino zidzatsimikiziridwa ndi njira zina.
.Zitsanzo zonse ziyenera kuonedwa ngati zowononga.
.MUSAMAGWIRITSE NTCHITO zomwe zatha.
.OSATI kusinthanitsa ma reagents pakati pa zida zomwe zili ndi manambala osiyanasiyana.
.MUSAMAGWIRITSE NTCHITO makhadi oyesera ndi zina zilizonse zomwe zingathe kutayidwa.
.Misoperation, mochulukira kapena pang'ono zitsanzo kungayambitse zotsatira zopotoka.

LKUTSANZITSA
.Mofanana ndi kuyesa kulikonse komwe kumagwiritsa ntchito ma antibodies a mbewa, kuthekera kulipo kuti kusokonezedwe ndi ma antibodies a anti-mbewa (HAMA) pachitsanzo.Zitsanzo za odwala omwe alandira kukonzekera kwa ma antibodies a monoclonal kuti adziwe matenda kapena mankhwala angakhale ndi HAMA.Zitsanzo zoterezi zingayambitse zotsatira zabodza kapena zabodza.
.Chotsatira choyezetsa ichi ndi chachipatala chokha, sichiyenera kukhala maziko okhawo a matenda ndi chithandizo chamankhwala, chithandizo chamankhwala cha odwala chiyenera kuganiziridwa mozama pamodzi ndi zizindikiro zake, mbiri yachipatala, kufufuza kwa labotale, kuyankha kwamankhwala, miliri ndi zina. .
.Reagent iyi imagwiritsidwa ntchito poyezetsa seramu ndi plasma.Ikhoza kusapeza zotsatira zolondola zikagwiritsidwa ntchito pa zitsanzo zina monga malovu ndi mkodzo ndi zina.

ZINTHU ZOCHITIKA

RMALANGIZO

1.Hansen JH, ndi al.HAMA Kusokoneza ndi Murine Monoclonal Antibody-Based Immunoassays [J].J ya Clin Immunoassay,1993,16:294-299.
2.Levinson SS.Chilengedwe cha Heterophilic Antibodies ndi Udindo mu Immunoassay Interference[J].J wa Clin Immunoassay,1992,15:108-114.

Chinsinsi cha zizindikiro zogwiritsidwa ntchito:

	In Vitro Diagnostic Medical Chipangizo
	Wopanga
	Sungani pa 2-30 ℃
	Tsiku lothera ntchito
	Osagwiritsanso Ntchito
	CHENJEZO
	Funsani Malangizo Ogwiritsa Ntchito

Xiamen Wiz Biotech CO., LTD
Address:3-4 Floor,NO.16 Building,Bio-medical Workshop,2030 Wengjiao West Road,Haicang District,361026,Xiamen,China
Tel: + 86-592-6808278
Fax: + 86-592-6808279