Kete Tohu mo te 25-hydroxy Huaora D(whakamatautau immunochromatographic fluorescence)
Mo te whakamahi tātaritanga in vitro anake
Tena koa panuihia tenei kete whakauru i mua i te whakamahi me te whai i nga tohutohu.Kare e taea te whakapumau i te pono o nga hua whakamatautau mena he rereke nga tohutohu kei roto i tenei kete whakauru.

WHAKAMAHI WHAKAMAHI
Ko te Kete Tohu mo te 25-hydroxy Huaora D (fluorescence immunochromatographic assay) he whakamatautau immunochromatographic fluorescence mo te kitenga ine o te 25-hydroxy Huaora D (25-(OH)VD) i roto i te serum tangata, plasma ranei, e whakamahia ana hei arotake i nga taumata. o te huaora D.He reagent tātaritanga awhina.Me whakau nga tauira pai katoa e etahi atu tikanga.Ko tenei whakamatautau he tikanga mo te whakamahi ngaio hauora anake.

KAUPAPA
Ko te Huaora D he huaora, he homoni steroid ano hoki, ko te nuinga ko te VD2 me te VD3, he tino rite te hanganga.Ka hurihia te Huaora D3 me te D2 ki te 25 hydroxyl vitamin D (tae atu ki te 25-dihydroxyl vitamin D3 me D2).25-(OH) VD i roto i te tinana tangata, struction pūmau, kukū tiketike.Ko te 25-(OH) VD e whakaatu ana i te tapeke o te huaora D , me te kaha o te hurihanga o te huaora D, na ko te 25-(OH)VD ka kiia ko te tohu pai rawa atu mo te arotake i te taumata o te huaora D. Ko te Kete Tohu Whakaata kei runga immunochromatography a ka puta he hua i roto i te 15 meneti.

MĀTĀPONO O TE TIKANGA
Ko te kiriuhi o te taputapu whakamatautau ka pania ki te conjugate o te BSA me te 25-(OH)VD i te rohe whakamatautau me te koati anti rabbit IgG antibody i runga i te rohe whakahaere.Ka pania te papa tohu ki te tohu fluorescence anti 25-(OH)VD antibody me te IgG rapeti i mua.I te wa e whakamatautau ana i te tauira, 25-(OH)VD i roto i te tauira ka whakakotahi me te rewharewha kua tohua he anti 25-(OH)VD antibody, ka hanga i te ranunga aukati.I raro i te mahi a te immunochromatography, ka rere te matatini ki te huarahi o te pepa absorbent, i te wa i pahemo te matatini i te rohe whakamatautau, Ko te tohu rererangi kore utu ka hono ki te 25- (OH) VD i runga i te membrane.Ko te kukū o te 25- (OH) Ko te VD he hononga toraro mo te tohu rewharewha, a ka kitea te kukū o te 25-(OH)VD i roto i te tauira ma te whakamatautau immunoassay fluorescence.

NGA REAGENTS ME NGA MAUTANGA I TUKUA

25T nga waahanga kete：
.Kari whakamatautau takitahi he konumohe kua mau ki te whakamaroke 25T
.He otinga 25T
.B otinga 1
.Pake whakauru 1

NGĀ KAUPAPA KAUPAPA ENGARI KAORE I TE WHAKAMAHI
ipu kohinga tauira, taima

KOHIHI TAUIRA ME TE WHAKAPUTANGA
1.Ko nga tauira whakamatautau ka taea te serum, heparin anticoagulant plasma ranei EDTA anticoagulant plasma.
2.E ai ki nga tikanga paerewa kohikohi tauira.Ka taea te pupuri i te serum, te tauira plasma ranei i roto i te pouaka whakamahana i te 2-8 ℃ mo te 7 ra me te cryopreservation i raro -15°C mo te 6 marama.
3.Katoa nga tauira ka karo i nga huringa whakatio-rewa.

TE WHAKAMAHI WHAKAMAHI
Ko te tikanga whakamatautau o te taputapu tirohia te pukapuka immunoanalyzer.Ko te tukanga whakamatautau reagent e whai ake nei
1. Waiho nga reagents me nga tauira katoa ki te taha mahana.
2.Whakatuwheratia te Portable Immune Analyzer(WIZ-A101), tomo i te takiuru kupuhipa pūkete kia rite ki te tikanga mahi o te taputapu, ka uru ki te atanga kitenga.
3.Matawaia te tohu tohu hei whakau i te mea whakamatautau.
4.Tangohia te kaari whakamatautau mai i te putea pepa.
5. Whakauruhia te kaari whakamatautau ki roto i te mokamoka kaari, matawai i te waehere QR, ka whakatau i te mea whakamatautau.
6. Tāpirihia te 30μL serum, te tauira plasma ranei ki te otinga A, ka uru pai.
7. Tāpirihia he otinga 50μL B ki te ranunga i runga ake nei, ka uru pai.
8.Waiho te ranunga mo te 15 meneti.
9. Tāpirihia te ranunga 80μL hei tauira pai o te kaari.
10. Patohia te paatene "whakamatautau paerewa", i muri i nga meneti 10, ka kitea e te taputapu te kaari whakamatautau, ka taea e ia te panui i nga hua mai i te mata whakaatu o te taputapu, me te tuhi / tuhi i nga hua whakamatautau.
11. Tirohia te tohutohu a Portable Immune Analyzer(WIZ-A101).

NGĀ UARA E TUKUA
25-(OH)VD awhe noa:30-100ng/mL
E taunaki ana kia whakatuu ia taiwhanga i tana ake awhe noa hei tohu mo te taupori turoro.

WHAKAMAHI ME TE WHAKAMAHI
.Ko nga raraunga i runga ake nei ko te waahi tohutoro kua whakaritea mo nga raraunga rapunga o tenei kete, me te whakaaro me whakatu e ia taiwhanga he waahi tohutoro mo te hiranga haumanu e tika ana mo te taupori o tenei rohe.
.Ko te kukū o te 25-(OH)VD he teitei ake i te awhe tohutoro, a me whakakorehia nga huringa whaiaroaro, te whakautu ahotea ranei.Ko te mea he rereke, me whakakotahi i nga tohu tohu haumanu.
.Ko nga hua o tenei tikanga e pa ana ki te awhe tohutoro kua whakaritea e tenei tikanga, a, ko nga hua kaore e tino rite ki etahi atu tikanga.
.Ka taea hoki e etahi atu take te hapa i nga hua kitenga, tae atu ki nga take hangarau, nga hapa whakahaere me etahi atu tauira tauira.

TE WHAKAPUTANGA ME TE WHAKANUI
.Ko te kete he 18 marama te roa o te oranga mai i te ra i hangaia ai.Whakaorangia nga kete kaore i whakamahia ki te 2-30°C.KAUA E WHAKAMATE.Kaua e whakamahi i tua atu i te ra paunga.
.Kaua e whakatuwheratia te putea hiri kia rite ra ano koe ki te mahi i tetahi whakamatautau, ka whakaarohia te whakamatautau kotahi-whakamahi kia whakamahia i raro i te taiao e hiahiatia ana (te mahana 2-35 ℃, te haumākū 40-90%) i roto i te 60 mins i te tere. taea.
.Ka whakamahia te diluent tauira i muri tonu i te whakatuwheratanga.

WHAKATOKANGA ME TE WHAKATAKI
.Me hiri te kete me te tiaki i te makuku.
.Ko nga tauira pai katoa ka whakamanahia e etahi atu tikanga.
.Ko nga tauira katoa ka kiia he poke pea.
KAUA e whakamahi reagent kua pau.
KAUA e whakawhitia nga reagents i waenga i nga kete he rereke te rota kore..
.KAUA e whakamahi ano i nga kaari whakamatautau me nga taputapu tuku.
.Ko te mahi pohehe, te nui, te iti ranei o te tauira ka puta he rerekee nga hua.

LWHAKATAHI
.Ka rite ki nga whakamatautau e whakamahi ana i nga paturopi kiore, tera pea ka pokanoa e nga paturopi anti-kiore tangata (HAMA) i roto i te tauira.Ko nga tauira mai i nga turoro kua whiwhi i nga whakaritenga o nga paturopi monoclonal mo te taatai ​​​​me te rongoa kei roto pea te HAMA.Ko enei tauira ka puta he hua pai, he hua kino ranei.
.Ko tenei hua whakamatautau mo te tohutoro haumanu anake, kaua e waiho hei kaupapa anake mo te taatai ​​haumanu me te maimoatanga, ko te whakahaere haumanu o nga turoro me whai whakaaro matawhānui me ona tohu, hitori hitori, etahi atu whakamatautau taiwhanga, whakautu maimoatanga, mate urutomo me etahi atu korero .
.Ka whakamahia noa tenei reagent mo nga whakamatautau serum me te plasma.Kaore pea e puta he hua tika ina whakamahia mo etahi atu tauira penei i te huware me te mimi me etahi atu.

TE WHAKAMAHI

RWHAKAMAHI

1.Hansen JH,et al.HAMA Interference with Murine Monoclonal Antibody-Based Immunoassays[J].J of Clin Immunoassay,1993,16:294-299.
2.Levinson SS.Ko te Nature of Heterophilic Antibodies and the Role in Immunoassay Interference[J].J of Clin Immunoassay,1992,15:108-114.

Te matua mo nga tohu e whakamahia ana:

	In Vitro Diagnostic Medical Device
	Kaihanga
	Rokiroki ki te 2-30 ℃
	Te Ra Whakamutunga
	Kaua e Whakamahi Anō
	WHAKATAHI
	Tirohia nga Tohutohu mo te Whakamahi

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