Kete Tātari mo te Cardiac Troponin I (whakamātautau immunochromatographic fluorescence)
Kete Tohu mo te Cardiac Troponin I(whakamatautau immunochromatographic fluorescence)
Mo te whakamahi tātaritanga in vitro anake
Tena koa panuihia tenei kete whakauru i mua i te whakamahi me te whai i nga tohutohu.Kare e taea te whakapumau i te pono o nga hua whakamatautau mena he rereke nga tohutohu kei roto i tenei kete whakauru.
WHAKAMAHI WHAKAMAHI
Ko te Kete Tohu mo te Cardiac Troponin I (fluorescence immunochromatographic assay) he whakamatautau immunochromatographic fluorescence mo te kitenga ine o te Cardiac Troponin I (cTnI) i roto i te serum tangata, plasma ranei, ka whakamahia mo te tātaritanga awhina o AMI (Acute Myocardial Infarction).Ko nga tauira pai katoa me whakamana e etahi atu tikanga.Ko tenei whakamatautau he tikanga mo te whakamahi ngaio hauora anake.
KAUPAPA
Ka piki ake nga taumata cTnI i etahi haora i muri mai i te paheketanga o te Myocardial infarction, ka eke ki runga i te 12-16 haora, ka piki ake i te 4-9 ra i muri i te paheketanga o te Myocardial infarction.Ko te whakamaramatanga o te ao mo te toru o nga mate o te ngakau i te tau 2012: Ko te tohu koiora-cTn(I, T ranei) e pai ana, he tino motuhake te kiko o te myocardial me te aro nui ki te haumanu.Ko nga huringa i roto i te kukū o te cTn he mea nui ki te tātaritanga o te AMI
MĀTĀPONO O TE TIKANGA
Ko te kiriuhi o te taputapu whakamatautau ka pania ki te anti cTnI antibody i runga i te rohe whakamatautau me te koati anti rabbit IgG antibody i runga i te rohe whakahaere.Ka whakakikoruatia te papa tapanga ki te rewharewha kua tapaina he anti cTnI antibody me te IgG rapeti i mua.I te wa e whakamatautau ana i te tauira pai, ka hono te antigen cTnI i roto i te tauira me te fluorescence kua tapaina he antibody anti cTnI, ka hanga he ranunga aukati.I raro i te mahi o te immunochromatography, te rere matatini i roto i te ahunga o te pepa absorbent, ka pahemo matatini te rohe whakamātautau, ngā reira ki anti cTnI paninga antibody, hanga hou complex.cTnI taumata he pai taurite ki tohu fluorescence, me te kukū o cTnI i roto i te tauira ka kitea e te fluorescence immunoassay assay.
NGA REAGENTS ME NGA MAUTANGA I TUKUA
25T nga waahanga kete：
Kari whakamatautau takitahi he konumohe kua mau ki te whakamaroke 25T
Tauira waimeha 25T
Kōkuhu mōkihi 1
NGĀ KAUPAPA KAUPAPA ENGARI KAORE I TE WHAKAMAHI
ipu kohinga tauira, taima
KOHIHI TAUIRA ME TE WHAKAPUTANGA
1.Ko nga tauira whakamatautau ka taea te serum, heparin anticoagulant plasma ranei EDTA anticoagulant plasma.
2.E ai ki nga tikanga paerewa kohikohi tauira.Ka taea te pupuri i te serum, te tauira plasma ranei i roto i te pouaka whakamahana i te 2-8 ℃ mo te 7 ra me te cryopreservation i raro -15°C mo te 6 marama.
3.Katoa nga tauira ka karo i nga huringa whakatio-rewa.
TE WHAKAMAHI WHAKAMAHI
Tena koa panuihia te pukapuka mahi taputapu me te whakauru mokihi i mua i te whakamatautau.
1. Waiho nga reagents me nga tauira katoa ki te taha mahana.
2.Whakatuwheratia te Portable Immune Analyzer(WIZ-A101), tomo i te takiuru kupuhipa pūkete kia rite ki te tikanga mahi o te taputapu, ka uru ki te atanga kitenga.
3.Matawaia te tohu tohu hei whakau i te mea whakamatautau.
4.Tangohia te kaari whakamatautau mai i te putea pepa.
5. Whakauruhia te kaari whakamatautau ki roto i te mokamoka kaari, matawai i te waehere QR, ka whakatau i te mea whakamatautau.
6. Tāpirihia he 40μL serum, he tauira plasma ranei hei tauira i te waimeha, ka uru pai.
7. Tāpirihia he otinga tauira 80μL hei tauira pai o te kaari.
8.Patohia te paatene "whakamatautau paerewa", i muri i nga meneti 15, ka kitea e te taputapu te kaari whakamatautau, ka taea e ia te panui i nga hua mai i te mata whakaatu o te taputapu, me te tuhi / tuhi i nga hua whakamatautau.
9. Tirohia te tohutohu a Portable Immune Analyzer(WIZ-A101).
NGĀ UARA E TUKUA
cTnI <0.3ng/mL
E taunaki ana kia whakatuu ia taiwhanga i tana ake awhe noa hei tohu mo te taupori turoro.
WHAKAMAHI ME TE WHAKAMAHI
.Ko nga raraunga o runga ake nei ko te hua o te whakamatautau reagent cTnI, a ko te whakaaro me whakatu e ia taiwhanga he awhe o nga uara rapu cTnI e tika ana mo te taupori o tenei rohe.Ko nga hua i runga ake nei hei tohutoro anake.
.Ko nga hua o tenei tikanga e pa ana ki nga awhe tohutoro kua whakaritea i roto i tenei tikanga, a kaore he whakataurite tika ki etahi atu tikanga.
.Ka taea hoki e etahi atu take te hapa i nga hua kitenga, tae atu ki nga take hangarau, nga hapa whakahaere me etahi atu tauira tauira.
TE WHAKAPUTANGA ME TE WHAKANUI
1.Ko te kete he 18 marama te roa o te oranga mai i te ra i hangaia ai.Whakaorangia nga kete kaore i whakamahia ki te 2-30°C.KAUA E WHAKAMATE.Kaua e whakamahi i tua atu i te ra paunga.
2.Kaua e whakatuwheratia te putea hiri kia rite ra ano koe ki te mahi i tetahi whakamatautau, a ko te whakamatautau kotahi-whakamahi ka whakaarohia kia whakamahia i raro i te taiao e hiahiatia ana (te mahana 2-35 ℃, te haumākū 40-90%) i roto i te 60 mins tere. ka taea.
3.Ka whakamahia te diluent tauira i muri tonu i te whakatuwheratanga.
WHAKATOKANGA ME TE WHAKATAKI
.Me hiri te kete me te tiaki i te makuku.
.Ko nga tauira pai katoa ka whakamanahia e etahi atu tikanga.
.Ko nga tauira katoa ka kiia he poke pea.
KAUA e whakamahi reagent kua pau.
KAUA e whakawhitia nga reagents i waenga i nga kete me nga rota rereke No.
.KAUA e whakamahi ano i nga kaari whakamatautau me nga taputapu tuku.
.Ko te mahi pohehe, te nui, te iti ranei o te tauira ka puta he rerekee nga hua.
LWHAKATAHI
.Ka rite ki nga whakamatautau e whakamahi ana i nga paturopi kiore, tera pea ka pokanoa e nga paturopi anti-kiore tangata (HAMA) i roto i te tauira.Ko nga tauira mai i nga turoro kua whiwhi i nga whakaritenga o nga paturopi monoclonal mo te taatai me te rongoa kei roto pea te HAMA.Ko enei tauira ka puta he hua pai, he hua kino ranei.
.Ko tenei hua whakamatautau mo te tohutoro haumanu anake, kaua e waiho hei kaupapa anake mo te taatai haumanu me te maimoatanga, ko te whakahaere haumanu o nga turoro me whai whakaaro matawhānui me ona tohu, hitori hitori, etahi atu whakamatautau taiwhanga, whakautu maimoatanga, mate urutomo me etahi atu korero .
.Ka whakamahia noa tenei reagent mo nga whakamatautau serum me te plasma.Kaore pea e puta he hua tika ina whakamahia mo etahi atu tauira penei i te huware me te mimi me etahi atu.
TE WHAKAMAHI
| Rarangirangi | 0.1ng/mL ki te 40ng/mL | inenga whanaunga:-15% ki te +15%. |
| Ko te whakarea hononga raina:(r)≥0.9900 | ||
| Tika | Ko te tere whakaora i roto i te 85% - 115%. | |
| Te tukurua | CV≤15% | |
| Te motuhake(Kaore tetahi o nga matū o te pokanoa i whakamatauria i pokanoa ki te whakamatautau) | Whakararuraru | Te kukū pokanoa |
| sTnI | 1000μg/L | |
| cTnT | 1000μg/L | |
| ABP | 1000μg/L | |
| CK-MB | 1000μg/L | |
| cTnC | 1000μg/L | |
| sTnT | 1000μg/L | |
| TAKU | 1000μg/L | |
RWHAKAMAHI
1.Hansen JH,et al.HAMA Interference with Murine Monoclonal Antibody-Based Immunoassays[J].J of Clin Immunoassay,1993,16:294-299.
2.Levinson SS.Ko te Nature of Heterophilic Antibodies and the Role in Immunoassay Interference[J].J of Clin Immunoassay,1992,15:108-114.
Te matua mo nga tohu e whakamahia ana:
 | In Vitro Diagnostic Medical Device |
 | Kaihanga |
 | Rokiroki ki te 2-30 ℃ |
 | Te Ra Whakamutunga |
 | Kaua e Whakamahi Anō |
 | WHAKATAHI |
 | Tirohia nga Tohutohu mo te Whakamahi |
Xiamen Wiz Biotech CO.,LTD
Wāhi noho: 3-4 Papa, NO.16 Whare, Bio-medical Workshop, 2030 Wengjiao West Road, Haicang District, 361026, Xiamen, Haina
Waea:+86-592-6808278
Waeatuhi:+86-592-6808279
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