Igikoresho cyo gusuzuma indwara ya Cardiac Troponin I.(fluorescence immunochromatographic assay)
Kuri vitro kwisuzumisha koresha gusa
Nyamuneka soma iyi paki shyiramo witonze mbere yo gukoresha kandi ukurikize neza amabwiriza.Ibisubizo byizewe ntibishobora kwemezwa niba hari gutandukana kwamabwiriza muriyi paki yinjizamo.

UKORESHEJWE
Igikoresho cyo gusuzuma indwara ya Cardiac Troponin I (fluorescence immunochromatographic assay) ni fluorescence immunochromatographic assay yo kumenya umubare wa Cardiac Troponin I (cTnI) muri serumu yumuntu cyangwa plasma, ikoreshwa mugupima ubufasha bwa AMI (Acute Myocardial Infarction).Icyitegererezo cyiza cyose kigomba kwemezwa nubundi buryo.Iki kizamini kigenewe ubuvuzi bwumwuga gusa.

INCAMAKE
Urwego rwa CTnI rwiyongereye nyuma yamasaha menshi nyuma yindwara ya Myocardial ibaye, igera kumasaha 12-16, kandi ikomeza kuzamuka nyuma yiminsi 4-9 nyuma yindwara ya Myocardial.Ubusobanuro bwisi yose ya infocarite ya gatatu ya myocardial mumwaka wa 2012: Biyomarker-cTn (I cyangwa T) ikunzwe, ifite myocardial tissue yihariye kandi ifite ivuriro ryinshi.Impinduka murwego rwa cTn ningirakamaro mugupima AMI

IHame RY'UBURYO
Membrane yigikoresho cyipimisha yashizwemo antibody ya anti cTnI mukarere ka test hamwe na antibody yihene irwanya urukwavu IgG mukarere kayobora.Lable pad yashizwemo na fluorescence yanditseho antibody ya cTnI ninkwavu IgG mbere.Iyo ugerageza icyitegererezo cyiza, antigen ya cTnI murugero ihuza na fluorescence yanditseho antibody ya anti cTnI, hanyuma igakora imvange yumubiri.Mubikorwa bya immunochromatografiya, urujya n'uruza rugana mu cyerekezo cyimpapuro zinjira, iyo complexe yanyuze mukarere kizamini, yahujwe na anti-cTnI coating antibody, ikora urwego rushya.cTnI urwego rufitanye isano neza nibimenyetso bya fluorescence, hamwe nubunini bwa cTnI murugero rushobora gutahurwa na fluorescence immunoassay assay.

REAGENTS N'IBIKORWA BYUZUWE

25T ibice：
Ikarita yikizamini kugiti cyayo ifatanye na desiccant 25T
Icyitegererezo cya 25T
Shyiramo paki 1

IBIKORWA BISABWA ARIKO NTIBITANZWE
Icyitegererezo cyo gukusanya ibikoresho, igihe

GUKORANYA URUGERO N'UBubiko
1.Ingero zapimwe zishobora kuba serumu, heparin anticoagulant plasma cyangwa plasma ya EDTA anticoagulant.
2.Kurikije tekiniki zisanzwe zegeranya icyitegererezo.Icyitegererezo cya serumu cyangwa plasma birashobora kubikwa muri firigo kuri 2-8 ℃ muminsi 7 hamwe no kubika munsi -15 ° C mumezi 6.
3.Icyitegererezo cyose wirinde gukonjesha.

GUKORA UBURYO
Nyamuneka soma igitabo gikoreshwa nigikoresho cyo gushyiramo mbere yo kugerageza.
1. Shira ku ruhande reagent zose hamwe nicyitegererezo kubushyuhe bwicyumba.
2.Fungura Portable Immune Analyser (WIZ-A101), andika ijambo ryibanga rya konte ukurikije uburyo bukoreshwa bwigikoresho, hanyuma winjire muburyo bwo kumenya.
3.Shobora kode ya dentification kugirango wemeze ikintu cyizamini.
4.Kuramo ikarita yikizamini mu mufuka wa file.
5. Shyiramo ikarita yikizamini ahantu h'ikarita, suzuma kode ya QR, hanyuma umenye ikintu cyizamini.
6. Ongeramo 40μL serumu cyangwa plasma icyitegererezo kugirango utange urugero, hanyuma uvange neza ..
7. Ongeraho 80μL igisubizo cyicyitegererezo kugirango utange neza ikarita.
8.Kanda buto ya "ikizamini gisanzwe", nyuma yiminota 15, igikoresho kizahita kimenya ikarita yikizamini, irashobora gusoma ibisubizo bivuye kuri ecran yerekana igikoresho, hanyuma wandike / wandike ibisubizo byikizamini.
9. Reba ku mabwiriza ya Portable Immune Analyser (WIZ-A101).

AGACIRO KATEGANYIJWE
cTnI <0.3ng / mL
Birasabwa ko buri laboratoire ishyiraho urwego rwayo rusanzwe ruhagarariye abaturage barwaye.

IBISUBIZO BY'IKIZAMINI NO GUSOBANURA
.Amakuru yavuzwe haruguru ni ibisubizo byikizamini cya cTnI, kandi hasabwa ko buri laboratoire igomba gushyiraho indangagaciro za cTnI zerekana neza abaturage muri kano karere.Ibisubizo byavuzwe haruguru nibisobanuro gusa.
.Ibisubizo byubu buryo birakoreshwa gusa murwego rwoherejwe rwashyizweho muri ubu buryo, kandi ntaho bihuriye nubundi buryo.
.Ibindi bintu birashobora kandi gutera amakosa mubisubizo byo gutahura, harimo impamvu za tekiniki, amakosa yimikorere nibindi bintu byintangarugero.

Ububiko N'UBUHAMYA
1.Ibikoresho ni amezi 18 yo kubaho-uhereye igihe byakorewe.Bika ibikoresho bidakoreshwa kuri 2-30 ° C.NTUBUNTU.Ntukoreshe kurenza itariki izarangiriraho.
2.Ntukingure umufuka ufunze kugeza igihe witeguye gukora ikizamini, kandi ikizamini kimwe cyo gukoreshwa gisabwa gukoreshwa mubisabwa (ubushyuhe 2-35 ℃, ubuhehere 40-90%) muminota 60 byihuse. bishoboka.
3.Urugero rwa diluent rukoreshwa ako kanya nyuma yo gufungura.

UMUBURO N'UBWITONDERWA
.Ibikoresho bigomba gufungwa kandi bikarindwa ubushuhe.
.Ingero zose nziza zemezwa nubundi buryo.
.Ingero zose zifatwa nkibishobora guhumanya.
.Ntugakoreshe reagent yarangiye.
.NtUGASIMBURE reagent mubikoresho bifite ubufindo butandukanye Oya ..
.NtUGASUBIZE gukoresha amakarita yikizamini nibikoresho byose bikoreshwa.
.Gukoresha nabi, urugero rwinshi cyangwa ruto rushobora kuganisha ku gutandukana.

LKwigana
.Nkuko hamwe nubushakashatsi ubwo aribwo bwose bukoresha antibodi yimbeba, birashoboka ko habaho kwivanga na antibodiyite zabantu barwanya imbeba (HAMA) murugero.Ingero z'abarwayi bahawe imyiteguro ya antibodiyite ya monoclonal yo gusuzuma cyangwa kuvura irashobora kuba irimo HAMA.Ingero nkizo zishobora gutera ibinyoma byiza cyangwa ibinyoma bibi.
.Ibisubizo by'ibizamini ni ibyerekeye ivuriro gusa, ntibigomba kuba ishingiro ryonyine ryo gusuzuma no kuvura kwa muganga, imicungire y’amavuriro y’abarwayi igomba kwitabwaho byimazeyo hamwe n’ibimenyetso byayo, amateka y’ubuvuzi, ibindi bizamini bya laboratoire, igisubizo cy’ubuvuzi, epidemiologiya nandi makuru .
.Iyi reagent ikoreshwa gusa mugupima serumu na plasma.Ntishobora kubona ibisubizo nyabyo mugihe ikoreshejwe izindi ngero nka macandwe ninkari nibindi.

IBIKORWA BIKORWA

RINGARUKA

1.Hansen JH, n'abandi.HAMA Kwivanga kwa Murine Monoclonal Antibody ishingiye kuri Immunoassays [J] .J ya Clin Immunoassay, 1993,16: 294-299.
2.Levinson S.

Urufunguzo rwibimenyetso byakoreshejwe:

	Mubikoresho byubuvuzi bya Vitro
	Uruganda
	Ubike kuri 2-30 ℃
	Itariki izarangiriraho
	Ntugakoreshe
	ICYITONDERWA
	Baza Amabwiriza yo Gukoresha

Xiamen Wiz Biotech CO., LTD
Aderesi: Igorofa 3-4, Inyubako.16
Tel: + 86-592-6808278
Fax: + 86-592-6808279