Ngwa Nchọpụta maka D-Dimer(nnwale immunochromatographic fluorescence)
Maka ojiji nchọpụta n'ime vitro naanị
Biko gụọ ihe e tinyere na ngwugwu a nke ọma tupu e jiri ya, ma gbasoo ntuziaka ndị a nke ọma. A gaghị ekwe nkwa na nsonaazụ nnwale ga-adabere na ntụkwasị obi ma ọ bụrụ na e nwere ihe ọ bụla na-adịghị mma na ntuziaka dị na ngwugwu a.

Ebumnobi Ojiji
Ngwa Nchọpụta maka D-Dimer (nnwale fluorescence immunochromatographic) bụ nnwale fluorescence immunochromatographic maka nchọpụta ọnụọgụ nke D-Dimer (DD) na plasma mmadụ. A na-eji ya maka nchọpụta nke thrombosis venous, mgbasa nke coagulation intravascular, na nlekota nke ọgwụgwọ thrombolytic. A ga-eji usoro ndị ọzọ kwado ihe nlele niile dị mma. Emebere ule a maka ojiji ndị ọkachamara ahụike naanị.

Nchịkọta
DD na-egosipụta ọrụ fibrinolytic. Ihe kpatara mmụba nke DD: 1. Hyperfibrinolysis nke abụọ, dịka hypercoagulation, mgbasa nke coagulation intravascular, ọrịa akụrụ, ịjụ transplant akụkụ ahụ, ọgwụgwọ thrombolytic, wdg. 2. Enwere ọrụ nhazi thrombus na fibrinolysis na arịa; 3. Myocardial infarction, cerebral infarction, pulmonary embolism, venous thrombosis, ịwa ahụ, tumor, diffuse intravascular coagulation, infection and tissue necrosis, wdg.

ỤKPỤRỤ NKE ỤZỌ A
A na-ekpuchi akpụkpọ ahụ nke ngwaọrụ nnwale ahụ na mgbochi DD antibody na mpaghara nnwale ahụ na mgbochi IgG antibody nke ewu na mpaghara njikwa. A na-ekpuchi paadị lable site na iji fluorescence nke a na-akpọ anti DD antibody na oke bekee IgG tupu oge eruo. Mgbe a na-anwale ihe nlele dị mma, antigen DD dị na ihe nlele ahụ na-ejikọta ya na antibody anti DD nke a na-akpọ fluorescence, ma na-emepụta ngwakọta mgbochi. N'okpuru ọrụ nke immunochromatography, usoro mgbagwoju anya na-aga n'ihu nke akwụkwọ na-amịkọrọ ihe, mgbe mgbagwoju anya gafere mpaghara nnwale ahụ, ọ jikọtara ya na antibody mkpuchi anti DD, na-emepụta mgbagwoju anya ọhụrụ. Ọkwa DD na-ejikọta nke ọma na mgbaàmà fluorescence, a pụkwara ịchọpụta ntinye nke DD na ihe nlele ahụ site na nnwale immunoassay fluorescence.

Ihe ndị na-eme ka ihe dị nro na ihe ndị e ji mee ihe dị iche iche

Ihe mejupụtara ngwugwu 25T:
Kaadị nnwale n'otu n'otu nke ejiri foil kpuchie akpa ya na ihe nhicha mmiri 25T
Ihe ndị na-agbaze ihe nlele 25T
Ntinye ngwugwu 1

A CHỌRỌ IHE E JI MEE YA MA E NWEGHỊ YA
Ihe nlele nchịkọta akpa, ihe ngụ oge

Nchịkọta na Nchekwa ihe nlele
Ihe nlele ndị a nwalere nwere ike ịbụ heparin anticoagulant plasma ma ọ bụ EDTA anticoagulant plasma.
Dịka usoro ọkọlọtọ si dị, a pụrụ iji ihe nlele serum ma ọ bụ plasma debe ya na friji na okpomọkụ 2-8℃ ruo ụbọchị asaa, ebe a na-echekwa ihe nlele n'okpuru -15°C ruo ọnwa isii.
.Ihe nlele niile na-ezere usoro ịgbaze na ịgbaze.

Usoro Nnwale
Biko gụọ akwụkwọ ntuziaka ọrụ ngwa ahụ na ihe e tinyere na ngwugwu tupu ị nwaa ya.
1. Debe ihe niile e ji eme ihe na ihe nlele n'akụkụ okpomọkụ ụlọ.
2. Mepee Portable Immune Analyzer (WIZ-A101), tinye paswọọdụ akaụntụ dịka usoro ọrụ nke ngwa ahụ si dị, wee tinye interface nchọpụta.
3. Lelee koodu dentification iji kwado ihe nnwale ahụ.
4. Wepụ kaadị ule ahụ n'ime akpa foil ahụ.
5. Tinye kaadị ule ahụ n'ime oghere kaadị ahụ, lelee koodu QR ahụ, wee chọpụta ihe nnwale ahụ.
6. Tinye ihe nlele serum ma ọ bụ plasma 40μL na ihe nlele mmiri, wee gwakọta ya nke ọma.
7. Tinye ihe ngwọta ihe nlele 80μL iji nwalee nke ọma nke kaadị ahụ.
8. Pịa bọtịnụ "ule ọkọlọtọ", mgbe nkeji iri na ise gachara, ngwaọrụ ahụ ga-achọpụta kaadị nnwale ahụ na akpaghị aka, ọ nwere ike ịgụ nsonaazụ sitere na ihuenyo ngosipụta nke ngwaọrụ ahụ, ma dekọọ/bipụta nsonaazụ nnwale ahụ.
9. Lee ntuziaka nke Portable Immune Analyzer (WIZ-A101).

Uru A Na-atụ Anya Ya
DD <0.5mg/L
A na-atụ aro ka ụlọ nyocha ọ bụla guzobe oke nke ya nke na-anọchite anya ọnụọgụ ndị ọrịa ya.

Nsonaazụ na Nkọwa nke Ule
.Ihe ndị a dị n'elu bụ nsonaazụ nke ule DD reagent, a na-atụkwa aro na ụlọ nyocha ọ bụla kwesịrị ịmepụta ọtụtụ uru nchọpụta DD dabara adaba maka ndị bi na mpaghara a. Nsonaazụ ndị a dị n'elu bụ maka ntụaka naanị.
. Nsonaazụ nke usoro a metụtara naanị oke ntụaka e guzobere n'ụzọ a, ọ dịghịkwa ntụnyere kpọmkwem na ụzọ ndị ọzọ.
.Ihe ndị ọzọ nwekwara ike ịkpata mmejọ na nsonaazụ nchọpụta, gụnyere ihe ndị metụtara teknụzụ, mmejọ arụmọrụ na ihe atụ ndị ọzọ.

Nchekwa na nkwụsi ike
1. Ngwa a ga-adịru ọnwa iri na asatọ site na ụbọchị e mepụtara ya. Debe ngwa ndị a na-ejighị na okpomọkụ 2-30°C. EKPERELA FRỊA. Ejila ya karịa ụbọchị njedebe.
2. Emeghela akpa ahụ e mechiri emechi ruo mgbe ị dị njikere ime nnwale, a na-atụkwa aro ka e jiri ule ahụ otu ugboro mee ihe n'okpuru ebe achọrọ (okpomọkụ 2-35℃, iru mmiri 40-90%) n'ime nkeji iri isii ozugbo enwere ike.
3. A na-eji ihe nlele sample ozugbo emepechara ya.

ỊDỌ AKA NA NTỊ NA ỊKPACHICHE
1. A ga-emechi ngwa ahụ ma chebe ya pụọ ​​na mmiri.
2. A ga-eji usoro ndị ọzọ kwado ihe nlele niile dị mma.
3. A ga-emeso ihe nlele niile dị ka ihe nwere ike imetọ ihe.
4.EJILA ihe na-eme ka ihe ghara ịdị irè.
5.EJILA mgbanwe ihe ndị na-emegharị ihe n'etiti ngwa ndị ahụ na ọtụtụ dị iche iche.
6. EJILA kaadị ule na ihe ndị ọzọ a na-atụfu eme ihe ọzọ.
7.Ọgwụrụ na-ezighi ezi, oke ma ọ bụ obere ihe nlele nwere ike ibute nsonaazụ na-adịghị mma.

LNṄỤRỤ
Dịka ọ dị na nnwale ọ bụla ejiri ọgwụ mgbochi òké mee, o nwere ike inwe nsogbu site na ọgwụ mgbochi òké mmadụ (HAMA) na ihe nlele ahụ. Ihe nlele sitere n'aka ndị ọrịa natara ọgwụ mgbochi monoclonal maka nchọpụta ma ọ bụ ọgwụgwọ nwere ike ịnwe HAMA. Ihe nlele ndị dị otú ahụ nwere ike ibute nsonaazụ ụgha ma ọ bụ nsonaazụ na-adịghị mma.
.Nsonaazụ ule a bụ naanị maka ntụaka ahụike, ekwesịghị ịbụ naanị ntọala maka nchọpụta na ọgwụgwọ ahụike, njikwa ahụike nke ndị ọrịa kwesịrị ịtụle nke ọma yana mgbaàmà ya, akụkọ ahụike, nyocha ụlọ nyocha ndị ọzọ, nzaghachi ọgwụgwọ, ọrịa na-efe efe na ozi ndị ọzọ.
A na-eji ihe a eme ihe naanị maka ule serum na plasma. O nwere ike ọ gaghị enweta nsonaazụ ziri ezi mgbe ejiri ya mee ihe nlele ndị ọzọ dịka ọnụ mmiri na mmamịrị na ihe ndị ọzọ.

ÀGWÀ ÀGWÀ Arụmọrụ

RNtụaka
1. Hansen JH, na ndị ọzọ. Mgbochi HAMA na Mbelata Mgbochi Mgbukpọ nke Dabere na Mgbukpọ Monoclonal [J].J nke Clin Immunoassay, 1993,16:294-299.
2. Levinson SS. Ọdịdị nke Ọgwụ Mgbochi Heterophilic na Ọrụ dị na Mgbochi Immunoassay[J].J nke Clin Immunoassay, 1992,15:108-114.

Isi ihe nnọchianya ejiri mee ihe:

	Ngwaọrụ Ahụike Nchọpụta In Vitro
	Onye nrụpụta
	Chekwaa na okpomọkụ 2-30℃
	Ụbọchị mmebi
	Ejila ya ọzọ
	ỊDỌ AKA NA NTỊ
	Lelee Ntuziaka Maka Ojiji

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