Agakoresho ko gusuzuma indwara ka D-Dimer(isuzuma rya fluorescence immunochromatographic assay)
Gukoreshwa mu gusuzuma indwara mu buryo bwa "in vitro" gusa
Mbere yo gukoresha iyi paki, soma witonze kandi ukurikize amabwiriza neza. Ntabwo bishoboka kwemeza ko ibisubizo by'isuzuma ari ukuri niba hari ibitagenda neza ku mabwiriza ari muri iyi paki.

IKORESHWA RYITEGANYANYE
Ikizamini cyo gusuzuma D-Dimer (ikizamini cya fluorescence immunochromatographic assay) ni ikizamini cya fluorescence immunochromatographic assay cyo gupima D-Dimer (DD) mu maraso y'umuntu, gikoreshwa mu gusuzuma thrombosis yo mu mitsi, gufungana kw'imitsi mu mitsi, no gukurikirana uburyo bwo kuvura thrombolytic. Ikizamini cyose cyagaragaye kigomba kwemezwa n'ubundi buryo. Iki kizamini kigenewe gukoreshwa n'abaganga gusa.

INSHAMAKE
DD igaragaza imikorere ya fibrinolytic. Impamvu zituma DD yiyongera: 1. Hyperfibrinolysis ya kabiri, nko kugabanya amaraso mu mitsi, kugabanya amaraso mu mitsi, indwara z'impyiko, kwanga ko ingingo zishyirwa mu yindi, kuvura indwara zo mu mitsi, nibindi. 2. Hariho ibikorwa byo kurema thrombus no kugabanya amaraso mu mitsi; 3. Kugabanya amaraso mu mitsi, kugabanya amaraso mu bwonko, kugabanya amaraso mu bihaha, kugabanya amaraso mu mitsi, kubagwa, ikibyimba, kugabanya amaraso mu mitsi, kwandura no kugabanya amaraso mu mitsi, nibindi.

IHAME RY'UBURYO BWAYO
Agace k'igikoresho cyo gupima gatwikiriwe na anti-DD antibody ku gace k'igeragezwa naho anti-IgG antibody yo mu ihene ku gace k'igenzura. Udupapuro two gupima dutwikiriwe na anti-DD antibody twanditseho fluorescence na IgG y'urukwavu mbere y'igihe. Mu gusuzuma icyitegererezo cyagaragaje ko ari cyiza, anti-DD antibody iri mu cyitegererezo ihuzwa na anti-DD antibody yanditseho fluorescence, maze igakora uruvange rw'ubudahangarwa bw'umubiri. Mu gihe cy'imikorere ya immunochromatography, urujya n'uruza rw'uruvange mu cyerekezo cy'impapuro zifata, iyo complex inyuze mu gace k'igeragezwa, ihujwe na anti-DD coating antibody, ikora complex nshya. Urwego rwa DD rufitanye isano ryiza n'ikimenyetso cya fluorescence, kandi ubwinshi bwa DD mu cyitegererezo bushobora kumenyekana hakoreshejwe isuzuma rya fluorescence immunoassay.

IBIKORESHO N'IBIKORESHO BYATANZWE

Ibice by'ipaki bya 25T:
Ikarita yo gupimisha ifite agapfunyika k'umuti wo koza amenyo ka 25T
Ingero z'ibinyabutabire 25T
Ipaki ikubiyemo 1

IBIKORESHO BIRAKENEWE ARIKO NTIBYATANGWA
Agasanduku ko gukusanya ingero, igihe

ICYITEGEREREZO CYO GUKORESHA NO KUBIKA
Ingero zapimwe zishobora kuba plasma ya heparin anticoagulant cyangwa plasma ya EDTA anticoagulant.
Dukurikije uburyo busanzwe bwo gukusanya icyitegererezo. Icyitegererezo cya serum cyangwa plasma gishobora kubikwa muri firigo kuri 2-8°C mu gihe cy'iminsi 7 no kubikwa munsi ya -15°C mu gihe cy'amezi 6.
Ingero zose zirinda gukonjesha no gushonga.

UBURYO BWO GUKORA IKIGANIRO
Mbere yo gupima, soma igitabo cy'amabwiriza agenga imikorere y'igikoresho n'agasanduku k'ibikoresho.
1. Shyira ku ruhande ibikoresho byose bikoreshwa mu gupima no mu gupima ingero ku bushyuhe bw'icyumba.
2. Fungura Portable Immune Analyzer (WIZ-A101), andika ijambo ry'ibanga ryinjira muri konti ukurikije uburyo bw'imikorere y'igikoresho, hanyuma winjize aho ureba.
3.Skena kode y'isuzuma kugira ngo wemeze ikintu cy'ikizamini.
4. Kura ikarita yo gupima mu gafuka k'ifishi.
5. Shyiramo ikarita y'ikizamini mu mwanya w'ikarita, skana kode ya QR, hanyuma umenye ikintu cy'ikizamini.
6. Ongeramo 40μL ya serum cyangwa plasma mu gipimo cy'icyitegererezo, hanyuma uvange neza.
7. Ongeraho umuti w'icyitegererezo wa 80μL ku iriba ry'ikarita.
8. Kanda kuri buto "ikizamini gisanzwe", nyuma y'iminota 15, igikoresho kizahita kibona ikarita y'ikizamini, gishobora gusoma ibisubizo bivuye kuri ecran y'igikoresho, no kwandika/gucapa ibisubizo by'ikizamini.
9. Reba amabwiriza ya Portable Immune Analyzer (WIZ-A101).

INDANGAGACIRO ZITEGANYIJWE
DD <0.5mg/L
Birasabwa ko buri laboratwari ishyiraho urwego rwayo rusanzwe rugaragaza umubare w’abarwayi bayo.

IBISUBIZO BY'IBIGERAGEZO N'UBUSOBANURO
.Amakuru yavuzwe haruguru ni ibyavuye mu isuzuma rya DD reagent, kandi birasabwa ko buri laboratwari igomba gushyiraho ubwoko butandukanye bw'agaciro ko gupima DD gakwiriye abaturage bo muri aka karere. Ibisubizo byavuzwe haruguru ni ibyo kwifashisha gusa.
Ibisubizo by'ubu buryo bireba gusa ingero z'ingero zashyizweho muri ubu buryo, kandi nta kugereranya kutaziguye n'ubundi buryo.
Izindi mpamvu zishobora gutera amakosa mu bisubizo byo kuvumbura, harimo impamvu za tekiniki, amakosa mu mikorere n'izindi mpamvu z'icyitegererezo.

UBUBIKO N'UBUTABE
1. Iyi mashini ishobora kumara amezi 18 ikoreshwa kuva ikozwe. Bika ibikoresho bitakoreshejwe kuri dogere selisiyusi 2-30. NTUBIKORESHE. Ntukabikoreshe nyuma y'itariki ntarengwa.
2. Ntugafungure agafuka gafunze kugeza igihe uzaba witeguye gukora ikizamini, kandi ikizamini gikoreshwa rimwe gusa ni byiza ko gikoreshwa mu gihe gikenewe (ubushyuhe 2-35℃, ubushuhe 40-90%) mu minota 60 vuba bishoboka.
3. Icyitegererezo cy'umuti uvangwa n'amazi gikoreshwa ako kanya nyuma yo gufungurwa.

IMIBURO N'IBYITONDERWA
1. Agakoresho kagomba kuba gafunze kandi karinzwe ubushuhe.
2. Ingero zose z’ibyavuye mu bushakashatsi zigomba kwemezwa n’ubundi buryo.
3. Ingero zose z'ibipimo bigomba gufatwa nk'ibintu bishobora kwanduza.
4.NTUKORESHE reagent yarengeje igihe.
5. NTUGAHINDURE ibyuma bitanga ubuziranenge hagati y'ibikoresho bifite umubare utandukanye w'ibikoresho.
6. NTUGAKORESHE amakarita yo gupima n'ibindi bikoresho byose bishobora gukoreshwa.
7. Gukoresha nabi, urugero rurenze urugero cyangwa ruto bishobora gutuma ibisubizo bihinduka.

LKWIGANA
Nkuko bigenda ku isuzuma ryose rikoresha uturemangingo tw’imbeba, birashoboka ko uturemangingo tw’imbeba (HAMA) twangiritse mu gipimo. Ingero z’abarwayi bahawe imiti y’uturemangingo tw’imbeba kugira ngo bapimwe cyangwa bavurwe zishobora kuba zirimo HAMA. Ingero nk’izo zishobora gutera ibisubizo bitari byo by’ukuri cyangwa bitari ukuri.
Ibi bisubizo ni ibyo kwitabwaho mu buvuzi gusa, ntabwo bigomba kuba ishingiro ryonyine ryo gusuzuma no kuvura abarwayi, uburyo bwo kuvura abarwayi bugomba kwitabwaho mu buryo bwuzuye hamwe n'ibimenyetso byabo, amateka y'ubuzima bwabo, ubundi buryo bwo gusuzuma muri laboratwari, uko ubuvuzi bwaba buhagaze, ibyorezo by'indwara n'andi makuru.
Iyi reagent ikoreshwa gusa mu gupima amaraso mu maraso no mu maraso. Ishobora kutabona igisubizo nyacyo iyo ikoreshejwe mu bindi bipimo nk'amacandwe n'inkari n'ibindi.

IBIRANGA IMIKORERE

RIBIREBANA
1.Hansen JH, et al.HAMA Interference hamwe n'ibizamini by'imyuka bishingiye ku murine monoclonal antibody [J].J bya Clin Immunoassay, 1993,16:294-299.
2.Levinson SS. Imiterere y'uturemangingo tw'umubiri duto duto n'uruhare mu gutera ikibazo cy'ubudahangarwa bw'umubiri [J].J ya Clin Immunoassay, 1992,15:108-114.

Urufunguzo rw'ibimenyetso byakoreshejwe:

	Igikoresho cyo kwa muganga gisuzuma indwara muri Vitro
	Uruganda
	Bika ahantu hari ubushyuhe buri hagati ya 2 na 30°C
	Itariki izarangiriraho
	Ntukongere gukoresha
	UBWITONDE
	Reba amabwiriza yo gukoresha

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