KUMUHANA

Ua uhi ʻia ka membrane o ka mea hoʻāʻo me ka antigen Rotavirus Group A ma ka ʻāpana hoʻāʻo a me ka antibody IgG kao anti rabbit ma ka ʻāpana kaohi. Ua uhi ʻia ka pad lepili e ka fluorescence i kapa ʻia ʻo anti Rotavirus Group A a me rabbit IgG ma mua. I ka hoʻāʻo ʻana i ka laʻana maikaʻi, hui pū ka RV i loko o ka laʻana me ka fluorescence i kapa ʻia ʻo anti Rotavirus Group A, a hana i ka hui pale. Ma lalo o ka hana o ka immunochromatography, ke kahe nei ka paʻakikī i ke kuhikuhi o ka pepa absorbent. Ke hala ka paʻakikī i ka ʻāpana hoʻāʻo, hui pū ʻia me ka antibody uhi anti-Rotavirus Group A, hana i kahi paʻakikī hou. Inā maikaʻi ʻole, ʻaʻohe antigen Rotavirus Group A i loko o ka laʻana, no laila ʻaʻole hiki ke hoʻokumu ʻia nā paʻakikī pale, ʻaʻohe laina ʻulaʻula ma ka wahi ʻike (T). Me ka nānā ʻole inā aia ka rotavirus hui A i loko o ka laʻana, ua chromatograph ʻia ka latex-labeled mouse IgG i ka wahi kaohi maikaʻi (C) a hopu ʻia e ka antibody IgG kao anti-mouse. E ʻike ʻia kahi laina ʻulaʻula ma ka wahi kaohi maikaʻi (C). ʻO ka laina ʻulaʻula ke kūlana maʻamau e kū mai ana ma ka ʻāpana kaohi maikaʻi (C) no ka hoʻoholo ʻana inā lawa nā laʻana a inā he maʻamau ke kaʻina hana chromatography. Hoʻohana ʻia hoʻi ia ma ke ʻano he kūlana kaohi kūloko no nā reagents.

Kaʻina Hana Hoʻāʻo:

1. Pono e hōʻiliʻili ʻia nā maʻi me nā hōʻailona. Wahi a nā hōʻike, ʻo ka nui o ka hoʻokuʻu ʻia ʻana o ka rotavirus i loko o nā ʻōpala o nā maʻi me ka gastroenteritis e hana ʻia he 3-5 mau lā ma hope o ka hoʻomaka ʻana o ka maʻi a me 3-13 mau lā ma hope o ka hoʻomaka ʻana o nā hōʻailona. Inā hōʻiliʻili ʻia ka hāpana ma hope o ka maʻi diarrhea, ʻaʻole lawa paha ka nui o nā antigens e hana i ka hopena maikaʻi.

2. Pono e hōʻiliʻili ʻia nā laʻana i loko o kahi pahu maʻemaʻe, maloʻo, a pale wai ʻole i loaʻa ʻole nā ​​​​​​mea holoi a me nā mea mālama.

3. No nā poʻe maʻi ʻaʻole i loaʻa i ka maʻi kahe, ʻaʻole pono e emi iho nā hāpana ʻōpala i hōʻiliʻili ʻia ma mua o 1-2 grams. No nā poʻe maʻi me ka maʻi kahe, inā he wai ka ʻōpala, e ʻoluʻolu e hōʻiliʻili ma ka liʻiliʻi he 1-2 ml o ka wai ʻōpala. Inā he nui ke koko a me ka mucus i loko o ka ʻōpala, e ʻoluʻolu e hōʻiliʻili hou i ka hāpana.

4. Manaʻo ʻia e hoʻāʻo koke i nā laʻana ma hope o ka hōʻiliʻili ʻana, a i ʻole e hoʻouna ʻia i ka hale hana i loko o 6 mau hola a mālama ʻia ma 2-8°C. Inā ʻaʻole i hoʻāʻo ʻia nā laʻana i loko o 72 mau hola, pono e mālama ʻia ma ka mahana ma lalo o -15°C.

5. E hoʻohana i nā ʻōpala hou no ka hoʻāʻo ʻana, a me nā ʻōpala i hui pū ʻia me ka wai hoʻoheheʻe a i ʻole ka wai i hoʻoheheʻe ʻia