ỤKPỤRỤ

A na-ekpuchi akpụkpọ ahụ nke ngwaọrụ nnwale ahụ na mgbochi Tf na mpaghara nnwale ahụ na mgbochi IgG nke ewu na mpaghara njikwa. A na-ekpuchi mpempe akwụkwọ Lable site na iji fluorescence nke a na-akpọ anti Tf antibody na oke bekee IgG tupu oge eruo. Mgbe a na-anwale ihe nlele dị mma, antigen Tf dị na ihe nlele ahụ na-ejikọta ya na mgbochi Tf antibody nke a na-akpọ fluorescence, ma na-emepụta ngwakọta mgbochi. N'okpuru ọrụ nke immunochromatography, usoro mgbagwoju anya na-aga n'ihu nke akwụkwọ na-amịkọrọ mmiri. Mgbe mgbagwoju anya gafere mpaghara nnwale ahụ, ọ jikọtara ya na mgbochi Tf coating antibody, na-emepụta mgbagwoju ọhụrụ.

Ọ bụrụ na ọ dị njọ, ihe nlele ahụ enweghị transferrin ma ọ bụ ọdịnaya ya dị ala, nke mere na a gaghị enwe ike ịmepụta ihe mgbochi ahụ. Agaghị enwe ahịrị uhie na mpaghara nchọpụta (T). Ma ọgwụ mgbochi Tf dị na ihe nlele ahụ ma ọ bụ na ọ dị, ọgwụ mgbochi monoclonal nke òké na-emegide mmadụ IgM monoclonal nke e ji ọla edo dee colloidal na ọgwụ mgbochi òké IgG nke ewu na-egbochi òké nke a kpụchara n'ahịrị njikwa mma (C). Mgbe ahụ, agglutinates na-etolite agba na mpaghara njikwa mma, ahịrị uhie ga-apụta na (C). Ahịrị uhie bụ ọkọlọtọ ahụ na-apụta na mpaghara njikwa mma (C) iji chọpụta ma enwere ihe nlele zuru oke na ma usoro chromatography ọ bụ ihe nkịtị. A na-ejikwa ya dị ka ọkọlọtọ njikwa dị n'ime maka ihe ndị na-eme ihe.

Usoro Ule

Biko gụọ ihe e tinyere na ngwugwu tupu ị nwalee ya.

1. Wepụ kaadị ule ahụ n'ime akpa foil ahụ, tinye ya na tebụl dị larịị wee tinye akara na ya.

2. Wepụ mkpuchi ahụ n'ime ọkpọkọ ihe nlele ahụ ma tụfuo ntapụ abụọ mbụ nke ihe nlele ahụ a gwakọtara agwakọta, tinye ntapụ atọ (ihe dị ka 100uL) nke ihe nlele ahụ a gwakọtara agwakọta nke na-enweghị afụ n'elu ya ma jiri nwayọọ nwayọọ banye n'ime olulu ihe nlele nke kaadị ahụ yana ihe nlele enyere. Mgbe ahụ, malite oge.