MFUNDO

Nembanemba ya chipangizo choyesera imakutidwa ndi antibody ya Tf pamalo oyesera ndipo antibody ya mbuzi ya IgG pamalo olamulira. Mapepala olembera amakutidwa ndi antibody ya fluorescence yolembedwa kuti anti Tf ndi antibody ya kalulu IgG pasadakhale. Poyesa chitsanzo chabwino, antigen ya Tf mu chitsanzo imaphatikizana ndi antibody ya fluorescence yolembedwa kuti anti Tf, ndikupanga chisakanizo cha chitetezo chamthupi. Mothandizidwa ndi immunochromatography, zovuta zimapita molunjika ku pepala loyamwa. Pamene zovuta zimadutsa pamalo oyesera, zimaphatikizana ndi antibody ya anti Tf, zimapanga zovuta zatsopano.

Ngati ili ndi kachilombo koyambitsa matenda, chitsanzocho sichili ndi transferrin kapena kuchuluka kwake kuli kochepa, kotero kuti chitetezo chamthupi sichingapangidwe. Sipadzakhala mzere wofiira m'dera lozindikira (T). Kaya pali Tf antibody mu chitsanzocho kapena ayi, antibody yotsala ya mbewa yotsutsana ndi anthu ya IgM monoclonal yokhala ndi chizindikiro chagolide ndi antibody ya mbuzi yotsutsana ndi mbewa ya IgG yokutidwa mu mzere wowongolera khalidwe (C). Kenako ma agglutinates amapanga mtundu m'dera lowongolera khalidwe, ndipo mzere wofiira udzawonekera mu (C). Mzere wofiira ndi muyezo womwe umawonekera m'dera lowongolera khalidwe (C) kuti udziwe ngati pali zitsanzo zokwanira komanso ngati njira ya chromatography ndi yachibadwa. Imagwiritsidwanso ntchito ngati muyezo wowongolera mkati mwa ma reagents.

Njira Yoyesera

Chonde werengani zomwe zili mu phukusi musanayese.

1. Tulutsani khadi loyesera kuchokera mu thumba la foil, liyikeni patebulo lolingana ndikuliyika chizindikiro.

2. Chotsani chivundikirocho mu chubu cha chitsanzo ndikutaya madontho awiri oyamba a chitsanzo chochepetsedwa, onjezerani madontho atatu (pafupifupi 100uL) opanda thovu lochepetsedwa molunjika komanso pang'onopang'ono mu chitsime cha chitsanzo cha khadi chomwe chili ndi dispette yoperekedwa. Kenako yambani nthawi.