MFUNDO

Chiwalo cha chipangizo choyesera chimakutidwa ndi antigen ya Rotavirus Group A pamalo oyesera ndi antibody ya mbuzi ya IgG yotsutsana ndi kalulu pamalo olamulira. Mapepala olembedwa amakutidwa ndi antibody ya Rotavirus Group A yolembedwa ndi fluorescence ndi IgG ya kalulu pasadakhale. Poyesa chitsanzo chabwino, RV mu chitsanzo imaphatikizana ndi antibody ya Rotavirus Group A yolembedwa ndi fluorescence, ndikupanga chisakanizo cha chitetezo chamthupi. Pansi pa ntchito ya immunochromatography, zovuta zimapita molunjika ku pepala loyamwa. Pamene zovutazo zidadutsa malo oyesera, zimaphatikizana ndi antibody ya anti-Rotavirus Group A, zimapanga chida chatsopano. Ngati zili zoipa, palibe antigen ya Rotavirus Group A mu chitsanzocho, kotero kuti chitetezo chamthupi sichingapangidwe, sipadzakhala mzere wofiira pamalo ozindikira (T). Mosasamala kanthu kuti rotavirus ya gulu A ilipo mu chitsanzocho, mbewa ya IgG yolembedwa ndi latex imayikidwa mu chromatograph ku malo owongolera khalidwe (C) ndikugwidwa ndi antibody ya anti-mbewa ya IgG. Mzere wofiira udzawonekera pamalo owongolera khalidwe (C). Mzere wofiira ndi muyezo womwe umawonekera m'dera lowongolera khalidwe (C) kuti muwone ngati pali zitsanzo zokwanira komanso ngati njira ya chromatography ndi yachibadwa. Umagwiritsidwanso ntchito ngati muyezo wamkati wa ma reagents.

Njira Yoyesera:

1. Odwala omwe ali ndi zizindikiro ayenera kutengedwa. Malinga ndi malipoti, kutuluka kwakukulu kwa rotavirus m'matumbo mwa odwala omwe ali ndi gastroenteritis kumachitika masiku 3-5 kuchokera pamene matendawa adayamba komanso masiku 3-13 kuchokera pamene zizindikiro zayamba. Ngati chitsanzocho chatengedwa nthawi yayitali pambuyo poti kutsegula m'mimba kwachitika, kuchuluka kwa ma antigen sikungakhale kokwanira kuti pakhale zotsatira zabwino.

2. Zitsanzo ziyenera kusonkhanitsidwa mu chidebe choyera, chouma, komanso chosalowa madzi chomwe chilibe sopo ndi zotetezera.

3. Kwa odwala omwe alibe matenda otsegula m'mimba, zitsanzo za ndowe zomwe zasonkhanitsidwa siziyenera kupitirira magalamu 1-2. Kwa odwala omwe ali ndi vuto lotsegula m'mimba, ngati ndowe ndi zamadzimadzi, chonde tengani madzi osachepera 1-2 ml. Ngati ndowe zili ndi magazi ndi ntchofu zambiri, chonde tenganinso chitsanzocho.

4. Ndikoyenera kuyesa zitsanzo nthawi yomweyo mutazitenga, apo ayi ziyenera kutumizidwa ku labotale mkati mwa maola 6 ndikusungidwa pa 2-8°C. Ngati zitsanzozo sizinayesedwe mkati mwa maola 72, ziyenera kusungidwa kutentha komwe kuli pansi pa -15°C.

5. Gwiritsani ntchito ndowe zatsopano poyesa, ndi zitsanzo za ndowe zosakaniza ndi madzi osungunuka kapena osungunuka