NHEYO

Kamudzira kemudziyo wekuyedza kakaputirwa neTf antibody panzvimbo yekuyedza uye mbudzi anti rabbit IgG antibody panzvimbo yekudzora. Mapepa eLable pad akaputirwa ne fluorescence yakanyorwa kuti anti Tf antibody uye tsuro IgG pachine nguva. Pakuyedza positive sample, Tf antigen iri mumuenzaniso inosangana ne fluorescence yakanyorwa kuti anti Tf antibody, uye inoumba immune immune mixture. Pasi pekushanda kwe immunochromatography, complex inoyerera ichienda kune bepa rinonyudza. Kana complex yapfuura nzvimbo yekuyedza, yakabatana ne anti Tf coating antibody, inoumba complex itsva.

Kana iri negative, sampuro haina transferrin kana kuti zvirimo zvakaderera, zvekuti immune complex haigone kuumbwa. Hapazovi nemutsara mutsvuku munzvimbo yekuona (T). Hazvina mhosva kuti Tf antibody iripo here musampuli kana kuti kwete, yasara colloidal gold-labeled gold anti-human IgM monoclonal antibody uye goat anti-mouse IgG antibody zvakaputirwa mumutsara wenzvimbo yekudzora mhando (C). Zvadaro ma agglutinates anova neruvara munzvimbo yekudzora mhando, uye mutsetse mutsvuku uchaonekwa mu (C). Mutsetse mutsvuku ndiwo mwero unoonekwa munzvimbo yekudzora mhando (C) wekutonga kana paine samples dzakakwana uye kana maitiro e chromatography ari akajairwa. Inoshandiswawo semwero wemukati we reagents.

Maitiro Ekuyedza

Ndapota verengai zviri mupakeji musati maedza.

1. Bvisa kadhi rekuyera kubva mubhegi refoil, riise patafura yakaenzana worimaka.

2. Bvisa chivharo kubva pachubhu yemuenzaniso worasa madonhwe maviri ekutanga akasanganiswa, wedzera madonhwe matatu (anenge 100uL) pasina bubble yakasanganiswa yakatwasuka yakatwasuka uye zvishoma nezvishoma mutsime remuenzaniso wekadhi rine dispette yakapihwa. Wobva watanga timer.