KA'IDA

An shafa membrane na na'urar gwajin da maganin rigakafi na Tf a yankin gwaji da kuma maganin rigakafi na IgG na maganin akuya a yankin sarrafawa. Ana shafa murfin lable da sinadarin fluorescence mai lakabin anti Tf antibody da kuma maganin IgG na zomo a gaba. Lokacin gwada samfurin tabbatacce, maganin rigakafi na Tf a cikin samfurin yana haɗuwa da maganin rigakafi na Tf mai lakabin fluorescence, kuma yana samar da cakuda garkuwar jiki. A ƙarƙashin aikin immunochromatography, kwararar hadaddun zuwa ga takardar sha. Lokacin da hadaddun ya wuce yankin gwaji, ya haɗu da maganin rigakafi na anti Tf, yana samar da sabon hadaddun.

Idan ba shi da kyau, samfurin ba ya ɗauke da transferrin ko kuma abun da ke ciki ya yi ƙasa, don haka ba za a iya samar da hadaddun garkuwar jiki ba. Ba za a sami layin ja a yankin ganowa (T) ba. Ko akwai maganin rigakafi na Tf a cikin samfurin ko a'a, sauran maganin rigakafi na IgM monoclonal na linzamin kwamfuta mai alamar zinare da aka yi wa lakabi da colloidal da kuma maganin rigakafi na IgG na akuya da aka shafa a layin yankin kula da inganci (C). Sannan agglutinates suna samun launi a yankin kula da inganci, kuma layin ja zai bayyana a (C). Layin ja shine ma'aunin da ke bayyana a yankin kula da inganci (C) don tantance ko akwai isassun samfura da kuma ko tsarin chromatography na al'ada ne. Haka kuma ana amfani da shi azaman ma'aunin kula da ciki don abubuwan da ke haifar da sinadarai.

Tsarin Gwaji

Da fatan za a karanta abin da aka saka a cikin fakitin kafin a gwada.

1. Cire katin gwaji daga jakar foil, sanya shi a kan teburin matakin sannan ka yi masa alama.

2. Cire murfin daga bututun samfurin kuma jefar da digo biyu na farko na samfurin da aka narkar, ƙara digo 3 (kimanin 100uL) ba tare da kumfa ba a tsaye da hankali a cikin rijiyar samfurin na katin tare da dispette da aka bayar. Sannan kunna mai ƙidayar lokaci.