TE MATUA

Kua pania te kiriuhi o te taputapu whakamātautau ki te paturopi Tf i te rohe whakamātautau, me te paturopi IgG koati anti-rāpeti i te rohe whakahaere. Kua pania te papa tapanga ki te paturopi anti-Tf me te IgG rāpeti kua tohua ki te fluorescence i mua i te whakamātautau. I te whakamātautau i te tauira pai, ka honoa te antigen Tf i roto i te tauira ki te paturopi anti-Tf kua tohua ki te fluorescence, ka hanga he ranunga ārai mate. I raro i te mahi a te immunochromatography, ka rere te matatini i te ahunga o te pepa mimiti. Ina paahitia e te matatini te rohe whakamātautau, ka honoa ki te paturopi paninga anti-Tf, ka hanga he matatini hou.

Mena he kino, kāore he transferrin i roto i te tauira, he iti rānei te ihirangi, ā, kāore e taea te hanga i te matatini ārai mate. Kāore he rārangi whero i te rohe kitenga (T). Ahakoa kei roto i te tauira te paturopi Tf, ko te toenga o te paturopi monoclonal anti-human IgM kiore kua tohua ki te koura me te paturopi anti-mouse IgG koati kua pania ki te rārangi rohe whakahaere kounga (C). Kātahi ka puta he tae i roto i te rohe whakahaere kounga, ā, ka puta te rārangi whero i roto i te (C). Ko te rārangi whero te paerewa e puta ana i roto i te rohe whakahaere kounga (C) hei whakatau mena he nui ngā tauira, me te mea he noa te tukanga chromatography. Ka whakamahia hoki hei paerewa whakahaere ā-roto mō ngā matū.

Tukanga Whakamātautau

Pānuihia te tāpiringa ki te kete i mua i te whakamātautau.

1. Tangohia te kāri whakamātautau mai i te putea pepa konutea, whakatakotoria ki runga i te tēpu papatahi, ka tohua.

2. Tangohia te taupoki i te ngongo tauira, ka maka atu ngā pata tuatahi e rua o te tauira kua whakaranua, tāpirihia kia 3 ngā pata (tata ki te 100µL) o te tauira kua whakaranua, kia poutū, kia āta haere ki roto i te poka tauira o te kāri me te dispette kua whakaratohia. Kātahi ka tīmata te taima.