Zida Zodziwira Matenda a Vitamini D wa 25-hydroxy(kuyesa kwa fluorescence immunochromatographic)
Kugwiritsa ntchito poyezetsa matenda m'thupi kokha
Chonde werengani bwino phukusili musanagwiritse ntchito ndipo tsatirani malangizo mosamala. Kudalirika kwa zotsatira za mayeso sikungatsimikizidwe ngati pali kusiyana kulikonse kuchokera ku malangizo omwe ali mu phukusili.

KUGWIRITSA NTCHITO KOFUNIKA
Chida Chodziwira Matenda cha 25-hydroxy Vitamini D (fluorescence immunochromatographic assay) ndi njira yodziwira kuchuluka kwa 25-hydroxy Vitamini D (25-(OH)VD) mu seramu ya anthu kapena plasma, yomwe imagwiritsidwa ntchito makamaka poyesa kuchuluka kwa vitamini D. Ndi reagent yothandizira kuzindikira matenda. Zitsanzo zonse zabwino ziyenera kutsimikiziridwa ndi njira zina. Mayesowa ndi a akatswiri azaumoyo okha.

CHIDULE
Vitamini D ndi vitamini ndipo ndi hormone ya steroid, makamaka kuphatikiza VD2 ndi VD3, yomwe kapangidwe kake kamafanana kwambiri. Vitamini D3 ndi D2 amasinthidwa kukhala 25 hydroxyl vitamini D (kuphatikiza 25-dihydroxyl vitamini D3 ndi D2). 25-(OH) VD m'thupi la munthu, kapangidwe kokhazikika, kuchuluka kwakukulu. 25-(OH) VD imasonyeza kuchuluka kwa vitamini D, komanso mphamvu ya kusintha kwa vitamini D, kotero 25-(OH)VD imaonedwa kuti ndi chizindikiro chabwino kwambiri chowunikira kuchuluka kwa vitamini D. Diagnostic Kit imachokera pa immunochromatography ndipo ingapereke zotsatira mkati mwa mphindi 15.

MFUNDO YA NJIRAYI
Nembanemba ya chipangizo choyesera imakutidwa ndi conjugate ya BSA ndi 25-(OH)VD pamalo oyesera ndi antibody ya mbuzi ya IgG pamalo olamulira. Mapepala olembera amakutidwa ndi antibody ya fluorescence mark anti 25-(OH)VD ndi antibody ya kalulu IgG pasadakhale. Poyesa chitsanzo, 25-(OH)VD mu chitsanzo imaphatikizana ndi antibody ya fluorescence mark antibody ya 25-(OH)VD, ndikupanga chisakanizo cha chitetezo chamthupi. Pogwiritsa ntchito immunochromatography, kuyenda kwa zovuta kumayang'ana pepala loyamwa, pamene zovutazo zadutsa pamalo oyesera, chizindikiro cha fluorescent chaulere chidzaphatikizidwa ndi 25-(OH)VD pa nembanemba. Kuchuluka kwa 25-(OH)VD ndi mgwirizano woipa wa chizindikiro cha fluorescence, ndipo kuchuluka kwa 25-(OH)VD mu chitsanzo kumatha kuzindikirika ndi fluorescence immunoassay assay.

ZOPANGIRA NDI ZIPANGIZO ZOPEREKEDWA

Zigawo za phukusi la 25T：
Khadi loyesera lokhala ndi foil payokha lokhala ndi desiccant 25T
Yankho la .A 25T
Yankho la .B 1
. Phukusi lolowera 1

Zipangizo zofunika koma siziperekedwa
Chidebe chosonkhanitsira zitsanzo, nthawi

Zitsanzo Zosonkhanitsira ndi Kusunga
1. Zitsanzo zomwe zayesedwa zitha kukhala seramu, heparin anticoagulant plasma kapena EDTA anticoagulant plasma.
2. Kutengera ndi njira zodziwika bwino, sonkhanitsani chitsanzo. Seramu kapena plasma chitsanzo chikhoza kusungidwa mufiriji pa 2-8℃ kwa masiku 7 ndikusungidwa pansi pa -15°C kwa miyezi 6.
3. Zitsanzo zonse kupewa kuzizira ndi kusungunula madzi.

NJIRA YOYESERA
Njira yoyesera chipangizochi onani buku la immunoanalyzer. Njira yoyesera reagent ndi iyi:
1. Ikani pambali zinthu zonse zoyeretsera ndi zitsanzo kutentha kwa chipinda.
2. Tsegulani Portable Immune Analyzer (WIZ-A101), lowetsani mawu achinsinsi olowera mu akaunti malinga ndi njira yogwirira ntchito ya chidacho, ndikulowetsa mawonekedwe ozindikira.
3.Scan code yodziwitsa kuti mutsimikizire chinthu choyesera.
4. Tulutsani khadi loyesera m'thumba la foil.
5. Ikani khadi loyesera mu malo oikamo khadi, sikani QR code, ndikuzindikira chinthu choyesera.
6. Onjezani 30μL seramu kapena chitsanzo cha plasma mu yankho la A, ndipo sakanizani bwino.
7. Onjezani yankho la 50μL B ku chisakanizo chomwe chili pamwambapa, ndipo sakanizani bwino.
8.Siyani chisakanizocho kwa mphindi 15.
9. Onjezani 80μL yosakaniza kuti muyesere chitsime cha khadi.
10. Dinani batani la "standard test", patatha mphindi 10, chidacho chidzazindikira khadi loyesera lokha, chimatha kuwerenga zotsatira kuchokera pazenera lowonetsera chidacho, ndikulemba/kusindikiza zotsatira za mayeso.
11. Onani malangizo a Portable Immune Analyzer (WIZ-A101).

MFUNDO ZOYEMBEKEZEDWA
25-(OH)VD mulingo wabwinobwino: 30-100ng/mL
Ndikofunikira kuti labotale iliyonse ikhazikitse kuchuluka kwake komwe kumayimira odwala ake.

Zotsatira za Mayeso ndi Kutanthauzira
Deta yomwe ili pamwambapa ndi nthawi yowunikira yomwe idakhazikitsidwa kuti ipeze deta ya zida izi, ndipo akulangizidwa kuti labotale iliyonse ikhazikitse nthawi yowunikira kufunika kwachipatala kwa anthu m'derali.
Kuchuluka kwa 25-(OH)VD ndi kwakukulu kuposa kuchuluka kwa zizindikiro, ndipo kusintha kwa thupi kapena kuyankha kwa kupsinjika kuyenera kuchotsedwa. Zoonadi, si zachilendo, ziyenera kuphatikiza kuzindikira zizindikiro zachipatala.
Zotsatira za njira iyi zimagwira ntchito kokha pa mndandanda wa zofotokozera zomwe zakhazikitsidwa ndi njira iyi, ndipo zotsatira zake sizingafanane mwachindunji ndi njira zina.
Zinthu zina zingayambitsenso zolakwika pakupeza zotsatira, kuphatikizapo zifukwa zaukadaulo, zolakwika pakugwira ntchito ndi zinthu zina zachitsanzo.

KUSUNGA NDI KUKHALA BWINO
.Zidazi zimatha kusungidwa kwa miyezi 18 kuyambira tsiku lopangidwa. Sungani zida zomwe sizinagwiritsidwe ntchito pa kutentha kwa 2-30°C. MUSAZIYIKE MU MAFURI. Musagwiritse ntchito kupitirira tsiku lotha ntchito.
Musatsegule thumba lotsekedwa mpaka mutakonzeka kuchita mayeso, ndipo mayeso ogwiritsidwa ntchito kamodzi akulangizidwa kuti agwiritsidwe ntchito pamalo ofunikira (kutentha 2-35℃, chinyezi 40-90%) mkati mwa mphindi 60 mwachangu momwe mungathere.
Chitsanzo cha diluent chimagwiritsidwa ntchito nthawi yomweyo chikatsegulidwa.

MACHENJEZO NDI ZOCHENJEZERA
.Zida ziyenera kutsekedwa ndi kutetezedwa ku chinyezi.
Zitsanzo zonse zabwino ziyenera kutsimikiziridwa ndi njira zina.
Zitsanzo zonse ziyenera kuonedwa ngati zowononga chilengedwe.
MUSAGWIRITSE NTCHITO reagent yotha ntchito.
MUSASINTHIRE ma reagent pakati pa zida zomwe zili ndi nambala yosiyana.
MUSAGWIRITSE NTCHITO makhadi oyesera ndi zinthu zina zilizonse zogwiritsidwa ntchito ngati zinyalala.
Kugwiritsa ntchito molakwika, kuchuluka kwa zitsanzo kapena zochepa kungayambitse kusintha kwa zotsatira.

LKUTSANZIRA
Monga momwe zimakhalira ndi mayeso aliwonse omwe amagwiritsa ntchito ma antibodies a mbewa, pali kuthekera kwa kusokonezedwa ndi ma antibodies a anti-mbewa a anthu (HAMA) mu chitsanzocho. Zitsanzo kuchokera kwa odwala omwe adalandira ma antibodies a monoclonal kuti apeze matenda kapena chithandizo zitha kukhala ndi HAMA. Zitsanzo zotere zingayambitse zotsatira zabodza kapena zabodza.
Zotsatira za mayesowa ndi za chithandizo chamankhwala chokha, siziyenera kukhala maziko okhawo odziwira matenda ndi chithandizo chamankhwala, kuyang'anira odwala kuyenera kuganiziridwa mokwanira pamodzi ndi zizindikiro zake, mbiri yachipatala, kufufuza kwina kwa labotale, yankho la chithandizo, matenda a epidemiology ndi zina zambiri.
Chogwiritsiridwa ntchitochi chimagwiritsidwa ntchito poyesa seramu ndi plasma yokha. Sichingapeze zotsatira zolondola chikagwiritsidwa ntchito pa zitsanzo zina monga malovu ndi mkodzo ndi zina zotero.

MAKHALIDWE A NTCHITO

RMFUNDO ZOFUNIKA

1.Hansen JH, ndi ena. Kusokoneza kwa HAMA ndi Murine Monoclonal Antibody-Based Immunoassays [J].J wa Clin Immunoassay,1993,16:294-299.
2.Levinson SS. Mtundu wa Ma Antibodies Osatha ndi Udindo mu Kusokoneza kwa Immunoassay [J].J wa Clin Immunoassay,1992,15:108-114.

Chinsinsi cha zizindikiro zomwe zagwiritsidwa ntchito:

	Chipangizo Chachipatala Chodziwitsira Matenda mu Vitro
	Wopanga
	Sungani pamalo otentha 2-30℃
	Tsiku lothera ntchito
	Musagwiritsenso ntchito
	CHENJEZO
	Onani Malangizo Ogwiritsira Ntchito

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