IHAME

Agace k'igikoresho cyo gupima gatwikiriwe na antibody ya Tf ku gace k'igeragezwa naho antibody ya IgG yo mu ihene itwikiriwe na antibody ya IgG yo mu rukwavu ku gace k'igenzura. Udupapuro two gupima dutwikiriwe na antibody ya Tf ya fluorescence na antibody ya IgG yo mu rukwavu mbere y'igihe. Mu gihe cyo gupima icyitegererezo cyagaragaye, antigen ya Tf iri mu cyitegererezo ihuzwa na antibody ya Tf ya fluorescence, maze igakora uruvange rw'ubudahangarwa bw'umubiri. Mu gihe cy'imikorere ya immunochromatography, urusobe rw'imitsi runyura mu cyerekezo cy'impapuro zifata. Iyo complex inyuze mu gace k'igerageza, yahujwe na antibody ya Tf yo gutwikira, ikora complex nshya.

Iyo ari negative, icyitegererezo ntikiba kirimo transferrin cyangwa ibikubiye mu gipimo biri hasi, ku buryo ubudahangarwa bw'umubiri budashobora kubaho. Nta murongo utukura uzaba uri mu gace ko gupima (T). Uko antibody ya Tf yaba iri muri specimen cyangwa idafite, antibody yasigaye ya IgM monoclonal ya mbeba ifite label ya gold-labeled gold-labeled goat-antibody ya IgG yatwikiriwe mu gace ko kugenzura ubuziranenge (C). Hanyuma agglutinates zikura ibara mu gace ko kugenzura ubuziranenge, kandi umurongo utukura uzagaragara muri (C). Umurongo utukura ni wo ugaragara mu gace ko kugenzura ubuziranenge (C) kugira ngo harebwe niba hari ingero zihagije kandi niba inzira ya chromatography ari isanzwe. Ikoreshwa kandi nk'igipimo cy'igenzura ry'imbere ku bipimo.

Uburyo bwo gupima

Mbere yo gupima, soma agasanduku kari ku rupapuro.

1. Kura ikarita yo gupima mu gikapu cya foil, uyishyire ku meza iringaniye hanyuma uyishyireho akamenyetso.

2. Kuraho umupfundikizo mu muyoboro w'icyitegererezo hanyuma ujugunye ibitonyanga bibiri bya mbere byakuwemo amazi, ongeramo ibitonyanga bitatu (hafi 100uL) nta bitonyanga byakuwemo amazi bihagaze buhoro buhoro mu mwobo w'ikarita ufite dispette yatanzwe. Hanyuma utangire igihe.