Kiti yekuongorora ye25-hydroxy Vitamin D(kuyedza kwe fluorescence immunochromatographic)
Kushandiswa kwekuongorora in vitro chete
Ndapota verengai zviri mupakeji iyi nokungwarira musati mashandisa uye teverai mirairo yacho nemazvo. Kuvimbika kwemhedzisiro yekuyedza hakugone kuvimbiswa kana paine chero kutsauka kubva pamirairo iri mupakeji iyi.

KUSHANDISA KWAKATARISIRWA
Diagnostic Kit ye25-hydroxy Vitamin D (fluorescence immunochromatographic assay) inzira yekuongorora 25-hydroxy Vitamin D (25-(OH)VD) muropa revanhu kana muplasma, iyo inonyanya kushandiswa kuongorora huwandu hwevhitamini D. Iyi inzira yekubatsira pakuongorora chirwere. Sampuli dzese dzakanaka dzinofanira kusimbiswa nedzimwe nzira. Bvunzo iyi yakagadzirirwa kushandiswa nenyanzvi dzezvehutano chete.

PFUPISO
Vhitamini D ivhitamini uye zvakare ihormone yesteroid, kunyanya inosanganisira VD2 neVD3, ine chimiro chakafanana zvikuru. Vhitamini D3 neD2 zvinoshandurwa kuita 25 hydroxyl vitamin D (kusanganisira 25-dihydroxyl vitamin D3 neD2). 25-(OH) VD mumuviri wemunhu, chimiro chakasimba, huwandu hwakawanda. 25-(OH) VD inoratidza huwandu hwese hwevhitamini D, uye kugona kwekushandura kwevhitamini D, saka 25-(OH)VD inoonekwa sechiratidzo chakanakisa chekuongorora huwandu hwevhitamini D. Diagnostic Kit yakavakirwa pa immunochromatography uye inogona kupa mhedzisiro mukati memaminitsi gumi nemashanu.

NZVIMBO YEMASHURE
Kamudzira kemudziyo wekuyedza kakaputirwa neBSA ne25-(OH)VD panzvimbo yekuyedza uye antibody yembudzi yeIgG antibody iri panzvimbo yekudzora. Mapedhi emarker anoputirwa neantibody yefluorescence mark anti25-(OH)VD uye antibody yetsuro IgG pachine nguva. Pakuyedza sampuro, 25-(OH)VD mumuenzaniso inosanganiswa neantibody yefluorescence mark anti25-(OH)VD, uye inoumba musanganiswa we immune. Pasi pekushanda kwe immunochromatography, kuyerera kwakaomarara kunoenda kune bepa rinonyudza, kana complex yapfuura nzvimbo yekuyedza, chiratidzo chefree fluorescent chichabatanidzwa ne25-(OH)VD pamembrane. Kuwanda kwe25-(OH)VD inyaya ye negative correlation ye fluorescence signal, uye kuwanda kwe25-(OH)VD mumuenzaniso kunogona kuonekwa ne fluorescence immunoassay assay.

ZVINHU ZVINOGONESESWA NEZVINHU ZVINOPIWA

Zvikamu zvepakeji zve25T：
Kadhi rekuyedza rakaiswa mufoil imwe neimwe ine desiccant 25T
Mhinduro 25T
Mhinduro ye.B 1
.Pakeji yekuisa 1

Zvinhu zvinodiwa asi hazvipihwe
Mudziyo wekuunganidza wemuenzaniso, nguva

MIFANANIDZO YEKUCHENGETA NOKUCHENGETA
1. Mienzaniso yakaedzwa inogona kuva serum, heparin anticoagulant plasma kana EDTA anticoagulant plasma.
2. Zvinoenderana nemaitiro akajairwa, unganidzai sampuro. Serum kana plasma sampuro inogona kuchengetwa mufiriji pa2-8℃ kwemazuva manomwe uye cryopreservation iri pasi pe -15°C kwemwedzi mitanhatu.
3.Sarudzo dzese dzinodzivisa kutonhora nekunyunguduka.

MAITIRO EKUONGORORA
Maitiro ekuyedza mudziyo ona bhuku re immunoanalyzer. Maitiro ekuyedza reagent ndeekuti:
1. Isa parutivi ma reagents ese nemasamples patembiricha yemukamuri.
2.Vhura Portable Immune Analyzer(WIZ-A101), isa password yekupinda muakaunti zvichienderana nenzira yekushanda kwemudziyo, uye isa interface yekuona.
3. Skena kodhi yekuziva kuti usimbise chinhu chebvunzo.
4. Bvisa kadhi rekuyedza kubva mubhegi refoil.
5. Isa kadhi rekuyera mubhokisi remakadhi, skena QR code, uye sarudza chinhu chekuyedza.
6. Wedzera 30μL serum kana plasma sample muA solution, wosanganisa zvakanaka.
7. Wedzera 50μL B solution kumusanganiswa uri pamusoro apa, wosanganisa zvakanaka.
8.Siya musanganiswa kwemaminitsi gumi nemashanu.
9. Wedzera musanganiswa we80μL kuti uedze tsime rekadhi.
10. Dzvanya bhatani rekuti "standard test", mushure memaminitsi gumi, mudziyo unoona kadhi rebvunzo otomatiki, unogona kuverenga mhinduro kubva pachiratidziro chemudziyo, uye kunyora/kudhinda mhinduro dzebvunzo.
11. Tarisa mirairo yePortable Immune Analyzer (WIZ-A101).

ZVINHU ZVINOTARISIRWA
25-(OH)VD yakajairika nhanho: 30-100ng/mL
Zvinokurudzirwa kuti rabhoritari yega yega igadzire huwandu hwayo hwevarwere vayo.

Mhedzisiro yeMiedzo neDudziro
.Data iri pamusoro apa ndiyo nguva yekutarisa data rekuona rekit iyi, uye zvinokurudzirwa kuti rabhoritari yega yega inofanira kugadzira nguva yekutarisa kukosha kwekiriniki kwevanhu vari munharaunda iyi.
.Kuwanda kwe25-(OH)VD kwakakwira kupfuura huwandu hwezviratidzo, uye shanduko dzemuviri kana mhinduro yekushushikana hazvifanirwe kubviswa. Zvechokwadi, kunofanira kusanganisa kuongororwa kwezviratidzo zvekiriniki.
.Mhedzisiro yenzira iyi inoshanda chete kune huwandu hwemareferensi hwakatarwa nenzira iyi, uye mhedzisiro yacho haifananidzwe zvakananga nedzimwe nzira.
Zvimwe zvinhu zvinogona kukonzera zvikanganiso mumhedzisiro yekuona, kusanganisira zvikonzero zvehunyanzvi, zvikanganiso zvekushanda uye zvimwe zvinhu zvemuenzaniso.

KUCHENGETA UYE KUGADZIRIKA
Kiti iyi inogara kwemwedzi gumi nemisere kubva pazuva rakagadzirwa. Chengeta makiti asina kushandiswa pa2-30°C. USAIISA muchando. Usashandise mushure mezuva rekupera.
Usavhura homwe yakavharwa kusvika wagadzirira kuita bvunzo, uye bvunzo inoshandiswa kamwe chete inokurudzirwa kuti ishandiswe munzvimbo inodiwa (tembiricha 2-35℃, hunyoro 40-90%) mukati memaminitsi makumi matanhatu nekukurumidza sezvinobvira.
Muenzaniso wemusanganiswa unoshandiswa pakarepo mushure mekuvhurwa.

YAMBIRO NEMADZIRIRO
.Kit yacho inofanira kuvharwa uye kudzivirirwa kubva kuunyoro.
.Mienzaniso yese yakanaka inofanira kusimbiswa nedzimwe nzira.
.Mienzaniso yese inofanira kubatwa seinogona kusvibisa.
.USASHANDISE reagent yapera basa.
.USACHINJANA ma reagents pakati pemakiti ane nhamba dzakasiyana dzemakit..
.USASHANDISA makadhi ekuyedza uye chero zvishongedzo zvinoraswa.
Kusashanda zvakanaka, kuwanda kwemuenzaniso kana kuti kushoma kunogona kukonzera kutsauka kwemhedzisiro.

LKUTEVEDZA
.Sezvinoita chero bvunzo inoshandisa ma antibodies embeva, pane mukana wekuti ma antibodies emunhu anodzivirira mbeva (HAMA) apindire mumuenzaniso. Ma sampuli kubva kuvarwere vakagamuchira ma antibodies e monoclonal kuti vaongororwe kana kurapwa anogona kunge aine HAMA. Ma sampuli akadaro anogona kukonzera mhedzisiro yenhema kana yenhema.
.Mhedzisiro iyi yebvunzo ndeyekutarisa kukiriniki chete, haifanirwe kushanda senzira chete yekuongororwa kwekiriniki uye kurapwa, manejimendi ekiriniki evarwere anofanira kufungwa zvakadzama pamwe chete nezviratidzo zvavo, nhoroondo yekurapa, kumwe kuongororwa kwerabhoritari, mhinduro yekurapa, epidemiology nedzimwe ruzivo.
.Chishandiso ichi chinoshandiswa chete pakuongororwa kweropa neropa. Chingasawana mhinduro chaiyo kana chikashandiswa kune mamwe masampuli akadai semate neweti nezvimwewo.

Hunhu hweKuita

RZVINOTAURWA

1.Hansen JH, et al.HAMA Kupindirana neMurine Monoclonal Antibody-Based Immunoassays[J].J yeClin Immunoassay,1993,16:294-299.
2.Levinson SS. Hunhu hweHeterophilic Antibodies uye Basa muImmunoassay Interference[J].J yeClin Immunoassay,1992,15:108-114.

Kiyi yezviratidzo zvakashandiswa:

	Mudziyo wekurapa weIn Vitro Diagnostic
	Mugadziri
	Chengeta pa 2-30℃
	Zuva rekupera
	Usashandisezve
	CHENJERERO
	Bvunza Mirayiridzo Yekushandisa

Xiamen Wiz Biotech CO.,LTD
Kero: 3-4 Floor, NO.16 Building, Bio-medical Workshop, 2030 Wengjiao West Road, Haicang District, 361026, Xiamen, China
Nhare:+86-592-6808278
Fakisi:+86-592-6808279