Ikhithi yokuxilonga ye-25-hydroxy Vitamin D(uvavanyo lwe-fluorescence immunochromatographic)
Ukusetyenziswa kokuxilongwa kwi-vitro kuphela
Nceda ufunde le phakheji ngononophelo phambi kokuba uyisebenzise kwaye ulandele imiyalelo ngokungqongqo. Ukuthembeka kweziphumo zovavanyo akunakuqinisekiswa ukuba kukho naluphi na uphambuko kwimiyalelo ekwesi sithuba sephakheji.

UKUSETYENZISWA OKUJOLISWEYO
Isixhobo sokuxilonga se-25-hydroxy Vitamin D (uvavanyo lwe-fluorescence immunochromatographic) luvavanyo lwe-fluorescence immunochromatographic lokubona ubungakanani be-25-hydroxy Vitamin D (25-(OH)VD) kwi-serum okanye kwi-plasma yomntu, esetyenziswa kakhulu ukuvavanya amanqanaba e-vitamin D. Sisixhobo sokuncedisa ukuxilongwa. Zonke iisampulu ezilungileyo kufuneka ziqinisekiswe zezinye iindlela. Olu vavanyo lwenzelwe ukusetyenziswa ziingcali zonyango kuphela.

ISISHWANKATHELO
IVithamini D yivithamini kwaye ikwayi-hormone ye-steroid, ikakhulu equka i-VD2 kunye ne-VD3, eyakhiwe ngendlela efanayo kakhulu. IVithamini D3 kunye ne-D2 ziguqulwa zibe yi-25 hydroxyl vitamin D (kuquka i-25-dihydroxyl vitamin D3 kunye ne-D2). I-25-(OH) VD emzimbeni womntu, ulwakhiwo oluzinzileyo, uxinaniso oluphezulu. I-25-(OH) VD ibonisa isixa siphela sevithamini D, kunye nokukwazi ukuguqula ivithamin D, ngoko ke i-25-(OH)VD ithathwa njengeyona nto ibalaseleyo yokuvavanya inqanaba levithamin D. I-Diagnostic Kit isekelwe kwi-immunochromatography kwaye inokunika isiphumo kwimizuzu eli-15.

UMGAQO WENKQUBO
I-membrane yesixhobo sovavanyo igqunywe nge-conjugate ye-BSA kunye ne-25-(OH)VD kwindawo yovavanyo kunye ne-antibody yebhokhwe ye-IgG antibody kwindawo yokulawula. I-Marker pad igqunywe nge-antibody ye-fluorescence mark anti-25-(OH)VD kunye ne-IgG yomvundla kwangaphambili. Xa kuvavanywa isampuli, i-25-(OH)VD kwisampuli idityaniswa ne-antibody ye-fluorescence mark anti-25-(OH)VD, kwaye yenze umxube wokuzikhusela. Phantsi kwesenzo se-immunochromatography, ukuhamba okuntsonkothileyo kwicala lephepha elifunxayo, xa i-complex idlule kwindawo yovavanyo, i-free fluorescent marker iya kudityaniswa ne-25-(OH)VD kwi-membrane. Uxinzelelo lwe-25-(OH)VD lulwalamano olubi lwesignali ye-fluorescence, kwaye uxinzelelo lwe-25-(OH)VD kwisampuli lunokubonwa ngovavanyo lwe-fluorescence immunoassay.

IZINTO EZINIKEZELWAYO NEZIXHOBO

Izixhobo zephakheji ze-25T：
Ikhadi lovavanyo ngalinye lifakwe ifoyile ngesixhobo sokucoca i-desiccant esingu-25T
Isisombululo se-.A 25T
Isisombululo se-.B 1
.Iphakheji yokufaka 1

IZIXHOBO EZIFUNEKAYO KODWA AZINIKELWANGA
Isikhongozeli sokuqokelela isampuli, isibali-xesha

IMFUNDO YOKUQOKELELA NOKUGCINA IMIZEKELO
1.Iisampulu ezivavanyiweyo zinokuba yi-serum, i-heparin anticoagulant plasma okanye i-EDTA anticoagulant plasma.
2. Ngokweendlela eziqhelekileyo zokuqokelela isampuli. Isampuli ye-serum okanye yeplasma ingagcinwa efrijini kwi-2-8℃ iintsuku ezisi-7 kwaye igcinwe ngaphantsi kwe-15°C iinyanga ezi-6.
3.Zonke iisampuli ziphephe imijikelo yokuqandisa nokunyibilikisa.

INKQUBO YOVAVANYO
Inkqubo yovavanyo lwesixhobo jonga incwadi yemiyalelo ye-immunoanalyzer. Inkqubo yovavanyo lwe-reagent yile ilandelayo
1. Beka ecaleni zonke ii-reagents kunye neesampuli ukuya kubushushu begumbi.
2.Vula i-Portable Immune Analyzer (WIZ-A101), faka igama lokugqitha le-akhawunti ngokwendlela yokusebenza kwesixhobo, uze ufake i-interface yokubona.
3.Skena ikhowudi yokuchonga ukuze uqinisekise into yovavanyo.
4.Khupha ikhadi lovavanyo kwingxowa yefoyile.
5.Faka ikhadi lovavanyo kwindawo yokubeka ikhadi, skena ikhowudi ye-QR, uze umisele into yovavanyo.
6. Yongeza isampuli yeserum okanye iplasma engama-30μL kwisisombululo se-A, uze udibanise kakuhle.
7. Yongeza isisombululo se-50μL B kumxube ongentla, uze udibanise kakuhle.
8.Shiya umxube imizuzu eli-15.
9. Yongeza umxube we-80μL ukuze ufumane isampuli yekhadi.
10. Cofa iqhosha elithi "uvavanyo oluqhelekileyo", emva kwemizuzu eli-10, isixhobo siza kuzibona ngokuzenzekelayo ikhadi lovavanyo, singafunda iziphumo kwisikrini sokubonisa isixhobo, size sirekhode/siprinte iziphumo zovavanyo.
11. Jonga imiyalelo ye-Portable Immune Analyzer (WIZ-A101).

IINQOBO EZILINDELEKILEYO
25-(OH)VD uluhlu oluqhelekileyo: 30-100ng/mL
Kucetyiswa ukuba ilabhoratri nganye imisele uluhlu lwayo oluqhelekileyo olumele inani lezigulana zayo.

IZIPHUMO ZOVAVANYO NOKUTOLIKWA
.Idatha engentla lixesha elimiselweyo lokufumana idatha yale khithi, kwaye kucetyiswa ukuba ilabhoratri nganye imisele ixesha elimiselweyo lokubaluleka kweklinikhi okufanelekileyo kubemi abakulo mmandla.
.Uxinzelelo lwe-25-(OH)VD luphezulu kunoluhlu oluchaziweyo, kwaye utshintsho lwe-physiological okanye impendulo yoxinzelelo kufuneka lungabandakanywa. Enyanisweni, okungaqhelekanga, kufuneka kudityaniswe ukuxilongwa kweempawu zeklinikhi.
Iziphumo zale ndlela zisebenza kuphela kuluhlu lwesalathiso olumiselwe yile ndlela, kwaye iziphumo azifani ngokuthe ngqo nezinye iindlela.
Ezinye izinto zinokubangela iimpazamo kwiziphumo zokufumanisa, kuquka izizathu zobugcisa, iimpazamo zokusebenza kunye nezinye izinto zesampulu.

UKUGCINWA NOKUZINZISA
.Le khithi ihlala iinyanga ezili-18 ukususela kumhla wokwenziwa kwayo. Gcina ezi khithi zingasetyenziswanga kwi-2-30°C. SUKUZIQANDA. Musa ukuzisebenzisa emva komhla wokuphelelwa.
.Musa ukuvula isikhwama esivaliweyo ude ulungele ukwenza uvavanyo, kwaye uvavanyo olusetyenziswa kanye lucetyiswa ukuba lusetyenziswe phantsi kweemeko ezifunekayo (ubushushu obuyi-2-35℃, umswakama oyi-40-90%) kwimizuzu engama-60 ngokukhawuleza.
Isampulu yomxubi isetyenziswa kwangoko emva kokuba ivuliwe.

IZILUMKISO NEZILUMKISO
.Ikhithi kufuneka ivalwe kwaye ikhuselwe kumswakama.
Zonke iisampulu ezilungileyo maziqinisekiswe ngezinye iindlela.
Zonke iisampulu maziphathwe njengezingcolisi ezinokubakho.
.MUSA ukusebenzisa i-reagent ephelelwe lixesha.
.MUSA ukutshintshanisa ii-reagents phakathi kweekiti ezinenani elahlukileyo..
.MUSA ukusebenzisa kwakhona amakhadi ovavanyo kunye nazo naziphi na izixhobo ezilahlwayo.
.Ukusetyenziswa gwenxa, isampuli egqithisileyo okanye encinci kunokubangela ukuphambuka kweziphumo.

LUKULINGANISA
.Njengakweyiphi na i-assay esebenzisa ii-antibodies zegundane, kunokwenzeka ukuba kubekho ukuphazamiseka kwee-antibodies ze-anti-mouse (HAMA) kwi-sampuli. Iisampuli ezivela kwizigulana ezifumene amalungiselelo e-monoclonal antibodies ukuze zixilongwe okanye zonyango zinokuba ne-HAMA. Ezi sampuli zinokubangela iziphumo ezilungileyo okanye ezimbi.
.Esi siphumo sovavanyo sesokubhekisela kwiklinikhi kuphela, akufuneki sisebenze njengesiseko sodwa sokuxilongwa kweklinikhi kunye nonyango, ulawulo lweklinikhi lwezigulane kufuneka luqwalaselwe ngokupheleleyo kunye neempawu zalo, imbali yezonyango, olunye uvavanyo lwelabhoratri, impendulo yonyango, i-epidemiology kunye nolunye ulwazi.
.Le reagent isetyenziswa kuphela kuvavanyo lwe-serum kunye ne-plasma. Isenokungafumani ziphumo zichanekileyo xa isetyenziswa kwezinye iisampuli ezifana namathe nomchamo njl.

IIMPAWU ZOKUSEBENZA

RIINGXELO

1.Hansen JH, et al. Ukuphazamiseka kwe-HAMA kwi-Murine Monoclonal Antibody-Based Immunoassays[J].J ye-Clin Immunoassay,1993,16:294-299.
2.Levinson SS. Uhlobo lwee-Antibodies ze-Heterophilic kunye nendima ekuphazamisekeni kwe-Immunoassay[J].J ye-Clin Immunoassay,1992,15:108-114.

Isitshixo seempawu ezisetyenzisiweyo:

	Isixhobo Sonyango Sokuxilonga se-In Vitro
	Umenzi
	Gcina kwi-2-30℃
	Umhla WOKUPHELELWA
	Musa Ukuphinda Usebenzise
	ISILUMKISO
	Jonga Imiyalelo Yokusetyenziswa

IXiamen Wiz Biotech CO.,LTD
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