UMGAQO

I-membrane yesixhobo sovavanyo igqunywe yi-antigen yeRotavirus Group A kwindawo yovavanyo kunye ne-antibody yebhokhwe yokulwa nomvundla IgG kwindawo yokulawula. I-Lable pad igqunywe yi-fluorescence ebhalwe yi-anti Rotavirus Group A kunye ne-rabbit IgG kwangaphambili. Xa kuvavanywa isampuli echanekileyo, i-RV kwisampuli idibana ne-fluorescence ebhalwe yi-anti Rotavirus Group A, kwaye yenze umxube wokuzikhusela. Phantsi kwesenzo se-immunochromatography, i-complex flow kwicala lephepha elifunxayo. Xa i-complex idlule kwindawo yovavanyo, idibene ne-anti-Rotavirus Group A coating antibody, yenza i-complex entsha. Ukuba i-negative, akukho antigen yeRotavirus Group A kwisampuli, ukuze i-immune complexes ingakwazi ukwakheka, akuyi kubakho mgca obomvu kwindawo yokufumanisa (T). Nokuba i-rotavirus yeqela A ikhona na kwisampuli, i-IgG yegundane ebhalwe yi-latex ifakwa kwi-chromatograph kwindawo yokulawula umgangatho (C) kwaye ibanjwe yi-antibody yebhokhwe yokulwa ne-mouse IgG. Umgca obomvu uya kuvela kwindawo yokulawula umgangatho (C). Umgca obomvu ngumgangatho obonakala kwindawo yokulawula umgangatho (C) wokugweba ukuba kukho iisampulu ezaneleyo na kunye nokuba inkqubo ye-chromatography iqhelekile na. Ikwasetyenziswa njengomgangatho wolawulo lwangaphakathi lwee-reagents.

Inkqubo yoVavanyo:

1. Izigulane ezineempawu kufuneka ziqokelelwe. Ngokweengxelo, ukukhutshwa okuphezulu kwe-rotavirus kwindle yezigulana ezine-gastroenteritis kwenzeka kwiintsuku ezi-3-5 emva kokuqala kwesifo kunye neentsuku ezi-3-13 emva kokuqala kweempawu. Ukuba isampuli iqokelelwe ixesha elide emva korhudo, inani lee-antigens lisenokunganeli ukwenza impendulo entle.

2. Iisampulu maziqokelelwe kwisikhongozeli esicocekileyo, esomileyo, esingangeni manzi esingenazo izicoci kunye nezithinteli.

3. Kwizigulane ezingenaso isifo sohudo, iisampulu zendle eziqokelelweyo akufuneki zibe ngaphantsi kwe-1-2 grams. Kwizigulane ezinesifo sohudo, ukuba indle ilulwelo, nceda uqokelele ubuncinane i-1-2 ml yendle. Ukuba indle inegazi elininzi kunye ne-mucus, nceda uqokelele isampuli kwakhona.

4.Kucetyiswa ukuba kuvavanywe iisampulu kwangoko emva kokuba ziqokelelwe, kungenjalo mazithunyelwe kwilabhoratri zingadlulanga iiyure ezi-6 kwaye zigcinwe kwi-2-8°C. Ukuba iisampulu azikavavanywa zingadlulanga iiyure ezingama-72, mazigcinwe kubushushu obungaphantsi kwe-15°C.

5. Sebenzisa ilindle elitsha ukuvavanya, kwaye usebenzise iisampulu zelindle ezixutywe namanzi anyibilikisiweyo okanye acociweyo