ISIMISO

Ulwelwesi lwedivayisi yokuhlola lumbozwe nge-antibody ye-Tf esifundeni sokuhlola kanye ne-antibody yembuzi yokulwa nonogwaja IgG esifundeni sokulawula. Iphedi yelebula imbozwe nge-antibody ye-antiTf ebhalwe nge-fluorescence kanye ne-antibody ye-IgG enogwaja kusengaphambili. Lapho kuhlolwa isampula enhle, i-antigen ye-Tf esibonelweni ihlangana ne-antibody ye-antiTf ebhalwe nge-fluorescence, bese yakha ingxube yokuzivikela. Ngaphansi kwesenzo se-immunochromatography, ukugeleza kwe-complex kuya ohlangothini lwephepha elimuncayo. Lapho i-complex idlula esifundeni sokuhlola, ihlanganiswe ne-antibody ye-antiTf coating, yakha i-complex entsha.

Uma i-negative, isampula ayinayo i-transferrin noma okuqukethwe kuphansi, ngakho-ke i-immune complex ayikwazi ukwakheka. Ngeke kube khona umugqa obomvu endaweni yokuthola (T). Kungakhathaliseki ukuthi i-Tf antibody ikhona yini kusampula noma cha, i-antibody yegundane elimele i-colloidal gold-labeled mouse anti-human IgM monoclonal kanye ne-antibody ye-goat anti-mouse IgG embozwe emgqeni wendawo yokulawula ikhwalithi (C). Ngemuva kwalokho ama-agglutinates athuthukisa umbala endaweni yokulawula ikhwalithi, futhi umugqa obomvu uzovela ku-(C). Umugqa obomvu uyindinganiso ebonakala endaweni yokulawula ikhwalithi (C) yokunquma ukuthi kukhona amasampula anele nokuthi inqubo ye-chromatography ijwayelekile yini. Isetshenziswa futhi njengendinganiso yokulawula yangaphakathi yama-reagents.

Inqubo Yokuhlola

Sicela ufunde isethulo sephakheji ngaphambi kokuhlola.

1. Khipha ikhadi lokuhlola esikhwameni se-foil, ulibeke etafuleni elilinganayo bese ulimaka.

2. Susa isivalo epayipini lesampula bese ulahla amaconsi amabili okuqala esampula exutshwe, engeza amaconsi amathathu (cishe ama-uL ayi-100) isampula exutshwe nebhamuza eqonde phezulu futhi kancane kancane emthonjeni wesampula wekhadi nge-dispette enikeziwe. Bese uqala i-timer.