Ikhithi Yokuxilonga ye-25-hydroxy Vitamin D(ukuhlolwa kwe-fluorescence immunochromatographic)
Ukusetshenziswa kokuxilonga ngaphakathi kwesibeletho kuphela
Sicela ufunde lesi sithasiselo sephakheji ngokucophelela ngaphambi kokusebenzisa futhi ulandele imiyalelo ngokucophelela. Ukuthembeka kwemiphumela yokuhlola akunakuqinisekiswa uma kukhona ukuphambuka emiyalweni ekulesi sithasiselo sephakheji.

UKUSETSHENZISWA OKUHLOSIWE
Ikhithi Yokuxilonga ye-25-hydroxy Vitamin D (i-fluorescence immunochromatographic assay) iyi-fluorescence immunochromatographic assay yokuthola inani le-25-hydroxy Vitamin D (25-(OH)VD) ku-serum noma i-plasma yomuntu, esetshenziswa kakhulu ukuhlola amazinga e-vitamin D. Iyi-reagent yokuxilongwa eyisizayo. Zonke izibonelo ezinhle kumele ziqinisekiswe ngezinye izindlela. Lokhu kuhlolwa kuhloselwe ukusetshenziswa kochwepheshe bezempilo kuphela.

ISIFINYEZO
I-Vitamin D iyi-vitamin futhi iyi-hormone ye-steroid, ikakhulukazi ehlanganisa i-VD2 ne-VD3, ukwakheka kwayo kufana kakhulu. I-Vitamin D3 ne-D2 ziguqulwa zibe yi-25 hydroxyl vitamin D (kufaka phakathi i-25-dihydroxyl vitamin D3 ne-D2). I-25-(OH) VD emzimbeni womuntu, ukwakheka okuzinzile, ukuhlushwa okuphezulu. I-25-(OH) VD ibonisa inani eliphelele le-vitamin D, kanye nekhono lokuguqula i-vitamin D, ngakho-ke i-25-(OH)VD ibhekwa njengesibonakaliso esihle kakhulu sokuhlola izinga le-vitamin D. I-Diagnostic Kit isekelwe ku-immunochromatography futhi inganikeza umphumela zingakapheli imizuzu eyi-15.

ISIMISO SENQUBO
I-membrane yedivayisi yokuhlola imbozwe nge-conjugate ye-BSA kanye ne-25-(OH)VD esifundeni sokuhlola kanye ne-antibody yembuzi yokulwa nonogwaja IgG esifundeni sokulawula. Iphedi yomaka imbozwe nge-antibody ye-fluorescence mark anti 25-(OH)VD kanye ne-IgG yonogwaja kusengaphambili. Lapho kuhlolwa isampula, i-25-(OH)VD kusampula ihlangana ne-antibody ye-antibody ye-fluorescence mark anti 25-(OH)VD, bese yakha ingxube yokuzivikela komzimba. Ngaphansi kwesenzo se-immunochromatography, ukugeleza okuyinkimbinkimbi kuya ohlangothini lwephepha elimuncayo, lapho i-complex idlula esifundeni sokuhlola, i-free fluorescent marker izohlanganiswa ne-25-(OH)VD ku-membrane. Ukuhlushwa kwe-25-(OH)VD kuwukuxhumana okungekuhle kwesignali ye-fluorescence, futhi ukuhlushwa kwe-25-(OH)VD kusampula kungatholakala nge-fluorescence immunoassay assay.

IZINTO EZINGENAYO NEZINTO EZINIKEZWE

Izingxenye zephakheji ezingama-25T：
Ikhadi lokuhlola ngalinye lifakwe ifoyili ngesikhwama se-desiccant esingu-25T
Isixazululo .I-25T
Isixazululo se-.B 1
.Iphakheji yokufaka 1

KUDINGEKA IZINTO EZISETSHENZISWAYO KODWA AZINIKEZWA
Isitsha sokuqoqa isampula, isibali sikhathi

IQOQO LEZIBONELO NOKUGCINA
1. Amasampula ahlolwe angaba yi-serum, i-heparin anticoagulant plasma noma i-EDTA anticoagulant plasma.
2. Ngokwezindlela ezijwayelekile zokuqoqa isampula. Isampula ye-serum noma ye-plasma ingagcinwa efrijini ku-2-8℃ izinsuku eziyi-7 kanye nokugcinwa ngaphansi kuka-15°C izinyanga eziyi-6.
3. Zonke izibonelo zigwema imijikelezo yokuqandisa nokuncibilikisa.

INQUBO YOKUHLOLA
Inqubo yokuhlola yale thuluzi bheka incwadi ye-immunoanalyzer. Inqubo yokuhlola i-reagent imi kanje
1. Beka eceleni wonke ama-reagent namasampula ukuze izinga lokushisa legumbi lifinyelele ezingeni lokushisa legumbi.
2.Vula i-Portable Immune Analyzer (WIZ-A101), faka ukungena ngemvume kwephasiwedi ye-akhawunti ngokwendlela yokusebenza kwethuluzi, bese ufaka isikhombikubona sokuthola.
3.Skena ikhodi yokuqinisekisa ukuze uqinisekise into yokuhlola.
4. Khipha ikhadi lokuhlola esikhwameni se-foil.
5.Faka ikhadi lokuhlola embobeni yekhadi, skena ikhodi ye-QR, bese unquma into yokuhlolwa.
6. Faka isampula ye-serum noma i-plasma engu-30μL esixazululweni se-A, bese uxuba kahle.
7. Faka isisombululo se-50μL B engxubeni engenhla, bese uxuba kahle.
8.Shiya ingxube imizuzu eyi-15.
9. Faka ingxube engu-80μL ukuze uhlole umthombo wekhadi.
10. Chofoza inkinobho ethi "ukuhlolwa okujwayelekile", ngemva kwemizuzu eyi-10, ithuluzi lizobona ngokuzenzakalelayo ikhadi lokuhlola, lingafunda imiphumela esikrinini sokubonisa sethuluzi, bese liqopha/liphrinta imiphumela yokuhlolwa.
11. Bheka imiyalelo ye-Portable Immune Analyzer (WIZ-A101).

AMANANI ALINDELEKILE
25-(OH)VD ububanzi obujwayelekile: 30-100ng/mL
Kunconywa ukuthi ilabhorethri ngayinye isungule ububanzi bayo obujwayelekile obumele iziguli zayo.

IMIPHUMELA YOKUHLOLA NOKUHLUKANISWA
.Idatha engenhla iyisikhathi sokubhekisela esimiselwe idatha yokuthola yale khithi, futhi kuphakanyiswa ukuthi ilabhorethri ngayinye kufanele isungule isikhathi sokubhekisela sokubaluleka komtholampilo okufanelekile kwabantu kulesi sifunda.
.Ukuhlushwa kwe-25-(OH)VD kuphakeme kunobubanzi bokubhekisela, futhi izinguquko zomzimba noma impendulo yokucindezeleka kufanele ikhishwe. Ngempela okungavamile, kufanele kuhlanganiswe ukuxilongwa kwezimpawu zomtholampilo.
.Imiphumela yale ndlela isebenza kuphela kububanzi bokubhekisela obusungulwe yile ndlela, futhi imiphumela ayifani ngqo nezinye izindlela.
Ezinye izici zingadala amaphutha emiphumeleni yokuthola, okuhlanganisa izizathu zobuchwepheshe, amaphutha okusebenza kanye nezinye izici zesampula.

ISITOKELO NOKUZINZIMA
.Ikhithi ihlala isikhathi eside kusukela ngosuku lokwenziwa kwayo. Gcina amakhithi angasetshenziswanga ku-2-30°C. UNGAWAQANDISI. Ungayisebenzisi ngemva kosuku lokuphelelwa yisikhathi.
.Ungavuli isikhwama esivaliwe uze ulungele ukwenza isivivinyo, futhi isivivinyo sokusetshenziswa kanye sinconywa ukuthi sisetshenziswe ngaphansi kwendawo edingekayo (izinga lokushisa 2-35℃, umswakama 40-90%) zingakapheli imizuzu engama-60 ngokushesha okukhulu.
.Isampula yokuxuba isetshenziswa ngokushesha ngemva kokuvulwa.

IZIXWAYISO NEZIQAPHELO ZOKWENZA
.Ikhithi kufanele ivalwe futhi ivikelwe emswakama.
Zonke izibonelo ezinhle kufanele ziqinisekiswe ngezinye izindlela.
Zonke izibonelo kufanele ziphathwe njengezingcolisi ezingaba khona.
UNGASEBENZISI i-reagent ephelelwe yisikhathi.
UNGASHINTSHI ama-reagent phakathi kwamakhithi anenombolo ehlukene yezindawo..
UNGASEBENZISI kabusha amakhadi okuhlola nanoma yiziphi izesekeli ezilahlwayo.
.Ukusebenza kabi, isampula eningi kakhulu noma encane kungaholela ekuphambukeni kwemiphumela.

LUKULINGISA
.Njenganoma yikuphi ukuhlolwa okusebenzisa ama-antibodies egundane, kungenzeka ukuthi kukhona ukuphazamiseka kwama-antibodies e-anti-mouse (HAMA) esampula. Amasampula avela ezigulini ezithole amalungiselelo ama-antibodies e-monoclonal ukuze kutholakale isifo noma ukwelashwa angase abe ne-HAMA. Amasampula anjalo angabangela imiphumela emibi noma emibi engamanga.
.Lo mphumela wokuhlolwa ungowokubhekisela emtholampilo kuphela, akufanele usebenze njengesisekelo sokuxilongwa nokwelashwa kwezokwelapha kuphela, ukuphathwa kwezokwelapha kweziguli kufanele kucatshangelwe ngokuphelele kuhlanganiswe nezimpawu zaso, umlando wezokwelapha, okunye ukuhlolwa kwelabhorethri, impendulo yokwelashwa, i-epidemiology kanye nolunye ulwazi.
.Le reagent isetshenziswa kuphela ekuhlolweni kwe-serum kanye ne-plasma. Ingase ingatholi umphumela onembile uma isetshenziswa kwamanye amasampula afana namathe nomchamo njll.

IZIMALI ZOKUSEBENZA

RIZINKOMBA

1.Hansen JH, nabanye.Ukuphazamiseka kwe-HAMA nge-Murine Monoclonal Antibody-Based Immunoassays[J].J ye-Clin Immunoassay,1993,16:294-299.
2.Levinson SS. Uhlobo Lwama-Antibodies Angama-Heterophilic kanye Nendima Ekuphazamisekeni Kwe-Immunoassay[J].J we-Clin Immunoassay,1992,15:108-114.

Isihluthulelo sezimpawu ezisetshenzisiwe:

	Idivayisi Yezokwelapha Yokuxilonga E-In Vitro
	Umkhiqizi
	Gcina ku-2-30℃
	Usuku lokuphelelwa isikhathi
	Ungasebenzisi Kabusha
	ISEXWAYISO
	Bheka Imiyalelo Yokusebenzisa

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