ISIMISO

I-membrane yedivayisi yokuhlola imbozwe nge-antigen ye-Rotavirus Group A esifundeni sokuhlola kanye ne-antibody ye-IgG yembuzi elwa nonogwaja esifundeni sokulawula. I-lable pad imbozwe nge-fluorescence ebhalwe nge-anti Rotavirus Group A kanye ne-IgG yenogwaja kusengaphambili. Lapho kuhlolwa isampula enhle, i-RV esesampula ihlangana ne-anti Rotavirus Group A ebhalwe nge-fluorescence, bese yakha ingxube ye-immune. Ngaphansi kwesenzo se-immunochromatography, ukugeleza kwe-complex kuya ohlangothini lwephepha elimuncayo. Lapho i-complex idlula esifundeni sokuhlola, ihlanganiswe ne-antibody ye-anti-Rotavirus Group A, yakha i-complex entsha. Uma i-negative, ayikho i-antigen ye-Rotavirus Group A esibonelweni, ukuze ama-immune complex angakhiwa, ngeke kube khona umugqa obomvu endaweni yokuthola (T). Kungakhathaliseki ukuthi i-rotavirus yeqembu A ikhona yini esibonelweni, i-IgG yegundane ene-latex ifakwa i-chromatograph endaweni yokulawula ikhwalithi (C) futhi ibanjwe yi-antibody ye-IgG yegundane elwa nonogwaja. Umugqa obomvu uzovela endaweni yokulawula ikhwalithi (C). Umugqa obomvu uwukuthi indinganiso ivela endaweni yokulawula ikhwalithi (C) yokunquma ukuthi ngabe kukhona amasampula anele nokuthi inqubo ye-chromatography ijwayelekile yini. Iphinde isetshenziswe njengendinganiso yokulawula yangaphakathi yama-reagent.

Inqubo Yokuhlola:

1. Iziguli ezinezimpawu kufanele ziqoqwe. Ngokusho kwemibiko, ukukhishwa okuphezulu kwe-rotavirus endle yeziguli ezine-gastroenteritis kwenzeka ezinsukwini ezingu-3-5 ngemuva kokuqala kwesifo kanye nezinsuku ezingu-3-13 ngemuva kokuqala kwezimpawu. Uma isampula iqoqwa isikhathi eside ngemva kohudo, inani lama-antigen lingase linganeli ukwenza ukusabela okuhle.

2. Amasampula kufanele aqoqwe esitsheni esihlanzekile, esomile, esingangeni manzi esingenazo izihlanzi kanye nezilondolozi.

3. Ezigulini ezingenaso isifo sohudo, amasampula endle eqoqwe akufanele abe ngaphansi kwamagremu angu-1-2. Ezigulini ezinesifo sohudo, uma indle iwuketshezi, sicela uqoqe okungenani u-1-2 ml wendle. Uma indle iqukethe igazi eliningi kanye ne-mucus, sicela uqoqe isampula futhi.

4. Kunconywa ukuhlola amasampula ngokushesha ngemva kokuqoqwa, ngaphandle kwalokho kufanele athunyelwe elabhorethri zingakapheli amahora ayi-6 futhi agcinwe ku-2-8°C. Uma amasampula engakahlolwa zingakapheli amahora angama-72, kufanele agcinwe ezingeni lokushisa elingaphansi kuka-15°C.

5. Sebenzisa indle entsha ukuhlola, kanye namasampula endle axutshwe namanzi acwengekile noma acwengekile