UMGAQO

I-membrane yesixhobo sovavanyo igqunywe nge-antibody ye-Tf kwindawo yovavanyo kunye ne-antibody ye-IgG yebhokhwe kwindawo yokulawula. Ii-lable pad zigqunywe nge-antibody ye-antibody ye-Tf ebhalwe nge-fluorescence kunye ne-antibody ye-IgG yerabbit kwangaphambili. Xa kuvavanywa isampuli elungileyo, i-antigen ye-Tf kwisampuli idibana ne-antibody ye-antibody ye-fluorescence ebhalwe nge-antiTf, kwaye yenze umxube womzimba. Phantsi kwesenzo se-immunochromatography, ukuhamba kwe-complex kwicala lephepha elifunxayo. Xa i-complex idlule kwindawo yovavanyo, idibene ne-antibody ye-antiTf coating, yenza i-complex entsha.

Ukuba ayibonakali, isampuli ayinayo i-transferrin okanye umxholo uphantsi, ukuze i-immune complex ingakwazi ukwakheka. Akuyi kubakho mgca ubomvu kwindawo yokubona (T). Nokuba i-Tf antibody ikhona kwisampuli okanye ayikho, i-antibody eseleyo ye-colloidal gold-labeled mouse anti-human IgM monoclonal kunye ne-antibody ye-goat anti-mouse IgG zigqunywe kumgca wendawo yokulawula umgangatho (C). Emva koko ii-agglutinates zivelisa umbala kwindawo yokulawula umgangatho, kwaye umgca obomvu uya kuvela ku-(C). Umgca obomvu ngumgangatho obonakala kwindawo yokulawula umgangatho (C) wokugweba ukuba kukho iisampulu ezaneleyo kunye nokuba inkqubo ye-chromatography iqhelekile na. Ikwasetyenziswa njengomgangatho wolawulo lwangaphakathi lwee-reagents.

Inkqubo yoVavanyo

Nceda ufunde iphakheji engezantsi ngaphambi kokuba uyivavanye.

1.Khupha ikhadi lovavanyo kwingxowa yefoyile, ulibeke etafileni elinganayo uze uliphawule.

2. Susa isiciko kwityhubhu yesampulu uze ulahle amathontsi amabini okuqala esampuli exutyiweyo, yongeza amathontsi amathathu (malunga ne-100uL) isampuli exutyiweyo engenamaqamza ngokuthe nkqo kwaye kancinci kancinci kwiqula lesampulu yekhadi ene-dispette enikiweyo. Emva koko qala i-timer.