ỤKPỤRỤ

A na-ekpuchi akpụkpọ ahụ nke ngwaọrụ nnwale ahụ na mgbochi EV71 na mpaghara nnwale ahụ na mgbochi EV71 nke ewu na-alụso oke bekee na mpaghara njikwa. A na-ekpuchi mpempe akwụkwọ label ahụ site na iji ọgwụ mgbochi EV71 nke a na-akpọ anti EV71 na oke bekee IgG tupu oge eruo. Mgbe a na-anwale ihe nlele dị mma, antigen EV71 dị na ihe nlele ahụ na-ejikọta ya na ọgwụ mgbochi EV71 nke a na-akpọ fluorescence, ma na-emepụta ngwakọta mgbochi. N'okpuru ọrụ nke chromatography, usoro mgbagwoju anya na-aga n'ihu nke akwụkwọ na-amịkọrọ mmiri, mgbe mgbagwoju anya gafere mpaghara nnwale ahụ, ọ jikọtara ya na mgbochi mgbochi EV71 nke a na-akpọ antibody mkpuchi, na-emepụta mgbagwoju anya ọhụrụ.

Ọ bụrụ na ọ dị njọ, ihe nlele ahụ enweghị ihe mgbochi enterovirus 71 IgM, nke mere na enweghị ike ịmepụta ihe mgbochi ahụ ji alụso ọrịa ọgụ. Agaghị enwe ahịrị uhie na mpaghara nchọpụta (T). N'agbanyeghị ma ọgwụ mgbochi Enterovirus 71 IgM dị na ihe nlele ahụ ma ọ bụ na ọ dị, ọgwụ mgbochi monoclonal nke colloidal ọlaedo nke e ji akara ọla edo mee na ihe mgbochi IgM monoclonal nke òké na-emegide òké ewu nke a kpụchara n'ebe njikwa mma (C) na-ejikọ. Mgbe ahụ, agglutinates na-etolite agba na mpaghara njikwa mma, ahịrị uhie ga-apụtakwa na (C). Ahịrị uhie bụ ọkọlọtọ ahụ na-apụta na mpaghara njikwa mma (C) iji chọpụta ma enwere ihe nlele zuru oke na ma usoro chromatography ọ bụ ihe nkịtị. A na-ejikwa ya dị ka ọkọlọtọ njikwa dị n'ime maka ihe ndị na-eme ihe.

Usoro Ule:

1. Ihe nlele ndị a nwalere nwere ike ịbụ ọbara zuru oke, gụnyere ọbara akwara ma ọ bụ ọbara dị n'akụkụ. A gaghị echekwa ọbara dum mgbe a chịkọtara ya. Ekwesịrị iji ya ozugbo m chịkọtara ya.

2. A na-anakọta ihe nlele ọbara site na iji usoro ọkọlọtọ. Enweghị ike iji serum nke anaghị arụ ọrụ n'ọkụ. A naghị atụ aro ka e jiri serum lipemic, turbid ma ọ bụ nke ruru unyi. Ihe dị n'ime serum. Mmiri ozuzo ga-emetụta nsonaazụ nnwale ahụ, a ga-etinye ihe nlele ndị dị otú ahụ n'ime centrifuge ma ọ bụ nzacha tupu e jiri ya.

3. Ihe nlele ndị a nwalere nwere ike ịbụ heparin, sodium citrate ma ọ bụ EDTA anticoagulant plasma.

4. Dịka usoro ọkọlọtọ si dị, a pụrụ idobe ihe nlele ọbara ma ọ bụ plasma n'ime friji na okpomọkụ 2-8℃ ruo ụbọchị atọ, a pụkwara idobe ihe nlele ahụ n'okpuru -15°C ruo ọnwa atọ.

5. Ihe nlele niile zere usoro oyi kpọnwụrụ akpọnwụ.