Kete Tātaritanga mō te Huaora D 25-hydroxy(whakamātautau ārai-ira-whakaahua-whakaahua)
Mō te whakamahinga tātaritanga in vitro anake
Pānuihia tēnei tāpiringa i mua i te whakamahinga, ā, me whai pūmau i ngā tohutohu. Kāore e taea te whakamana i te pono o ngā hua whakamātautau mēnā he rerekētanga mai i ngā tohutohu kei roto i tēnei tāpiringa.

TE WHAKAMAHINGA I WHAKATAURANGIA
Ko te Kete Tātaritanga mō te Huaora D 25-hydroxy (whakamātautau immunochromatographic fluorescence) he whakamātautau immunochromatographic fluorescence mō te kimi i te nui o te Huaora D 25-hydroxy (25-(OH)VD) i roto i te toto tangata, i te plasma rānei, e whakamahia ana hei aromatawai i ngā taumata o te huaora D. He matū tātaritanga āwhina. Me whakaū ngā tauira pai katoa mā ētahi atu tikanga. Ko tēnei whakamātautau he mea hanga mō te whakamahinga hauora anake.

WHAKARĀPOPOTONGA
He huaora te Huaora D, ā, he homoni steroid anō hoki, ko te nuinga kei roto ko te VD2 me te VD3, ā, he tino ōrite te hanganga. Ka hurihia te Huaora D3 me te D2 ki te 25 hydroxyl huaora D (tae atu ki te 25-dihydroxyl huaora D3 me te D2). He 25-(OH) VD i roto i te tinana tangata, he hanganga pumau, he kukū teitei. Ko te 25-(OH) VD e whakaata ana i te nui katoa o te huaora D, me te kaha hurihanga o te huaora D, nō reira e kiia ana ko te 25-(OH) VD te tohu pai rawa atu mō te aromatawai i te taumata o te huaora D. Ko te Kete Tātaritanga e hangai ana ki te immunochromatography, ā, ka taea te whakaputa hua i roto i te 15 meneti.

TE MĀTĀPONO O TE TIKANGA
Kua pania te kiriuhi o te taputapu whakamātautau ki te hononga o te BSA me te 25-(OH)VD i te rohe whakamātautau me te paturopi IgG ārai rāpeti koati i te rohe whakahaere. Kua pania ngā papa tohu ki te tohu fluorescence ārai rāpeti 25-(OH)VD me te IgG rāpeti i mua. I te whakamātautau i te tauira, ka honoa te 25-(OH)VD i roto i te tauira ki te paturopi ārai rāpeti kua tohua ki te fluorescence, ā, ka hanga he ranunga ārai mate. I raro i te mahi a te immunochromatography, ka rere te matatini i te ahunga o te pepa mimiti, ina paahitia e te matatini te rohe whakamātautau, ka honoa te tohu fluorescence kore utu ki te 25-(OH)VD i runga i te kiriuhi. Ko te kukū o te 25-(OH)VD he hononga kino mō te tohu fluorescence, ā, ka taea te kite i te kukū o te 25-(OH)VD i roto i te tauira mā te whakamātautau immunoassay fluorescence.

NGĀ RĀWENI ME NGĀ RAUEMI I TUKUNA

Ngā wāhanga mōkihi 25T：
Kāri whakamātautau takitahi he pepa konutea kua kapi ki te whakamaroke 25T
.He otinga 25T
Otinga .B 1
.Whakaurunga mōkihi 1

NGĀ RAUEMI E HIAHIATIA ANA ENGARI KĀORE I HOATU
Ipu kohinga tauira, taima

TE KOHINGA TAUIRA ME TE ROKIROKI
1. Ko ngā tauira i whakamatautauria he toto toto, he toto toto heparin anticoagulant, he toto toto EDTA anticoagulant rānei.
2. E ai ki ngā tikanga paerewa, kohia he tauira. Ka taea te pupuri i te tauira toto, i te tauira plasma rānei ki roto i te pouaka whakamātao i te 2-8℃ mō ngā rā e 7, ā, ka taea te pupuri i te tauira i raro i te -15°C mō ngā marama e 6.
3. Kaua ngā tauira katoa e huri i te huka me te rewa.

TE TIKANGA WHAKAMĀTAUTAU
Tirohia te pukapuka ā-ringa mō te whakamātautau o te taputapu. Ko te tukanga whakamātautau matū koia tēnei:
1. Waiho ngā matū me ngā tauira katoa ki te pāmahana rūma.
2. Whakatuwheratia te Pūnaha Tātari Ārai Mate Kawe (WIZ-A101), tāuruhia te kupuhipa takiuru pūkete e ai ki te tikanga whakahaere o te taputapu, ka tomo ki te atanga kimi.
3. Matawaihia te waehere whakaū niho hei whakaū i te tūemi whakamātautau.
4. Tangohia te kāri whakamātautau i te putea pepa konutea.
5. Whakauruhia te kāri whakamātautau ki roto i te mokamoka kāri, matawaihia te waehere QR, ka kimihia te tūemi whakamātautau.
6. Tāpirihia he 30μL o te tauira toto, o te tauira parāuma rānei ki te otinga A, ka whakaohokia kia pai.
7. Tāpirihia te 50μL o te otinga B ki te ranunga i runga ake nei, ka whakaohokia kia pai.
8.Waiho te ranunga mō te 15 meneti.
9. Tāpirihia he ranunga 80μL ki te puna tauira o te kāri.
10. Pāwhiritia te pātene "whakamātautau paerewa", i muri i te 10 meneti, ka kitea aunoa e te taputapu te kāri whakamātautau, ka taea te pānui i ngā hua mai i te mata whakaatu o te taputapu, ka tuhi/tā i ngā hua whakamātautau.
11. Tirohia ngā tohutohu mō te Pūnaha Tātari Ārai Mate Kawe (WIZ-A101).

NGĀ UARA E TŪMANAKOHIA ANA
Awhe noa 25-(OH)VD: 30-100ng/mL
E taunakihia ana kia whakatūhia e ia taiwhanga tōna ake awhe noa e tohu ana i te taupori tūroro.

NGĀ HUA WHAKAMĀTAUTAU ME TE WHAKAMĀRAMATANGA
Ko ngā raraunga o runga ake nei te wā tohutoro kua whakatūria mō ngā raraunga kitenga o tēnei kete, ā, e kīia ana me whakatū e ia taiwhanga he wā tohutoro mō te hiranga haumanu e tika ana mō te taupori i tēnei rohe.
.He teitei ake te kukū o te 25-(OH)VD i te awhe tohutoro, ā, me tango atu ngā huringa ā-tinana, te urupare ahotea rānei. He tino rerekē, me whakakotahi te tātaritanga tohu haumanu.
Ko ngā hua o tēnei tikanga ka pā anake ki te awhe tohutoro kua whakatūria e tēnei tikanga, ā, kāore ngā hua e taea te whakarite tika ki ētahi atu tikanga.
Ka taea hoki e ētahi atu āhuatanga te whakaputa hapa i ngā hua kimi, tae atu ki ngā take hangarau, ngā hapa whakahaere me ētahi atu āhuatanga tauira.

TE ROKIROKI ME TE PŪMAU
.E 18 marama te roa o te oranga o te kete mai i te rā i hangaia ai. Penapenahia ngā kete kāore anō kia whakamahia ki te 2-30°C. KAUA E WHAKATIO. Kaua e whakamahia i tua atu i te rā paunga.
Kaua e huakina te putea hiri kia rite rā anō koe ki te mahi i tētahi whakamātautau, ā, e taunakihia ana kia whakamahia te whakamātautau kotahi-whakamahinga i raro i te taiao e hiahiatia ana (pāmahana 2-35℃, haumākū 40-90%) i roto i te 60 meneti i te wā tere rawa atu.
Ka whakamahia te wai whakawera tauira i muri tonu i te whakatuwheratanga.

NGĀ WHAKATŪPATO ME NGĀ WHAKATŪPATO
Me hiri te kete, ā, me tiaki hoki i te makuku.
Me whakamana ngā tauira pai katoa mā ētahi atu tikanga.
Me whakaaro ngā tauira katoa he poke pea.
KAUA e whakamahia te matū kua pau te wā.
.KAUA e whakawhiti i ngā matū i waenga i ngā kete he rerekē te nama rota..
KAUA e whakamahi anō i ngā kāri whakamātautau me ngā taputapu whakakorekore.
.Mēnā he te whakamahinga, he nui rawa, he iti rānei te tauira, ka rerekē pea ngā hua.

LWHAKATAURANGA
Pērā i ngā whakamātautau katoa e whakamahi ana i ngā paturopi kiore, tera pea ka pāngia te tauira e ngā paturopi ārai-kiore tangata (HAMA). Tērā pea kei roto i ngā tauira mai i ngā tūroro kua whiwhi i ngā whakaritenga o ngā paturopi monoclonal mō te tātaritanga, te rongoā rānei, te HAMA. Tērā pea ka puta he hua hē, he hua hē rānei.
Hei tohutoro haumanu anake tēnei hua whakamātautau, kaua e waiho hei pūtake anake mō te tātaritanga haumanu me te maimoatanga, me whai whakaaro whānui te whakahaere haumanu o ngā tūroro me ōna tohu, hītori hauora, ētahi atu whakamātautau taiwhanga, urupare maimoatanga, mate urutā me ētahi atu mōhiohio.
Ka whakamahia anake tēnei matū hei whakamātautau i te toto me te toto toto. Tērā pea kāore e tika te hua ina whakamahia mō ētahi atu tauira pēnei i te huware me te mimi me ētahi atu.

NGĀ ĀHUATANGA MAHI

RNGĀ WHAKAMĀRAMA

1.Hansen JH, et al. Te Whakararuraru HAMA ki ngā Immunoassays Murine Monoclonal Antibody-Based Immunoassays[J].J o Clin Immunoassay,1993,16:294-299.
2.Levinson SS. Te Āhua o ngā Paturopi Heterophilic me te Tūranga i roto i te Whakararuraru Immunoassay[J].J o Clin Immunoassay,1992,15:108-114.

Ngā tohu matua i whakamahia:

	Pūrere Hauora Tātaritanga In Vitro
	Kaihanga
	Penapena ki te 2-30℃
	Rā Paunga
	Kaua e Whakamahi Anō
	WHAKATŪPATO
	Tirohia ngā Tohutohu Whakamahinga

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