MFUNDO

Chipolopolo cha chipangizo choyesera chimakutidwa ndi antibody ya EV71 pamalo oyesera ndi antibody ya mbuzi ya IgG pamalo olamulira. Mapepala olembera amakutidwa ndi antibody ya EV71 yolembedwa kuti fluorescence ndi antibody ya kalulu IgG pasadakhale. Poyesa chitsanzo chabwino, antigen ya EV71 mu chitsanzo imaphatikizana ndi antibody ya EV71 yolembedwa kuti fluorescence, ndikupanga chisakanizo cha chitetezo chamthupi. Pansi pa ntchito ya chromatography, kuyenda kwa zovuta kumayang'ana pepala loyamwa, pamene zovutazo zidadutsa pamalo oyesera, zimaphatikizana ndi antibody ya antiEV71, zimapanga zovuta zatsopano.

Ngati ili ndi kachilombo koyambitsa matenda, chitsanzocho sichili ndi ma antibody a enterovirus 71 IgM, kotero kuti chitetezo cha mthupi sichingapangidwe. Sipadzakhala mzere wofiira m'dera lozindikira (T). Kaya pali ma antibody a Enterovirus 71 IgM mu chitsanzocho kapena ayi, ma antibody otsala a mbewa yotsutsana ndi anthu a IgM monoclonal ndi ma antibody a mbuzi a IgG okhala ndi chizindikiro chagolide omwe ali ndi chizindikiro chagolide omwe ali ndi chizindikiro chagolide komanso ma antibody a mbuzi a IgG okhala ndi chizindikiro chagolide omwe ali ndi chizindikiro cha khalidwe (C) amamangirirana. Kenako ma agglutinates amapanga mtundu m'dera lowongolera khalidwe, ndipo mzere wofiira udzawonekera mu (C). Mzere wofiira ndi muyezo womwe umawonekera m'dera lowongolera khalidwe (C) kuti muwone ngati pali zitsanzo zokwanira komanso ngati njira ya chromatography ndi yachibadwa. Imagwiritsidwanso ntchito ngati muyezo wamkati wa ma reagents.

Njira Yoyesera:

1. Zitsanzo zomwe zayesedwa zitha kukhala magazi athunthu, kuphatikizapo magazi a m'mitsempha kapena magazi a m'mbali. Magazi athunthu sangasungidwe mutatenga. Ndiyenera kugwiritsidwa ntchito nthawi yomweyo mutatenga.

2. Zitsanzo za seramu zimasonkhanitsidwa popanda kuwononga maselo motsatira njira zodziwika bwino. Seramu yosagwiritsidwa ntchito ndi kutentha singagwiritsidwe ntchito. Sikoyenera kugwiritsa ntchito seramu yamafuta, yokhuthala kapena yodetsedwa. Tinthu tating'onoting'ono mu seramu. Ndipo mvula idzakhudza zotsatira za mayeso, zitsanzo zotere ziyenera kuyikidwa mu centrifuge kapena kusefedwa musanagwiritse ntchito.

3. Zitsanzo zomwe zayesedwa zitha kukhala heparin, Sodium citrate kapena EDTA anticoagulant plasma.

4. Kutengera njira zodziwika bwino, sonkhanitsani chitsanzo. Seramu kapena plasma ikhoza kusungidwa mufiriji pa 2-8℃ kwa masiku atatu ndikusungidwa pansi pa -15°C kwa miyezi itatu.

5. Zitsanzo zonse kupewa kuzizira ndi kusungunula madzi.