Agakoresho ko gusuzuma indwara ka Vitamine D 25-hydroxy(isuzuma rya fluorescence immunochromatographic assay)
Gukoreshwa mu gusuzuma indwara mu buryo bwa "in vitro" gusa
Mbere yo gukoresha iyi paki, soma witonze kandi ukurikize amabwiriza neza. Ntabwo bishoboka kwemeza ko ibisubizo by'isuzuma ari ukuri niba hari ibitagenda neza ku mabwiriza ari muri iyi paki.

IKORESHWA RYITEGANYANYE
Ikizamini cyo gusuzuma indwara cya Vitamine D ya 25-hydroxy (fluorescence immunochromatographic assay) ni ikizamini cyo gusuzuma indwara ya Vitamine D ya 25-hydroxy (25-(OH)VD) mu maraso y'umuntu cyangwa mu maraso, gikoreshwa cyane cyane mu gusuzuma urugero rwa Vitamine D. Ni reagent y'inyongera yo gusuzuma indwara. Ikizamini cyose cyagaragaye ko gifite ubwandu kigomba kwemezwa n'ubundi buryo. Iki kizamini kigenewe gukoreshwa n'abaganga gusa.

INSHAMAKE
Vitamine D ni vitamine kandi ni imisemburo ya steroid, cyane cyane irimo VD2 na VD3, imiterere yayo isa cyane. Vitamine D3 na D2 bihindurwamo 25 hydroxyl vitamine D (harimo 25-dihydroxyl vitamine D3 na D2). 25-(OH) VD mu mubiri w'umuntu, imiterere ihamye, ubwinshi bwayo bwinshi. 25-(OH) VD igaragaza ingano yose ya vitamine D, n'ubushobozi bwa vitamine D mu guhindura, bityo 25-(OH)VD ifatwa nk'ikimenyetso cyiza cyo gusuzuma urwego rwa vitamine D. Iyi Diagnostic Kit ishingiye kuri immunochromatography kandi ishobora gutanga igisubizo mu minota 15.

IHAME RY'UBURYO BWAYO
Agace k'igikoresho cyo gupima gatwikiriwe n'agace ka BSA na 25-(OH)VD ku gace k'igeragezwa n'agace ka anti-inkwavu ka IgG ku gace kagenzurwa. Agapapuro k'ikarita gatwikiriwe n'agace ka anti-inkwavu ka 25-(OH)VD kari ku gace kagenzurwa mbere y'igihe. Mu gihe cyo gupima icyitegererezo, 25-(OH)VD mu gipimo ihuzwa n'agace ka anti-inkwavu ka 25-(OH)VD kari ku gace kagenzurwa, maze bigakora uruvange rw'ubudahangarwa bw'umubiri. Mu gihe cy'igikorwa cya immunochromatography, urujya n'uruza rw'uruvange mu cyerekezo cy'impapuro zifata, iyo agace kanyuze mu gace kagenzurwa, ikimenyetso cya fluorescent cyigenga kizahuzwa na 25-(OH)VD ku gace kagenzurwa. Igipimo cya 25-(OH)VD ni isano iri hagati y'ikimenyetso cya fluorescence, kandi igipimo cya 25-(OH)VD mu gipimo gishobora kumenyekana hakoreshejwe isuzuma ry'ubudahangarwa bw'umubiri.

IBIKORESHO N'IBIKORESHO BYATANZWE

Ibice by'ipaki bya 25T:
Ikarita yo gupima ku giti cyayo ipfunyitse mu gapfunyika gafite agakoresho ko gusukura 25T
.Igisubizo 25T
Igisubizo cya .B 1
.Ipaki ishyirwamo 1

IBIKORESHO BIRAKENEWE ARIKO NTIBYATANGWA
Agasanduku ko gukusanya ingero, igihe

ICYITEGEREREZO CYO GUKORESHA NO KUBIKA
1. Ingero zapimwe zishobora kuba serum, heparin anticoagulant plasma cyangwa EDTA anticoagulant plasma.
2. Dukurikije uburyo busanzwe bwo gukusanya icyitegererezo. Icyitegererezo cya serum cyangwa plasma gishobora kubikwa muri firigo kuri 2-8°C mu gihe cy'iminsi 7 no kubikwa munsi ya -15°C mu gihe cy'amezi 6.
3. Ingero zose zirinda gukonjesha no gushonga.

UBURYO BWO GUKORA IKIGANIRO
Uburyo bwo gupima igikoresho reba igitabo cy'amabwiriza agenga ikoreshwa ry'imyunyungugu. Uburyo bwo gupima reagent ni ubu bukurikira
1. Shyira ku ruhande ibikoresho byose bikoreshwa mu gupima no mu gupima ingero ku bushyuhe bw'icyumba.
2. Fungura Portable Immune Analyzer (WIZ-A101), andika ijambo ry'ibanga ryinjira muri konti ukurikije uburyo bw'imikorere y'igikoresho, hanyuma winjize aho ureba.
3.Skena kode y'isuzuma kugira ngo wemeze ikintu cy'ikizamini.
4. Kura ikarita yo gupima mu gafuka k'ifishi.
5. Shyiramo ikarita y'ikizamini mu mwanya w'ikarita, skana kode ya QR, hanyuma umenye ikintu cy'ikizamini.
6. Shyiramo serum cyangwa plasma ya 30μL mu gisubizo cya A, hanyuma uvange neza.
7. Shyiramo umuti wa 50μL B mu ruvange rwavuzwe haruguru, hanyuma uvange neza.
8.Reka uruvange rube iminota 15.
9. Shyiramo uruvange rwa 80μL kugira ngo ufate urugero rw'ikarita.
10. Kanda kuri buto "ikizamini gisanzwe", nyuma y'iminota 10, igikoresho kizahita kibona ikarita y'ikizamini, gishobora gusoma ibisubizo bivuye kuri ecran y'igikoresho, no kwandika/gucapa ibisubizo by'ikizamini.
11. Reba amabwiriza ya Portable Immune Analyzer (WIZ-A101).

INDANGAGACIRO ZITEGANYIJWE
25-(OH)VD intera isanzwe: 30-100ng/mL
Birasabwa ko buri laboratwari ishyiraho urwego rwayo rusanzwe rugaragaza umubare w’abarwayi bayo.

IBISUBIZO BY'IBIGERAGEZO N'UBUSOBANURO
.Amakuru yavuzwe haruguru ni igihe cyo gusuzuma amakuru y’iyi kit, kandi birasabwa ko buri laboratwari igomba gushyiraho igihe cyo gusuzuma amakuru ajyanye n’akamaro k’ubuvuzi ku baturage bo muri aka gace.
.Ingano ya 25-(OH)VD iri hejuru y’urugero rw’ifatizo, kandi impinduka z’umubiri cyangwa igisubizo cy’imihangayiko ntizigomba kurebwa. Mu by’ukuri, bigomba guhuza ibimenyetso by’indwara.
Ibisubizo by'ubu buryo bireba gusa urwego rw'ifatizo rwashyizweho n'ubu buryo, kandi ibisubizo ntibigereranywa neza n'ubundi buryo.
Izindi mpamvu zishobora gutera amakosa mu bisubizo byo kuvumbura, harimo impamvu za tekiniki, amakosa mu mikorere n'izindi mpamvu z'icyitegererezo.

UBUBIKO N'UBUTABE
.Iyi mashini ishobora kumara amezi 18 ikoreshwa kuva ku itariki yakozwe. Bika ibikoresho bitakoreshejwe kuri dogere selisiyusi 2-30. NTUBIKORESHE. Ntukabikoreshe nyuma y'itariki ntarengwa.
Ntugafungure agafuka gafunze kugeza igihe uzaba witeguye gukora ikizamini, kandi ikizamini gikoreshwa rimwe gusa ni byiza ko gikoreshwa mu gihe gikenewe (ubushyuhe 2-35℃, ubushuhe 40-90%) mu minota 60 vuba bishoboka.
.Icyitegererezo cy'umuti uvangwa n'amazi gikoreshwa ako kanya nyuma yo gufungurwa.

IMIBURO N'IBYITONDERWA
.Isanduku igomba kuba ifunze kandi irinzwe ubushuhe.
Ingero zose z’ibyavuye mu bushakashatsi zigomba kwemezwa n’ubundi buryo.
Ibipimo byose bigomba gufatwa nk'ibintu bishobora kwanduza.
NTUKORESHE reagent yarengeje igihe.
NTUGAHINDURE ibyuma bitanga ubuziranenge hagati y'ibikoresho bifite ubwoko butandukanye..
NTUGAKORESHE gukoresha amakarita yo gupima n'ibindi bikoresho byo gukoresha mu gihe cyo kuyakoresha.
.Gukoresha nabi, icyitegererezo gikabije cyangwa gito bishobora gutuma ibisubizo bihinduka.

LKWIGANA
Nkuko bigenda ku isuzuma ryose rikoresha uturemangingo tw’imbeba, birashoboka ko uturemangingo tw’imbeba (HAMA) twangiritse mu gipimo. Ingero z’abarwayi bahawe imiti y’uturemangingo tw’imbeba kugira ngo bapimwe cyangwa bavurwe zishobora kuba zirimo HAMA. Ingero nk’izo zishobora gutera ibisubizo bitari byo by’ukuri cyangwa bitari ukuri.
Ibi bisubizo ni ibyo kwitabwaho mu buvuzi gusa, ntabwo bigomba kuba ishingiro ryonyine ryo gusuzuma no kuvura abarwayi, uburyo bwo kuvura abarwayi bugomba kwitabwaho mu buryo bwuzuye hamwe n'ibimenyetso byabo, amateka y'ubuzima bwabo, ubundi buryo bwo gusuzuma muri laboratwari, uko ubuvuzi bwaba buhagaze, ibyorezo by'indwara n'andi makuru.
Iyi reagent ikoreshwa gusa mu gupima amaraso mu maraso no mu maraso. Ishobora kutabona igisubizo nyacyo iyo ikoreshejwe mu bindi bipimo nk'amacandwe n'inkari n'ibindi.

IBIRANGA IMIKORERE

RIBIREBANA

1.Hansen JH, et al.HAMA Interference hamwe n'ibizamini by'imyuka bishingiye ku murine monoclonal antibody [J].J bya Clin Immunoassay, 1993,16:294-299.
2.Levinson SS. Imiterere y'uturemangingo tw'umubiri duto duto n'uruhare mu gutera ikibazo cy'ubudahangarwa bw'umubiri [J].J ya Clin Immunoassay, 1992,15:108-114.

Urufunguzo rw'ibimenyetso byakoreshejwe:

	Igikoresho cyo kwa muganga gisuzuma indwara muri Vitro
	Uruganda
	Bika ahantu hari ubushyuhe buri hagati ya 2 na 30°C
	Itariki izarangiriraho
	Ntukongere gukoresha
	UBWITONDE
	Reba amabwiriza yo gukoresha

Xiamen Wiz Biotech CO.,LTD
Aderesi: Igorofa ya 3-4, Inyubako ya NO.16, Ishuri Rikuru ry’Ubuvuzi bw’Ibinyabuzima, 2030 Wengjiao West Road, Akarere ka Haicang, 361026, Xiamen, Ubushinwa
Terefone: +86-592-6808278
Fakisi: +86-592-6808279