NHEYO

Kamudzira kemudziyo wekuyedza kakaputirwa neanti EV71 antibody panzvimbo yekuyedza uye anti mbudzana IgG antibody panzvimbo yekudzora. Mapepa eLable pad anoputirwa ne fluorescence yakanyorwa kuti anti EV71 antibody uye tsuro IgG pachine nguva. Pakuyedza sampuro yakanaka, EV71 antigen iri mumuenzaniso inosanganiswa ne fluorescence yakanyorwa kuti anti EV71 antibody, uye inoumba musanganiswa we immune immune. Pasi pekushanda kwe chromatography, kuyerera kwakaomarara munzira yepepa rinonyudza, kana complex yapfuura nzvimbo yekuyedza, yakabatana ne anti EV71 coating antibody, inoumba complex itsva.

Kana iri negative, sampuro haina enterovirus 71 IgM antibody, zvekuti immune complex haigone kuumbwa. Hapazovi nemutsara mutsvuku munzvimbo yekuona (T). Hazvina mhosva kuti Enterovirus 71 IgM antibody iripo here musampuli kana kuti kwete, yasara colloidal gold-labeled gold antibody anti-human IgM monoclonal antibody uye goat anti-mouse IgG antibody yakaputirwa munzvimbo yekudzora kunaka (C) inosunga. Zvadaro ma agglutinates anova neruvara munzvimbo yekudzora kunaka, uye mutsetse mutsvuku uchaonekwa mu (C). Mutsetse mutsvuku ndiwo mwero unoonekwa munzvimbo yekudzora kunaka (C) wekutonga kana paine samples dzakakwana uye kana maitiro e chromatography ari akajairwa. Inoshandiswawo semwero wemukati we reagents.

Maitiro Ekuyedza:

1. Mienzaniso yakaedzwa inogona kunge iri ropa rose, kusanganisira ropa remutsinga kana ropa rekunze. Ropa rose harigone kuchengetwa mushure mekutorwa. Ndinofanira kushandiswa nekukurumidza mushure mekutorwa.

2. Samples dzemuropa dzinounganidzwa pasina utachiona zvichienderana nemaitiro akajairwa. Serum isina kupisa haigone kushandiswa. Hazvikurudzirwi kushandisa serum ine mafuta akawanda, ine turbid kana yakasvibiswa. Zvidimbu zviri muropa. Uye mvura inonaya inokanganisa mhedzisiro yebvunzo, samples dzakadaro dzinofanira kucheneswa kana kusefa dzisati dzashandiswa.

3. Mienzaniso yakaedzwa inogona kuva heparin, Sodium citrate kana EDTA anticoagulant plasma.

4. Zvinoenderana nemaitiro akajairwa ekuunganidza sampuro. Sampuro yeropa kana yeropa inogona kuchengetwa mufiriji pa2-8℃ kwemazuva matatu uye kucheneswa pasi pe -15°C kwemwedzi mitatu.

5. Mienzaniso yese inodzivirira kutonhora nekunyunguduka.