Ikhithi yokuxilonga yeI-Luteinizing Hormoneuvavanyo lwe-fluorescence immunochromatographic)
Ukusetyenziswa kokuxilongwa kwi-vitro kuphela
Nceda ufunde le phakheji ngononophelo phambi kokuba uyisebenzise kwaye ulandele imiyalelo ngokungqongqo. Ukuthembeka kweziphumo zovavanyo akunakuqinisekiswa ukuba kukho naluphi na uphambuko kwimiyalelo ekwesi sithuba sephakheji.

UKUSETYENZISWA OKUJOLISWEYO
Ikhithi yokuxilonga yeLuteinizing Hormone (uvavanyo lwe-fluorescence immunochromatographic) luvavanyo lwe-fluorescence immunochromatographic lokufumanisa ubungakanani beLuteinizing Hormone (LH) kwi-serum okanye kwi-plasma yomntu, esetyenziswa kakhulu kuvavanyo lomsebenzi we-endocrine ye-pituitary. Zonke iisampulu ezilungileyo kufuneka ziqinisekiswe zezinye iindlela. Olu vavanyo lwenzelwe ukusetyenziswa ziingcali zonyango kuphela.

ISISHWANKATHELO

I-Luteinizing hormone (LH) yi-glycoprotein enobunzima beemolekyuli obumalunga nama-30,000 eDalton, eveliswa yi-anterior pituitary. Uxinzelelo lwe-LH lunxulumene kakhulu nokuvuthwa kwamaqanda, kwaye incopho ye-LH iqikelelwa ukuba yiyure ezingama-24 ukuya kuma-36 zokuvuthwa. Ke ngoko, ixabiso eliphezulu le-LH linokubekwa esweni ngexesha lomjikelo wokuya esikhathini ukuze kuchongwe ixesha elifanelekileyo lokukhulelwa. Umsebenzi ongaqhelekanga we-endocrine kwi-pituitary gland unokubangela ukungahambelani kokukhutshwa kwe-LH. Uxinzelelo lwe-LH lungasetyenziselwa ukuvavanya umsebenzi we-pituitary endocrine. I-Diagnostic Kit isekelwe kwi-immunochromatography kwaye inokunika iziphumo kwimizuzu eli-15.

UMGAQO WENKQUBO

I-membrane yesixhobo sovavanyo igqunywe nge-anti-LH antibody kwindawo yovavanyo kunye ne-anti-rabbit IgG antibody yebhokhwe kwindawo yokulawula. I-Lable pad igqunywe nge-fluorescence ebhalwe nge-anti-LH antibody kunye ne-rabbit IgG kwangaphambili. Xa kuvavanywa isampuli elungileyo, i-antigen ye-LH kwisampuli idibana ne-anti-LH antibody ebhalwe nge-fluorescence, kwaye yenze umxube wokuzikhusela. Phantsi kwesenzo se-immunochromatography, ukuhamba okuntsonkothileyo kwicala lephepha elifunxayo, xa i-complex idlula kwindawo yovavanyo, idibene ne-anti-LH coating antibody, yenza i-complex entsha. Inqanaba le-LH lihambelana kakuhle nesignali ye-fluorescence, kwaye uxinzelelo lwe-LH kwisampuli lunokufunyanwa ngovavanyo lwe-fluorescence immunoassay.

IZINTO EZINIKEZELWAYO NEZIXHOBO

Izixhobo zephakheji ze-25T：

Ikhadi lovavanyo ngalinye lifakwe ifoyile ngesixhobo sokucoca i-desiccant esingu-25T
.Iisampulu zokuxuba
.Iphakheji efakiweyo

IZIXHOBO EZIFUNEKAYO KODWA AZINIKELWANGA
Isikhongozeli sokuqokelela isampuli, isibali-xesha

IMFUNDO YOKUQOKELELA NOKUGCINA IMIZEKELO
1.Iisampulu ezivavanyiweyo zinokuba yi-serum, i-heparin anticoagulant plasma okanye i-EDTA anticoagulant plasma.
2. Ngokweendlela eziqhelekileyo zokuqokelela isampuli. Isampuli ye-serum okanye yeplasma ingagcinwa efrijini kwi-2-8℃ iintsuku ezisi-7 kwaye igcinwe ngaphantsi kwe-15°C iinyanga ezi-6.
3.Zonke iisampuli ziphephe imijikelo yokuqandisa nokunyibilikisa.

INKQUBO YOVAVANYO

Inkqubo yovavanyo lwesixhobo jonga incwadi yemiyalelo ye-immunoanalyzer. Inkqubo yovavanyo lwe-reagent yile ilandelayo
1. Beka ecaleni zonke ii-reagents kunye neesampuli ukuya kubushushu begumbi.
2.Vula i-Portable Immune Analyzer (WIZ-A101), faka igama lokugqitha le-akhawunti ngokwendlela yokusebenza kwesixhobo, uze ufake i-interface yokubona.
3.Skena ikhowudi yokuchonga ukuze uqinisekise into yovavanyo.
4.Khupha ikhadi lovavanyo kwingxowa yefoyile.
5.Faka ikhadi lovavanyo kwindawo yokubeka ikhadi, skena ikhowudi ye-QR, uze umisele into yovavanyo.
6. Yongeza isampuli yeserum okanye iplasma engama-20μL kwisampuli ye-diluent, uze udibanise kakuhle.
7. Yongeza isisombululo sesampulu se-80μL kwisampulu yomthombo wekhadi.
8.Cofa iqhosha elithi “uvavanyo oluqhelekileyo”, emva kwemizuzu eli-15, isixhobo siza kuzibona ngokuzenzekelayo ikhadi lovavanyo, singafunda iziphumo kwisikrini sokubonisa isixhobo, size sirekhode/siprinte iziphumo zovavanyo.
9. Jonga imiyalelo ye-Portable Immune Analyzer (WIZ-A101).

IZIPHUMO ZOVAVANYO NOKUTOLIKWA

.Idatha engentla lixesha elimiselweyo lokufumana idatha yale khithi, kwaye kucetyiswa ukuba ilabhoratri nganye imisele ixesha elimiselweyo lokubaluleka kweklinikhi okufanelekileyo kubemi abakulo mmandla.
.Uxinzelelo lwe-LH luphezulu kunoluhlu olufunekayo, kwaye utshintsho lwe-physiological okanye impendulo yoxinzelelo kufuneka lungabandakanywa. Enyanisweni, okungaqhelekanga, kufuneka kudityaniswe ukuxilongwa kweempawu zeklinikhi.
Iziphumo zale ndlela zisebenza kuphela kuluhlu lwesalathiso olumiselwe yile ndlela, kwaye iziphumo azifani ngokuthe ngqo nezinye iindlela.
Ezinye izinto zinokubangela iimpazamo kwiziphumo zokufumanisa, kuquka izizathu zobugcisa, iimpazamo zokusebenza kunye nezinye izinto zesampulu.

UKUGCINWA NOKUZINZISA
1. Le khithi ihlala iinyanga ezili-18 ukususela kumhla wokwenziwa kwayo. Gcina ezi khithi zingasetyenziswanga kwi-2-30°C. SUKUZIQANDA. Musa ukuzisebenzisa emva komhla wokuphelelwa.
2. Musa ukuvula isikhwama esivaliweyo ude ulungele ukwenza uvavanyo, kwaye uvavanyo olusetyenziswa kanye lucetyiswa ukuba lusetyenziswe phantsi kweemeko ezifunekayo (ubushushu obuyi-2-35℃, umswakama oyi-40-90%) kwimizuzu engama-60 ngokukhawuleza okukhulu.
3. Isampulu yomxube isetyenziswa kwangoko emva kokuba ivuliwe.

IZILUMKISO NEZILUMKISO
.Ikhithi kufuneka ivalwe kwaye ikhuselwe kumswakama.
Zonke iisampulu ezilungileyo maziqinisekiswe ngezinye iindlela.
Zonke iisampulu maziphathwe njengezingcolisi ezinokubakho.
.MUSA ukusebenzisa i-reagent ephelelwe lixesha.
AKUFUNWA ukutshintshanisa ii-reagents phakathi kweekiti ezinenombolo eyahlukileyo.
.MUSA ukusebenzisa kwakhona amakhadi ovavanyo kunye nazo naziphi na izixhobo ezilahlwayo.
.Ukusetyenziswa gwenxa, isampuli egqithisileyo okanye encinci kunokubangela ukuphambuka kweziphumo.

LUKULINGANISA
.Njengakweyiphi na i-assay esebenzisa ii-antibodies zegundane, kunokwenzeka ukuba kubekho ukuphazamiseka kwee-antibodies ze-anti-mouse (HAMA) kwi-sampuli. Iisampuli ezivela kwizigulana ezifumene amalungiselelo e-monoclonal antibodies ukuze zixilongwe okanye zonyango zinokuba ne-HAMA. Ezi sampuli zinokubangela iziphumo ezilungileyo okanye ezimbi.
.Esi siphumo sovavanyo sesokubhekisela kwiklinikhi kuphela, akufuneki sisebenze njengesiseko sodwa sokuxilongwa kweklinikhi kunye nonyango, ulawulo lweklinikhi lwezigulane kufuneka luqwalaselwe ngokupheleleyo kunye neempawu zalo, imbali yezonyango, olunye uvavanyo lwelabhoratri, impendulo yonyango, i-epidemiology kunye nolunye ulwazi.
.Le reagent isetyenziswa kuphela kuvavanyo lwe-serum kunye ne-plasma. Isenokungafumani ziphumo zichanekileyo xa isetyenziswa kwezinye iisampuli ezifana namathe nomchamo njl.

IIMPAWU ZOKUSEBENZA

IIREFERENSI
1.Hansen JH, et al. Ukuphazamiseka kwe-HAMA kwi-Murine Monoclonal Antibody-Based Immunoassays[J].J ye-Clin Immunoassay,1993,16:294-299.
2.Levinson SS. Uhlobo lwee-Antibodies ze-Heterophilic kunye nendima ekuphazamisekeni kwe-Immunoassay[J].J ye-Clin Immunoassay,1992,15:108-114.

Isitshixo seempawu ezisetyenzisiweyo:

	Isixhobo Sonyango Sokuxilonga se-In Vitro
	Umenzi
	Gcina kwi-2-30℃
	Umhla WOKUPHELELWA
	Musa Ukuphinda Usebenzise
	ISILUMKISO
	Jonga Imiyalelo Yokusetyenziswa

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