ISIMISO

Ulwelwesi lwedivayisi yokuhlola lumbozwe nge-antibody ye-EV71 endaweni yokuhlola kanye ne-antibody yembuzi ye-anti rabbit IgG endaweni yokulawula. Iphedi yelebula imbozwe nge-antibody ye-antiEV71 ebhalwe nge-fluorescence kanye ne-IgG yenogwaja kusengaphambili. Lapho kuhlolwa isampula enhle, i-antigen ye-EV71 kusampula ihlangana ne-antibody ye-antiEV71 ebhalwe nge-fluorescence, bese yakha ingxube yokuzivikela komzimba. Ngaphansi kwesenzo se-chromatography, ukugeleza okuyinkimbinkimbi kuya ohlangothini lwephepha elimuncayo, lapho i-complex idlula endaweni yokuhlola, ihlanganiswe ne-antibody ye-antiEV71 coating, yakha i-complex entsha.

Uma ingenayo i-antibody ye-enterovirus 71 IgM, ngakho-ke i-immune complex ayikwazi ukwakheka. Ngeke kube khona umugqa obomvu endaweni yokuthola (T). Kungakhathaliseki ukuthi i-antibody ye-Enterovirus 71 IgM ikhona yini kusampula noma cha, i-antibody yegundane elimele i-IgM monoclonal yegundane elibhalwe ngegolide kanye ne-antibody ye-IgG yegundane elimele igundane elimbozwe endaweni yokulawula ikhwalithi (C) ziyabopha. Ngemuva kwalokho ama-agglutinates athuthukisa umbala endaweni yokulawula ikhwalithi, bese umugqa obomvu uzovela ku-(C). Umugqa obomvu uyindinganiso ebonakala endaweni yokulawula ikhwalithi (C) yokunquma ukuthi kukhona amasampula anele nokuthi inqubo ye-chromatography ijwayelekile yini. Isetshenziswa futhi njengendinganiso yokulawula yangaphakathi yama-reagents.

Inqubo Yokuhlola:

1. Amasampula ahlolwe angaba yigazi eliphelele, okuhlanganisa igazi elisemithambo yegazi noma igazi elingaphandle. Igazi eliphelele alikwazi ukugcinwa ngemva kokuqoqwa. Kufanele lisetshenziswe ngokushesha ngemva kokuqoqwa.

2. Amasampula e-serum aqoqwa ngendlela engenamagciwane ngokwezindlela ezijwayelekile. I-serum engasebenzisi ukushisa ayikwazi ukusetshenziswa. Akukhuthazwa ukusebenzisa i-serum ene-lipemic, turbid noma engcolile. Izinto ezisanhlamvu ku-serum. Futhi imvula izothinta imiphumela yokuhlolwa, amasampula anjalo kufanele afakwe ku-centrifuge noma ahlungwe ngaphambi kokusetshenziswa.

3. Amasampula ahlolwe angaba yi-heparin, i-Sodium citrate noma i-EDTA anticoagulant plasma.

4. Ngokwezindlela ezijwayelekile zokuqoqa isampula. Isampula ye-serum noma ye-plasma ingagcinwa efrijini ku-2-8℃ izinsuku ezi-3 kanye nokugcinwa ngaphansi kuka-15°C izinyanga ezi-3.

5. Zonke izibonelo zigwema imijikelezo yokuqandisa nokuncibilikisa.