Kit ɗin Bincike donHormone mai narkewagwajin immunochromatographic na fluorescence)
Don amfani da ganewar asali kawai a cikin vitro
Da fatan za a karanta wannan fakitin da aka saka a hankali kafin amfani kuma a bi umarnin sosai. Ba za a iya tabbatar da ingancin sakamakon gwaji ba idan akwai wata matsala daga umarnin da ke cikin wannan fakitin.

AN YI NIYYA DA AMFANI
Kayan Bincike don Luteinizing Hormone (gwajin fluorescence immunochromatographic) gwajin fluorescence immunochromatographic ne don gano adadi na Luteinizing Hormone (LH) a cikin jini ko plasma na ɗan adam, wanda galibi ana amfani da shi wajen kimanta aikin glandar pituitary endocrine. Dole ne a tabbatar da duk wani samfurin da ya nuna yana da kyau ta wasu hanyoyin. Wannan gwajin an yi shi ne don amfanin ƙwararru a fannin kiwon lafiya kawai.

TAƘAITACCE

Hormone na Luteinizing (LH) wani sinadari ne mai sinadarin glycoprotein mai nauyin kwayoyin halitta kimanin Dalton 30,000, wanda pituitary na gaba ke samarwa. Yawan LH yana da alaƙa da ovulation na ovulation na ovulation, kuma ana hasashen cewa kololuwar LH zai kasance awanni 24 zuwa 36 na ovulation. Saboda haka, ana iya sa ido kan kololuwar LH a lokacin zagayowar haila don tantance lokacin da ya dace na ɗaukar ciki. Rashin aikin endocrine mara kyau a cikin glandar pituitary na iya haifar da rashin daidaituwar fitar LH. Ana iya amfani da yawan LH don tantance aikin endocrine na pituitary. Kayan Bincike ya dogara ne akan immunochromatography kuma yana iya bayar da sakamako cikin mintuna 15.

KA'IDUN TSARIN

An shafa membrane na na'urar gwajin da maganin rigakafi na LH a yankin gwaji da kuma maganin rigakafi na IgG na akuya a yankin sarrafawa. Ana shafa murfin label da aka yiwa lakabi da maganin rigakafi na LH da kuma maganin rigakafi na zomo IgG a gaba. Lokacin gwada samfurin tabbatacce, maganin rigakafi na LH a cikin samfurin yana haɗuwa da maganin rigakafi na LH mai lakabin fluorescence, kuma yana samar da cakuda garkuwar jiki. A ƙarƙashin aikin immunochromatography, kwararar hadaddun a cikin jagorancin takardar sha, lokacin da hadaddun ya wuce yankin gwaji, an haɗa shi da maganin rigakafi na LH mai rufi, yana samar da sabon hadaddun. Matsayin LH yana da alaƙa mai kyau da siginar haske, kuma ana iya gano yawan LH a cikin samfurin ta hanyar gwajin immunoassay na fluorescence.

MASU YIN REAGENTS DA KAYAN DA AKA KAWO

Kayan aikin fakitin 25T:

.Katin gwaji daban-daban an saka shi a cikin jaka mai ɗauke da ruwan zafi 25T
.Samfurin masu narkewa
.Saka fakitin

ANA BUKATAR KAYAN AIKI AMMA BA A BA DA SU BA
Samfurin tarin akwati, mai ƙidayar lokaci

TARIN SAMFURI DA AJIYEWA
1. Samfuran da aka gwada na iya zama na jini, heparin anticoagulant plasma ko EDTA anticoagulant plasma.
2. Dangane da dabarun da aka saba amfani da su, ana iya ajiye samfurin jini ko na plasma a cikin firiji a zafin 2-8℃ na tsawon kwanaki 7, kuma ana iya ajiye shi a wuri mai sanyi a ƙasa da -15°C na tsawon watanni 6.
3. Duk samfurin da aka yi amfani da shi don guje wa daskarewa da narkewar zagayowar.

Tsarin Gwaji

Tsarin gwajin kayan aikin duba littafin immunoanalyzer. Tsarin gwajin reagent shine kamar haka:
1. A ajiye dukkan sinadaran da aka yi amfani da su da kuma samfuran da aka yi amfani da su a zafin ɗaki.
2. Buɗe Mai Nazarin Jiki Mai Ɗaukewa (WIZ-A101), shigar da kalmar sirri ta asusun bisa ga hanyar aiki ta kayan aikin, sannan ka shigar da hanyar ganowa.
3. Duba lambar haƙoran don tabbatar da abin gwajin.
4. Cire katin gwaji daga jakar foil.
5. Saka katin gwaji a cikin ramin katin, duba lambar QR, sannan ka tantance abin gwajin.
6. Ƙara samfurin serum ko plasma 20μL a cikin samfurin mai narkewa, sannan a gauraya sosai.
7. Ƙara maganin samfurin 80μL don yin samfurin rijiyar katin.
8. Danna maɓallin "gwaji na yau da kullun", bayan mintuna 15, kayan aikin zai gano katin gwaji ta atomatik, zai iya karanta sakamakon daga allon nuni na kayan aikin, kuma ya yi rikodin/buga sakamakon gwajin.
9. Duba umarnin Portable Immune Analyzer (WIZ-A101).

SAKAMAKON GWAJI DA FASSARAR

. Bayanan da ke sama sune tazarar da aka kafa don bayanan gano wannan kayan aiki, kuma ana ba da shawarar cewa kowane dakin gwaje-gwaje ya kamata ya kafa tazarar da ta dace don mahimmancin asibiti na yawan jama'a a wannan yanki.
. Yawan LH ya fi na ma'aunin tunani, kuma ya kamata a cire canje-canje a cikin jiki ko amsawar damuwa. Hakika, ya kamata a haɗa ganewar alamun asibiti.
.Sakamakon wannan hanyar yana aiki ne kawai ga kewayon tunani da wannan hanyar ta kafa, kuma sakamakon ba ya kama da na sauran hanyoyi kai tsaye.
.Wasu dalilai kuma na iya haifar da kurakurai a sakamakon ganowa, gami da dalilan fasaha, kurakuran aiki da sauran abubuwan samfurin.

Ajiya da kwanciyar hankali
1. Kayan aikin yana da tsawon watanni 18 daga ranar da aka ƙera shi. Ajiye kayan da ba a yi amfani da su ba a zafin 2-30°C. KAR A DASKARE. Kada a yi amfani da su fiye da ranar karewa.
2. Kar a buɗe jakar da aka rufe har sai kun shirya yin gwaji, kuma ana ba da shawarar a yi amfani da gwajin amfani da shi sau ɗaya a ƙarƙashin yanayin da ake buƙata (zafin jiki 2-35℃, danshi 40-90%) cikin mintuna 60 da sauri.
3. Ana amfani da samfurin maganin shafawa nan da nan bayan an buɗe shi.

GARGAƊI DA HANKALI
.Ya kamata a rufe kayan aikin kuma a kare shi daga danshi.
Duk samfuran da aka tabbatar za a tabbatar da su ta wasu hanyoyi.
.Duk samfuran za a yi musu la'akari da su a matsayin masu iya gurɓata muhalli.
.KADA a yi amfani da maganin da ya ƙare.
. BA a musanya sinadaran da ke cikin kayan aiki da sauran kayan aiki ba.
.KADA a sake amfani da katunan gwaji da duk wani kayan haɗi da za a iya yarwa.
.Rashin aiki daidai, ko wuce gona da iri ko ƙarancin samfur na iya haifar da karkacewar sakamako.

LKWAIKWAYO
Kamar yadda yake da kowace gwaji da ake amfani da maganin rigakafi na linzamin kwamfuta, akwai yiwuwar tsangwama daga ƙwayoyin rigakafi na ɗan adam (HAMA) a cikin samfurin. Samfuran marasa lafiya waɗanda aka yi musu shirye-shiryen ƙwayoyin rigakafi na monoclonal don ganewar asali ko magani na iya ƙunsar HAMA. Irin waɗannan samfuran na iya haifar da sakamako mara kyau ko mara kyau na ƙarya.
.Wannan sakamakon gwajin an yi shi ne kawai don bayanin asibiti, bai kamata ya zama tushen ganewar asali da magani na asibiti ba, ya kamata a yi la'akari da kulawar marasa lafiya sosai tare da alamominsa, tarihin likita, sauran gwajin dakin gwaje-gwaje, amsawar magani, ilimin cututtuka da sauran bayanai.
.Wannan sinadarin ana amfani da shi ne kawai don gwaje-gwajen jini da na jini. Yana iya ba zai sami sakamako mai kyau ba idan aka yi amfani da shi ga wasu samfura kamar su yau da fitsari da sauransu.

HALAYEN AYYUKA

NAZARI
1. Hansen JH, da sauransu. Tsoma bakin HAMA ga gwajin rigakafi na Murine Monoclonal Antibody-based [J].J na Clin Immunoassay, 1993,16:294-299.
2. Levinson SS. Yanayin Kwayoyin Haɗaka Masu Yawa da Matsayinsu a Cikin Tsangwama Kan Tsarin Immunoassay[J].J na Clin Immunoassay, 1992,15:108-114.

Maɓallin alamomin da aka yi amfani da su:

	Na'urar Lafiya ta In Vitro Diagnostic
	Mai ƙera
	Ajiye a 2-30℃
	Ranar Karewa
	Kada a sake amfani da shi
	HANKALI
	Duba Umarnin Amfani

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