KA'IDA

An shafa membrane na na'urar gwajin da maganin rigakafi na EV71 a yankin gwaji da kuma maganin rigakafi na IgG na maganin akuya a yankin sarrafawa. Ana shafa murfin label da maganin rigakafi na EV71 mai lakabin fluorescence da kuma maganin rigakafi na zomo IgG a gaba. Lokacin gwada samfurin tabbatacce, maganin rigakafi na EV71 a cikin samfurin yana haɗuwa da maganin rigakafi na EV71 mai lakabin fluorescence, kuma yana samar da cakuda garkuwar jiki. A ƙarƙashin aikin chromatography, kwararar hadaddun a cikin jagorancin takardar sha, lokacin da hadaddun ya wuce yankin gwaji, ya haɗu da maganin rigakafi na EV71 mai rufi, ya samar da sabon hadaddun.

Idan ba shi da kyau, samfurin ba ya ɗauke da kwayar cutar enterovirus 71 IgM, don haka ba za a iya samar da hadaddun garkuwar jiki ba. Ba za a sami layi ja a yankin da aka gano (T) ba. Ko da akwai kwayar cutar Enterovirus 71 IgM a cikin samfurin ko a'a, sauran kwayar cutar beraye mai alamar zinare ta colloidal anti-human IgM monoclonal antibody da kuma kwayar cutar beraye mai hana linzamin akuya IgG da aka lulluɓe a yankin kula da inganci (C) za su haɗu. Sannan ƙwayoyin cuta suna samun launi a yankin kula da inganci, kuma layin ja zai bayyana a cikin (C). Layin ja shine ma'aunin da ke bayyana a yankin kula da inganci (C) don tantance ko akwai isassun samfura da kuma ko tsarin chromatography na al'ada ne. Haka kuma ana amfani da shi azaman ma'aunin kula da ciki don reagents.

Tsarin Gwaji:

1. Samfuran da aka gwada na iya zama jini gaba ɗaya, har da jinin jijiyar jiki ko jinin gefe. Ba za a iya adana cikakken jinin bayan an tattara ba. Ya kamata a yi amfani da ni jim kaɗan bayan an tattara.

2. Ana tattara samfuran jini ta hanyar septic bisa ga dabarun da aka saba. Ba za a iya amfani da maganin da aka kunna da zafi ba. Ba a ba da shawarar a yi amfani da maganin lipemic, turbid ko gurbatacce ba. Ƙwayoyin da ke cikin maganin. Kuma ruwan sama zai shafi sakamakon gwajin, ya kamata a tace su ko a tace su kafin amfani.

3. Samfuran da aka gwada na iya zama heparin, sodium citrate ko EDTA anticoagulant plasma.

4. Dangane da dabarun da aka saba amfani da su, ana iya ajiye samfurin jini ko na plasma a cikin firiji a zafin 2-8℃ na tsawon kwanaki 3, sannan a ajiye shi a wuri mai sanyi a ƙasa da -15°C na tsawon watanni 3.

5. Duk samfurin da aka yi amfani da shi don guje wa daskarewa da narkewar zagayowar.