TE MATUA

Kua pania te kiriuhi o te taputapu whakamātautau ki te paturopi anti-EV71 i te rohe whakamātautau, me te paturopi anti-rāpeti koati IgG i te rohe whakahaere. Kua pania ngā papa tapanga ki te paturopi anti-EV71 me te IgG rāpeti kua tohua ki te fluorescence i mua i te whakamātautau. I te whakamātautau i te tauira pai, ka honoa te antigen EV71 i roto i te tauira ki te paturopi anti-EV71 kua tohua ki te fluorescence, ā, ka hanga he ranunga ārai mate. I raro i te mahi a te chromatography, ka rere te matatini i te ahunga o te pepa mimiti, ā, ina whiti te matatini i te rohe whakamātautau, ka honoa ki te paturopi paninga anti-EV71, ka hanga he matatini hou.

Mena he kino, kāore he paturopi enterovirus 71 IgM i roto i te tauira, nō reira kāore e taea te hanga i te matatini ārai mate. Kāore he rārangi whero i te rohe kitenga (T). Ahakoa kei roto i te tauira te paturopi Enterovirus 71 IgM, ka herea te paturopi monoclonal IgM tangata kiore kua tohua ki te koura me te paturopi IgG kiore koati kua pania ki te rohe whakahaere kounga (C). Kātahi ka puta he tae i roto i te rohe whakahaere kounga ngā aglutinates, ā, ka puta te rārangi whero i roto i te (C). Ko te rārangi whero te paerewa e puta ana i roto i te rohe whakahaere kounga (C) hei whakatau mena he nui ngā tauira, me te mea he noa te tukanga chromatography. Ka whakamahia hoki hei paerewa whakahaere ā-roto mō ngā matū.

Tukanga Whakamātautau:

1. Ka taea te whakamahi i te toto katoa, tae atu ki te toto uaua, te toto taha rānei, hei tauira whakamātautau. Kāore e taea te penapena i te toto katoa i muri i te kohikohinga. Me whakamahi wawe tonu i muri i te kohikohinga.

2. Ka kohia ngā tauira toto mā te kore-whakamātautau, kia rite ki ngā tikanga paerewa. Kāore e taea te whakamahi i te toto kua kore e wera. Kāore i te tūtohutia kia whakamahia he toto kua mimiti, kua poke, kua poke rānei. Kei roto i te toto ngā matūriki. Ā, ka pāngia ngā hua o te whakamātautau e te ua, me whakatōtō, me tātari rānei aua tauira i mua i te whakamahinga.

3. Ko ngā tauira i whakamatautauria he heparin, he konutai citrate, he EDTA rānei te plasma anticoagulant.

4. E ai ki ngā tikanga paerewa, kohia he tauira. Ka taea te pupuri i te tauira toto, i te tauira plasma rānei ki roto i te pouaka whakamātao i te 2-8℃ mō ngā rā e 3, ā, ka taea te pupuri i te tauira i raro i te -15°C mō ngā marama e 3.

5. Kaua ngā tauira katoa e huri i te huka me te rewa.