IHAME

Agace k'igikoresho cyo gupima gatwikiriwe na antibody ya anti EV71 ku gace k'igeragezwa n'antibody ya antigum ya IgG y'ihene ku gace k'igenzura. Udupapuro two gupima dutwikiriwe na antibody ya anti EV71 yanditseho fluorescence na antibody ya IgG y'urukwavu mbere y'igihe. Mu gusuzuma icyitegererezo cyagaragaje ko ari cyiza, antigen ya EV71 iri mu gipimo ihuzwa na antibody ya anti EV71 yanditseho fluorescence, maze igakora uruvange rw'ubudahangarwa bw'umubiri. Mu gihe cy'imikorere ya chromatography, urujya n'uruza rw'uruvange mu cyerekezo cy'impapuro zifata, iyo complex inyuze mu gace k'igerageza, ihujwe na antibody ya anti EV71, ikora complex nshya.

Iyo ari nta bimenyetso, icyitegererezo ntikiba kirimo antibody ya enterovirus 71 IgM, bityo ubudahangarwa bw'umubiri ntibushobora kubaho. Nta murongo utukura uzaba uri mu gace ko gupima (T). Uko antibody ya Enterovirus 71 IgM yaba iri muri specimen cyangwa idafite, antibody yasigaye ya IgM monoclonal ya mbeba ifite ikirango cya zahabu hamwe na antibody ya IgG ya mbeba ya antibody ya IgG itwikiriwe mu gace ko kugenzura ubuziranenge (C) birafatana. Hanyuma agglutinates zigira ibara mu gace ko kugenzura ubuziranenge, maze umurongo utukura ukagaragara muri (C). Umurongo utukura ni wo ugaragara mu gace ko kugenzura ubuziranenge (C) kugira ngo harebwe niba hari ingero zihagije kandi niba inzira ya chromatography ari isanzwe. Ikoreshwa kandi nk'igipimo cy'igenzura ry'imbere ku binyabutabire.

Uburyo bwo gupima:

1. Ingero zapimwe zishobora kuba amaraso yose, harimo amaraso yo mu mitsi cyangwa amaraso yo mu nkengero z'amaraso. Amaraso yose ntashobora kubikwa nyuma yo kuyafata. Nkwiye kuyakoresha vuba nyuma yo kuyafata.

2. Ingero za serum zikusanywa mu buryo busanzwe hakurikijwe uburyo busanzwe bwo gukoresha. Serum idakoresha ubushyuhe ntishobora gukoreshwa. Ntibyemewe gukoresha serum irimo ibinure, ifu cyangwa yanduye. Ifite uduce duto mu serum. Kandi imvura izagira ingaruka ku bisubizo by'ibizamini, ingero nk'izo zigomba kuyungururwa cyangwa kuyungururwa mbere yo gukoreshwa.

3. Ingero zapimwe zishobora kuba heparin, Sodium citrate cyangwa EDTA anticoagulant plasma.

4. Dukurikije uburyo busanzwe bwo gukusanya icyitegererezo. Icyitegererezo cya serum cyangwa plasma gishobora kubikwa muri firigo kuri 2-8°C mu gihe cy'iminsi 3 no kubikwa munsi ya -15°C mu gihe cy'amezi 3.

5. Ingero zose zirinda gukonjesha no gushonga.