UMGAQO

I-membrane yesixhobo sovavanyo igqunywe nge-anti-EV71 antibody kwindawo yovavanyo kunye ne-anti-rabbit IgG antibody yebhokhwe kwindawo yokulawula. Iileyibhile zigqunywe nge-anti-EV71 antibody ebhalwe nge-fluorescence kunye ne-IgG yomvundla kwangaphambili. Xa kuvavanywa isampuli entle, i-EV71 antigen kwisampuli idibana ne-anti-EV71 antibody ebhalwe nge-fluorescence, kwaye yenze umxube wokuzikhusela. Phantsi kwesenzo se-chromatography, ukuhamba kwe-complex kwicala lephepha elifunxayo, xa i-complex idlula kwindawo yovavanyo, idibene ne-anti-EV71 coating antibody, yenza i-complex entsha.

Ukuba ayibonakali, isampuli ayinayo i-antibody ye-enterovirus 71 IgM, ukuze i-immune complex ingakwazi ukwakheka. Akuyi kubakho mgca ubomvu kwindawo yokubona (T). Nokuba i-antibody ye-Enterovirus 71 IgM ikhona kwisampuli okanye ayikho, i-antibody ye-mouse anti-human IgM monoclonal eseleyo kunye ne-antibody ye-mouse anti-mouse IgG egqunywe kwindawo yokulawula umgangatho (C) ziyabopha. Emva koko ii-agglutinates zivelisa umbala kwindawo yokulawula umgangatho, kwaye umgca obomvu uya kuvela kwi-(C). Umgca obomvu ngumgangatho obonakala kwindawo yokulawula umgangatho (C) wokugweba ukuba kukho iisampulu ezaneleyo kunye nokuba inkqubo ye-chromatography iqhelekile na. Ikwasetyenziswa njengomgangatho wolawulo lwangaphakathi lwee-reagents.

Inkqubo yoVavanyo:

1. Iisampulu ezivavanyiweyo zingaba ligazi elipheleleyo, kuquka igazi eliphuma kwimithambo yegazi okanye igazi eliphuma kwimithambo yegazi. Igazi elipheleleyo alinakugcinwa emva kokuqokelelwa. Ndifanele ndilisebenzise ngokukhawuleza emva kokuqokelelwa.

2. Iisampuli ze-serum ziqokelelwa ngendlela engenatyhefu ngokweendlela eziqhelekileyo. I-serum engenabushushu ayinakusetyenziswa. Akukhuthazwa ukusebenzisa i-lipemic, turbid okanye i-serum engcolileyo. Izinto ezincinci kwi-serum. Kwaye ukuna kwemvula kuya kuchaphazela iziphumo zovavanyo, ezo sampuli kufuneka zifakwe kwi-centrifuge okanye zihluzwe ngaphambi kokusetyenziswa.

3.Iisampulu ezivavanyiweyo zinokuba yi-heparin, i-Sodium citrate okanye i-EDTA anticoagulant plasma.

4. Ngokweendlela eziqhelekileyo zokuqokelela isampuli. Isampuli ye-serum okanye ye-plasma ingagcinwa efrijini kwi-2-8℃ iintsuku ezi-3 kwaye igcinwe ngaphantsi kwe-15°C iinyanga ezi-3.

5. Zonke iisampuli ziphephe imijikelo yokuqandisa nokunyibilikisa.