Ikhithi Yokuxilonga yeI-Luteinizing Hormoneukuhlolwa kwe-fluorescence immunochromatographic)
Ukusetshenziswa kokuxilonga ngaphakathi kwesibeletho kuphela
Sicela ufunde lesi sithasiselo sephakheji ngokucophelela ngaphambi kokusebenzisa futhi ulandele imiyalelo ngokucophelela. Ukuthembeka kwemiphumela yokuhlola akunakuqinisekiswa uma kukhona ukuphambuka emiyalweni ekulesi sithasiselo sephakheji.

UKUSETSHENZISWA OKUHLOSIWE
Ikhithi Yokuxilonga ye-Luteinizing Hormone (i-fluorescence immunochromatographic assay) iyi-fluorescence immunochromatographic assay yokuthola inani le-Luteinizing Hormone (LH) ku-serum noma ku-plasma yomuntu, esetshenziswa kakhulu ekuhlolweni komsebenzi we-endocrine ye-pituitary. Lonke isampula elihle kumele liqinisekiswe ngezinye izindlela. Lokhu kuhlolwa kuhloselwe ukusetshenziswa kochwepheshe bezempilo kuphela.

ISIFINYEZO

I-Luteinizing hormone (LH) iyi-glycoprotein enesisindo sama-molecule esingama-Dalton angu-30,000, ekhiqizwa yi-anterior pituitary. Ukuhlushwa kwe-LH kuhlobene kakhulu nokukhishwa kwamaqanda, futhi inani eliphakeme le-LH libikezelwa ukuthi lizoba amahora angama-24 kuya kwangu-36 okukhishwa kwamaqanda. Ngakho-ke, inani eliphakeme le-LH lingaqashwa ngesikhathi somjikelezo wokuya esikhathini ukuze kunqunywe isikhathi esifanele sokukhulelwa. Umsebenzi ongavamile we-endocrine ku-pituitary gland ungabangela ukungalingani kokukhishwa kwe-LH. Ukuhlushwa kwe-LH kungasetshenziswa ukuhlola umsebenzi we-endocrine we-pituitary. I-Diagnostic Kit isekelwe ku-immunochromatography futhi inganikeza umphumela zingakapheli imizuzu eyi-15.

ISIMISO SENQUBO

I-membrane yedivayisi yokuhlola imbozwe nge-anti-LH antibody endaweni yokuhlola kanye ne-anti-IgG antibody yembuzi endaweni yokulawula. I-lable pad imbozwe nge-anti-LH antibody ebhalwe nge-fluorescence kanye ne-IgG yenogwaja kusengaphambili. Lapho kuhlolwa isampula enhle, i-antigen ye-LH kusampula ihlangana ne-anti-LH antibody ebhalwe nge-fluorescence, bese yakha ingxube ye-immune. Ngaphansi kwesenzo se-immunochromatography, ukugeleza okuyinkimbinkimbi kuya ohlangothini lwephepha elimunca, lapho i-complex idlula endaweni yokuhlola, ihlanganiswe ne-anti-LH coating antibody, yakha i-complex entsha. Izinga le-LH lihlotshaniswa kahle nesignali ye-fluorescence, futhi ukuhlushwa kwe-LH kusampula kungatholakala nge-fluorescence immunoassay assay.

IZINTO EZINGENAYO NEZINTO EZINIKEZWE

Izingxenye zephakheji ezingama-25T：

Ikhadi lokuhlola ngalinye lifakwe ifoyili ngesikhwama se-desiccant esingu-25T
.Izibonelo zokunciphisa
.Iphakheji yokufaka

KUDINGEKA IZINTO EZISETSHENZISWAYO KODWA AZINIKEZWA
Isitsha sokuqoqa isampula, isibali sikhathi

IQOQO LEZIBONELO NOKUGCINA
1. Amasampula ahlolwe angaba yi-serum, i-heparin anticoagulant plasma noma i-EDTA anticoagulant plasma.
2. Ngokwezindlela ezijwayelekile zokuqoqa isampula. Isampula ye-serum noma ye-plasma ingagcinwa efrijini ku-2-8℃ izinsuku eziyi-7 kanye nokugcinwa ngaphansi kuka-15°C izinyanga eziyi-6.
3. Zonke izibonelo zigwema imijikelezo yokuqandisa nokuncibilikisa.

INQUBO YOKUHLOLA

Inqubo yokuhlola yale thuluzi bheka incwadi ye-immunoanalyzer. Inqubo yokuhlola i-reagent imi kanje
1. Beka eceleni wonke ama-reagent namasampula ukuze izinga lokushisa legumbi lifinyelele ezingeni lokushisa legumbi.
2.Vula i-Portable Immune Analyzer (WIZ-A101), faka ukungena ngemvume kwephasiwedi ye-akhawunti ngokwendlela yokusebenza kwethuluzi, bese ufaka isikhombikubona sokuthola.
3.Skena ikhodi yokuqinisekisa ukuze uqinisekise into yokuhlola.
4. Khipha ikhadi lokuhlola esikhwameni se-foil.
5.Faka ikhadi lokuhlola embobeni yekhadi, skena ikhodi ye-QR, bese unquma into yokuhlolwa.
6. Faka isampula ye-serum noma ye-plasma engu-20μL kusampula yokuxuba, bese uxuba kahle.
7. Engeza isisombululo sesampula esingu-80μL emthonjeni wesampula wekhadi.
8.Chofoza inkinobho ethi “ukuhlolwa okujwayelekile”, ngemva kwemizuzu eyi-15, ithuluzi lizobona ngokuzenzakalelayo ikhadi lokuhlola, lingafunda imiphumela esikrinini sokubonisa sethuluzi, bese liqopha/liphrinta imiphumela yokuhlolwa.
9.Bheka imiyalelo ye-Portable Immune Analyzer (WIZ-A101).

IMIPHUMELA YOKUHLOLA NOKUHLUKANISWA

.Idatha engenhla iyisikhathi sokubhekisela esimiselwe idatha yokuthola yale khithi, futhi kuphakanyiswa ukuthi ilabhorethri ngayinye kufanele isungule isikhathi sokubhekisela sokubaluleka komtholampilo okufanelekile kwabantu kulesi sifunda.
.Ukuhlushwa kwe-LH kuphakeme kunobubanzi bokubhekisela, futhi izinguquko zomzimba noma impendulo yokucindezeleka kufanele ikhishwe. Ngempela okungavamile, kufanele kuhlanganiswe ukuxilongwa kwezimpawu zomtholampilo.
.Imiphumela yale ndlela isebenza kuphela kububanzi bokubhekisela obusungulwe yile ndlela, futhi imiphumela ayifani ngqo nezinye izindlela.
Ezinye izici zingadala amaphutha emiphumeleni yokuthola, okuhlanganisa izizathu zobuchwepheshe, amaphutha okusebenza kanye nezinye izici zesampula.

ISITOKELO NOKUZINZIMA
1. Ikhithi ihlala izinyanga ezingu-18 kusukela ngosuku lokwenziwa kwayo. Gcina amakhithi angasetshenziswanga ku-2-30°C. UNGAWAQANDISI. Ungayisebenzisi ngemva kosuku lokuphelelwa yisikhathi.
2. Ungavuli isikhwama esivaliwe uze ulungele ukwenza isivivinyo, futhi isivivinyo sokusetshenziswa kanye sinconywa ukuthi sisetshenziswe ngaphansi kwendawo edingekayo (izinga lokushisa 2-35℃, umswakama 40-90%) zingakapheli imizuzu engama-60 ngokushesha okukhulu.
3. Isampula yokuxuba isetshenziswa ngokushesha ngemva kokuvulwa.

IZIXWAYISO NEZIQAPHELO ZOKWENZA
.Ikhithi kufanele ivalwe futhi ivikelwe emswakama.
Zonke izibonelo ezinhle kufanele ziqinisekiswe ngezinye izindlela.
Zonke izibonelo kufanele ziphathwe njengezingcolisi ezingaba khona.
UNGASEBENZISI i-reagent ephelelwe yisikhathi.
AKUFANELE ukushintshaniswe ama-reagent phakathi kwamakhithi anenombolo ehlukile.
UNGASEBENZISI kabusha amakhadi okuhlola nanoma yiziphi izesekeli ezilahlwayo.
.Ukusebenza kabi, isampula eningi kakhulu noma encane kungaholela ekuphambukeni kwemiphumela.

LUKULINGISA
.Njenganoma yikuphi ukuhlolwa okusebenzisa ama-antibodies egundane, kungenzeka ukuthi kukhona ukuphazamiseka kwama-antibodies e-anti-mouse (HAMA) esampula. Amasampula avela ezigulini ezithole amalungiselelo ama-antibodies e-monoclonal ukuze kutholakale isifo noma ukwelashwa angase abe ne-HAMA. Amasampula anjalo angabangela imiphumela emibi noma emibi engamanga.
.Lo mphumela wokuhlolwa ungowokubhekisela emtholampilo kuphela, akufanele usebenze njengesisekelo sokuxilongwa nokwelashwa kwezokwelapha kuphela, ukuphathwa kwezokwelapha kweziguli kufanele kucatshangelwe ngokuphelele kuhlanganiswe nezimpawu zaso, umlando wezokwelapha, okunye ukuhlolwa kwelabhorethri, impendulo yokwelashwa, i-epidemiology kanye nolunye ulwazi.
.Le reagent isetshenziswa kuphela ekuhlolweni kwe-serum kanye ne-plasma. Ingase ingatholi umphumela onembile uma isetshenziswa kwamanye amasampula afana namathe nomchamo njll.

IZIMALI ZOKUSEBENZA

IZINKOMBA
1.Hansen JH, nabanye.Ukuphazamiseka kwe-HAMA nge-Murine Monoclonal Antibody-Based Immunoassays[J].J ye-Clin Immunoassay,1993,16:294-299.
2.Levinson SS. Uhlobo Lwama-Antibodies Angama-Heterophilic kanye Nendima Ekuphazamisekeni Kwe-Immunoassay[J].J we-Clin Immunoassay,1992,15:108-114.

Isihluthulelo sezimpawu ezisetshenzisiwe:

	Idivayisi Yezokwelapha Yokuxilonga E-In Vitro
	Umkhiqizi
	Gcina ku-2-30℃
	Usuku lokuphelelwa isikhathi
	Ungasebenzisi Kabusha
	ISEXWAYISO
	Bheka Imiyalelo Yokusebenzisa

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